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欧美山杨杂种插穗不定根2个发育时期基因表达谱分析

闫绍鹏 尚艳茹 冷淑娇 杨瑞华 王秋玉

闫绍鹏, 尚艳茹, 冷淑娇, 杨瑞华, 王秋玉. 欧美山杨杂种插穗不定根2个发育时期基因表达谱分析[J]. 北京林业大学学报, 2015, 37(10): 9-13. doi: 10.13332/j.1000-1522.20130230
引用本文: 闫绍鹏, 尚艳茹, 冷淑娇, 杨瑞华, 王秋玉. 欧美山杨杂种插穗不定根2个发育时期基因表达谱分析[J]. 北京林业大学学报, 2015, 37(10): 9-13. doi: 10.13332/j.1000-1522.20130230
YAN Shao-peng, SHANG Yan-ru, LENG Shu-jiao, YANG Rui-hua, WANG Qiu-yu. Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.[J]. Journal of Beijing Forestry University, 2015, 37(10): 9-13. doi: 10.13332/j.1000-1522.20130230
Citation: YAN Shao-peng, SHANG Yan-ru, LENG Shu-jiao, YANG Rui-hua, WANG Qiu-yu. Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.[J]. Journal of Beijing Forestry University, 2015, 37(10): 9-13. doi: 10.13332/j.1000-1522.20130230

欧美山杨杂种插穗不定根2个发育时期基因表达谱分析

doi: 10.13332/j.1000-1522.20130230
基金项目: 

国家自然科学基金项目(J1210053)、中央高校基本科研业务费专项(2572014CA22)。

详细信息
    作者简介:

    闫绍鹏,博士,高级工程师。主要研究方向:林木遗传学。Email:ysp_4@126.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学生命科学学院。 责任作者: 王秋玉,博士,教授。主要研究方向:林木遗传学。Email:wqyll@sina.com.cn 地址:同上。

    闫绍鹏,博士,高级工程师。主要研究方向:林木遗传学。Email:ysp_4@126.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学生命科学学院。 责任作者: 王秋玉,博士,教授。主要研究方向:林木遗传学。Email:wqyll@sina.com.cn 地址:同上。

Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.

  • 摘要: 以欧美杂种山杨嫩枝插穗为实验材料,进行扦插不定根生成过程中插穗形态和解剖学观察,通过Solexa高通量测序对扦插后6和12 d插穗不定根生成部位进行转录水平上的检测分析。插穗解剖观察发现扦插后6 d为根原基的诱导期,12 d为不定根的伸长生长期,对插穗不定根2个时期高通量测序获得差异表达基因2 328个。对筛选出的显著差异表达基因进行功能注释、分类和KEGG及pathway分析,确定2个山杨样本中差异表达基因参与的生化代谢和信号转导途径。

     

  • [1] 闫绍鹏.欧美山杨杂种无性系低温胁迫下几种生理指标的遗传变异[D].哈尔滨:东北林业大学,2004.
    [1] YAN S P. Genetic variation among the hybrid clones of Populus tremula × P. tremloides in several physiological characteristics under low temperature stress[D].Harbin: Northeast Forestry University,2004.
    [2] ROSIER C L,FRAMPTON J, GOLDFARB B,et al. Growth stage,auxin type,and concentration,influence rooting of Virginia pine stem cuttings[J]. Hortscience,2004,39(6):1397-1402.
    [2] LENG S J. Analysis of the digital gene expression profiling of Populus tremula × P. tremloides cuttings development during adventitious roots induction and adventitious roots elongation[D]. Harbin: Northeast Forestry University,2012.
    [3] YAN S P, YANG R H, LENG S J, et al. High-fluxed DNA sequencing technology and its application in agricultural science research[J]. Chinese Agricultural Science Bulletin, 2012,28(30): 171-176.
    [3] SYROS T,YUPSANIS T,ZAFIRIADIS H,et al. Activity and isoforms of peroxidases,lignin and anatomy,during adventitious rooting in cuttings of Ebenus cretiea L[J]. Journal of Plant Physiology,2004,16(1):69-77.
    [4] 冷淑娇.欧美山杨插穗不定根发育的诱导期和伸长期数字表达谱分析[D].哈尔滨:东北林业大学,2012.
    [5] BRINKER M, ZYL L, LIU W B, et al. Microarray analyses of gene expression during adventitious root development in Pinus contorta[J]. Plant Physiology, 2004, 135:1526-1539.
    [6] RIGAL A, YORDANOV Y S, PERRONE I, et al. The AINTEGUMENTA LIKE1 homeotic transcription factor PtAIL1 controls the formation of adventitious root primordia in poplar[J]. Plant Physiol, 2012, 160:1996-2006.
    [7] LI R Q,YU C,LI Y R,et al. SOAP2: an improved ultrafast tool for short read alignment[J]. Bioinformatics,2009, 25(15): 1966-1967.
    [8] MORTAZAVI A, WILLIAMS B A, MCCUE K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature Methods,2008,5(7):621-628.
    [9] THOMAS P, SCHIEFELBEIN J. Cloning and characterization of an actin depolymerizing factor gene from grape (Vitis inifera L.) expressed during rooting in stem cuttings[J]. Plant Science, 2002, 162:283-288.
    [10] SWARUP R, FRIML J, MARCHANT A, et al. Localization of the auxin permease AUX1 suggests two functionally distinct hormone transport pathways operate in the Arabidopsis root apex[J]. Genes Dev, 2001,15: 2648-2653.
    [11] WU G S, DANIEL R L, EDGAR P. Spalding, mutations in Arabidopsis multidrug resistance-like ABC transporters separate the roles of acropetal and basipetal auxin transport in lateral root development[J]. The Plant Cell, 2007,19(6): 1826-1837.
    [12] DING X H, CAO Y L, HUANG L L, et al. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate-and jasmonate-independent basal immunity in rice[J]. The Plant Cell January,2008,20(1):228-240.
    [13] 闫绍鹏,杨瑞华,冷淑娇,等. 高通量测序技术及其在农业科学研究中的应用[J].中国农学通报,2012,28(30):171-176.
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出版历程
  • 收稿日期:  2013-12-25
  • 刊出日期:  2015-10-31

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