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小叶黄杨冬季叶片呈色与其类胡萝卜素及活性氧关系研究

姜雪茹 彭金根 郭翎 高荣孚 刘燕

姜雪茹, 彭金根, 郭翎, 高荣孚, 刘燕. 小叶黄杨冬季叶片呈色与其类胡萝卜素及活性氧关系研究[J]. 北京林业大学学报, 2015, 37(6): 93-99. doi: 10.13332/j.1000-1522.20140258
引用本文: 姜雪茹, 彭金根, 郭翎, 高荣孚, 刘燕. 小叶黄杨冬季叶片呈色与其类胡萝卜素及活性氧关系研究[J]. 北京林业大学学报, 2015, 37(6): 93-99. doi: 10.13332/j.1000-1522.20140258
JIANG Xue-ru, PENG Jin-gen, GUO Ling, GAO Rong-fu, LIU Yan. Relationship between winter leaf reddening and carotenoids, reactive oxygen species in Buxus microphylla L[J]. Journal of Beijing Forestry University, 2015, 37(6): 93-99. doi: 10.13332/j.1000-1522.20140258
Citation: JIANG Xue-ru, PENG Jin-gen, GUO Ling, GAO Rong-fu, LIU Yan. Relationship between winter leaf reddening and carotenoids, reactive oxygen species in Buxus microphylla L[J]. Journal of Beijing Forestry University, 2015, 37(6): 93-99. doi: 10.13332/j.1000-1522.20140258

小叶黄杨冬季叶片呈色与其类胡萝卜素及活性氧关系研究

doi: 10.13332/j.1000-1522.20140258
基金项目: 

北京市科委项目(Z08050602970801)。

详细信息
    作者简介:

    姜雪茹。主要研究方向:园林植物生理生态。 Email: hnfgjxr@163.com 地址:100083北京市清华东路35号北京林业大学园林学院。责任作者: 刘燕,教授,博士生导师。主要研究方向:园林花卉栽培与应用。 Email: chbly@163.com 地址:同上。

    姜雪茹。主要研究方向:园林植物生理生态。 Email: hnfgjxr@163.com 地址:100083北京市清华东路35号北京林业大学园林学院。责任作者: 刘燕,教授,博士生导师。主要研究方向:园林花卉栽培与应用。 Email: chbly@163.com 地址:同上。

Relationship between winter leaf reddening and carotenoids, reactive oxygen species in Buxus microphylla L

  • 摘要: 研究常绿阔叶植物冬季变色的生理生化响应对揭示其冬季变色机制具有理论意义,也可为北方冬季绿色景观维护起实际指导作用。本文以‘丽城’小叶黄杨植株冬季不同光照下呈现绿色(GG)、绿棕色(GB)、棕色(BB)、红棕色(RB)和亮红色(RR)5种颜色叶片为对象,研究了不同呈色叶片的色素含量、分布与其活性氧指标(活性氧含量、抗氧化酶活性及氧化损伤程度)的关系。结果表明:绿色(GG)叶片叶肉组织全部呈现绿色,红色叶片在栅栏组织外部细胞层及海绵组织下部细胞层均有不同范围的红色区域出现,且红色深度和范围与叶片表型颜色相一致;与绿色叶片GG相比,冬季变色叶片(GB、BB、RB、RR)类胡萝卜素(Car)含量显著增加,光抑制程度明显加重,活性氧相关指标如过氧化氢(H2O2)含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著升高,且叶片越红,PSⅡ光化学效率越小,Car和H2O2含量越高,两者呈显著正相关;总叶绿素[Chl(a+b)]含量RR最低,RB最高;不同颜色叶片间过氧化物酶(POD)活性变化无一定规律。此外,红色程度最深的RB、RR两种叶片具有最低的超氧自由基(O-2)及丙二醛(MDA)含量,且O-2与Car呈显著负相关。综上所述,不同呈色叶片Car的积累量不同,并且与H2O2水平密切相关,H2O2的增加可能促进了抗氧化酶SOD、CAT活性及Car含量的上调,使红色叶片免受氧化伤害。‘丽城’小叶黄杨冬季叶片呈色与活性氧密切相关,但是不同活性氧种类作用不同,H2O2在其中可能起着积极作用,值得进一步研究。

     

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  • 收稿日期:  2014-07-23

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