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棘孢木霉可湿性粉剂研制及杀菌活性测定

孙丽丽 曹传旺 薛绪亭 王志英 杜春艳

孙丽丽, 曹传旺, 薛绪亭, 王志英, 杜春艳. 棘孢木霉可湿性粉剂研制及杀菌活性测定[J]. 北京林业大学学报, 2015, 37(6): 45-52. doi: 10.13332/j.1000-1522.20140438
引用本文: 孙丽丽, 曹传旺, 薛绪亭, 王志英, 杜春艳. 棘孢木霉可湿性粉剂研制及杀菌活性测定[J]. 北京林业大学学报, 2015, 37(6): 45-52. doi: 10.13332/j.1000-1522.20140438
SUN Li-li, CAO Chuan-wang, XUE Xu-ting, WANG Zhi-ying, DU Chun-yan. Preparation and fungicidal bioactivity of wettable powder formulations of Trichoderma asperellum[J]. Journal of Beijing Forestry University, 2015, 37(6): 45-52. doi: 10.13332/j.1000-1522.20140438
Citation: SUN Li-li, CAO Chuan-wang, XUE Xu-ting, WANG Zhi-ying, DU Chun-yan. Preparation and fungicidal bioactivity of wettable powder formulations of Trichoderma asperellum[J]. Journal of Beijing Forestry University, 2015, 37(6): 45-52. doi: 10.13332/j.1000-1522.20140438

棘孢木霉可湿性粉剂研制及杀菌活性测定

doi: 10.13332/j.1000-1522.20140438
基金项目: 

“十二五”国家科技支撑计划项目(2011BAD37B01-4)、黑龙江省自然科学基金项目(C201409)、吉林省留学人员科技创新创业项目(2013-36)、中央高校基本科研业务费专项基金项目(2572014AA31)。

详细信息
    作者简介:

    孙丽丽,博士生。主要研究方向:森林病虫害生物防治。Email:253035020@qq.com 地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学林学院。责任作者: 王志英,教授,博士生导师。主要研究方向:森林病虫害生物防治。Email:zyw0451@sohu.com 地址:同上。曹传旺,博士,副教授。主要研究方向:昆虫毒理学。Email:chuanwangcao@126.com 地址:同上。

    孙丽丽,博士生。主要研究方向:森林病虫害生物防治。Email:253035020@qq.com 地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学林学院。责任作者: 王志英,教授,博士生导师。主要研究方向:森林病虫害生物防治。Email:zyw0451@sohu.com 地址:同上。曹传旺,博士,副教授。主要研究方向:昆虫毒理学。Email:chuanwangcao@126.com 地址:同上。

    孙丽丽,博士生。主要研究方向:森林病虫害生物防治。Email:253035020@qq.com 地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学林学院。责任作者: 王志英,教授,博士生导师。主要研究方向:森林病虫害生物防治。Email:zyw0451@sohu.com 地址:同上。曹传旺,博士,副教授。主要研究方向:昆虫毒理学。Email:chuanwangcao@126.com 地址:同上。

Preparation and fungicidal bioactivity of wettable powder formulations of Trichoderma asperellum

  • 摘要: 以棘孢木霉分生孢子粉、发酵液冻干粉为有效成分制备混配杀菌剂。在测定助剂对棘孢木霉生物活性的基础上,利用正交试验设计法测定助剂对制剂性能的影响,进一步确定助剂种类和含量;通过机械粉碎法将棘孢木霉分生孢子粉、发酵液冻干粉与助剂混合配比加工成可湿性粉剂。室内离体试验法测定棘孢木霉分生孢子粉和发酵液冻干粉不同配比的制剂对油菜菌核病菌抑菌活性,获得最佳比例,并将其加工成可湿性粉剂。结果表明:棘孢木霉孢子粉和发酵液冻干粉可湿性粉剂最佳配比(质量比)为1∶1,润湿分散剂为1% Morwet EFW、5% TERWET 1010、5% Morwet D425、7%木质素磺酸钙;紫外保护剂为0.3%纳米氧化锌,以硅藻土为载体补足100%。该配方可湿性粉剂的孢子萌发率为88.57%,质量悬浮率达81.79%,孢子悬浮率80.12%,润湿时间10 s,平均粒径27 μm,符合商品制剂的标准。室内生物测定结果表明,不同混配比例制剂均有一定的增效作用,但该配比可湿性粉剂增效作用最为显著,其中1 600倍液对油菜菌核病菌防治效果比孢子粉、发酵液冻干粉单剂分别高21.67%和12.09%,抑制率范围为64.17%~88.67%。该制剂属于环境友好型绿色农药,为农林植物病害的生物防治提供了一种新生防材料。

     

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