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四倍体桦树树皮中三萜化合物的测定与评价

王遂 赵慧 杨传平 姜静

王遂, 赵慧, 杨传平, 姜静. 四倍体桦树树皮中三萜化合物的测定与评价[J]. 北京林业大学学报, 2015, 37(9): 53-61. doi: 10.13332/j.1000-1522.20140150
引用本文: 王遂, 赵慧, 杨传平, 姜静. 四倍体桦树树皮中三萜化合物的测定与评价[J]. 北京林业大学学报, 2015, 37(9): 53-61. doi: 10.13332/j.1000-1522.20140150
WANG Sui, ZHAO Hui, YANG Chuan-ping, JIANG Jing.. Analysis and evaluation of triterpenoids in tetraploid birch (Betula).[J]. Journal of Beijing Forestry University, 2015, 37(9): 53-61. doi: 10.13332/j.1000-1522.20140150
Citation: WANG Sui, ZHAO Hui, YANG Chuan-ping, JIANG Jing.. Analysis and evaluation of triterpenoids in tetraploid birch (Betula).[J]. Journal of Beijing Forestry University, 2015, 37(9): 53-61. doi: 10.13332/j.1000-1522.20140150

四倍体桦树树皮中三萜化合物的测定与评价

doi: 10.13332/j.1000-1522.20140150
基金项目: 

十二五国家科技支撑计划课题(2012BAD21B02)。

详细信息
    作者简介:

    王遂。主要研究方向:林木遗传育种。Email: wangsui.ws@163.com 地址:150040黑龙江省哈尔滨市和兴路51号东北林业大学林木遗传育种国家重点实验室。

    责任作者:

    姜静,教授,博士生导师。主要研究方向:林木遗传育种。Email: jiangjing1960@126.com 地址:同上。

Analysis and evaluation of triterpenoids in tetraploid birch (Betula).

  • 摘要: 白桦脂酸、齐墩果酸和白桦脂醇是桦树树皮中含量较高的3种三萜类化合物,具有重要的药用价值。本实验以超声波辅助提取法提取桦树树皮中的三萜化合物,并利用高效液相色谱法分离检测3种三萜化合物,进而构建小提琴图和星相图等研究2种四倍体桦树树皮中3种三萜化合物的含量差异,并通过因子分析法,筛选出综合性状优良的四倍体桦树单株。结果表明:三萜化合物分离、检测的色谱条件为以十八烷基硅烷键合硅胶为填充剂的C18色谱柱(4.6 mm250 mm5 m),以乙腈-水(含0.1%磷酸)为流动相,梯度洗脱,柱温30 ℃,流速0.5 mL/min,检测波长为195 nm;在0.05的显著性水平下,2种四倍体桦树树皮中齐墩果酸的含量存在显著差异,在0.10的显著性水平下,2种四倍体桦树树皮中白桦脂酸和白桦脂醇的含量存在显著差异,其中杂种白桦中3种三萜化合物含量普遍高于白桦;3种三萜化合物间存在极显著的正相关关系,其中白桦脂酸和白桦脂醇间的相关系数最高,为0.911,而白桦脂醇和齐墩果酸间的相关系数最低,为0.631;利用因子分析法构建了F=48.620%F1+21.080%F2+21.075%F3桦树三萜综合评价公式,并筛选出了综合性状相对优良的桦树单株,其中白桦中综合得分最高的前3个单株依次为4126、4123、4125,杂种白桦中得分最高的前3个单株依次为4202、4204、4208。

     

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出版历程
  • 收稿日期:  2014-04-27
  • 刊出日期:  2015-09-30

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