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白桦BpHO基因非生物胁迫及信号诱导的表达模式分析

周姗 李思达 董恒 詹亚光 曾凡锁

周姗, 李思达, 董恒, 詹亚光, 曾凡锁. 白桦BpHO基因非生物胁迫及信号诱导的表达模式分析[J]. 北京林业大学学报, 2015, 37(11): 69-75. doi: 10.13332/j.1000-1522.20140485
引用本文: 周姗, 李思达, 董恒, 詹亚光, 曾凡锁. 白桦BpHO基因非生物胁迫及信号诱导的表达模式分析[J]. 北京林业大学学报, 2015, 37(11): 69-75. doi: 10.13332/j.1000-1522.20140485
ZHOU Shan, LI Si-da, DONG Heng, ZHAN Ya-guang, ZENG Fan-suo.. Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.[J]. Journal of Beijing Forestry University, 2015, 37(11): 69-75. doi: 10.13332/j.1000-1522.20140485
Citation: ZHOU Shan, LI Si-da, DONG Heng, ZHAN Ya-guang, ZENG Fan-suo.. Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.[J]. Journal of Beijing Forestry University, 2015, 37(11): 69-75. doi: 10.13332/j.1000-1522.20140485

白桦BpHO基因非生物胁迫及信号诱导的表达模式分析

doi: 10.13332/j.1000-1522.20140485
基金项目: 

中央高校基本科研业务费专项资金项目(2572014DA04)、国家自然科学基金项目(J1210053、31200463)、黑龙江省博士后启动基金项目(LBH-Q12166)。

详细信息
    作者简介:

    周姗。主要研究方向:植物基因工程。Email:zhoushanleo@163.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学生命科学学院。
    责任作者:曾凡锁,副教授。主要研究方向:林木遗传育种和生物技术。Email:zengfansuo@126.com 地址:同上。

    周姗。主要研究方向:植物基因工程。Email:zhoushanleo@163.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学生命科学学院。
    责任作者:曾凡锁,副教授。主要研究方向:林木遗传育种和生物技术。Email:zengfansuo@126.com 地址:同上。

Analysis of the expression patterns of gene induced by abiotic stress and signal in birch.

  • 摘要: 血红素加氧酶(heme oxygenase, HO)是一种普遍存在的敏感的和高活性的血红素分解代谢过程中的限速酶。本文揭示了白桦BpHO在非生物胁迫及信号诱导调控的表达特征,为该基因在白桦中代谢调控功能的研究奠定基础。通过前期研究获得了白桦HO基因,命名为BpHO,应用生物信息学软件分析了白桦BpHO基因的分子结构特征,利用重金属镉(Cd)、盐(NaCl)以及4 ℃低温进行非生物胁迫处理,利用硝普钠(SNP)和水杨酸(SA)进行信号诱导,分析BpHO的表达特征。生物信息学分析表明该基因全长855 bp,含有完整的开放读码框,编码284个氨基酸(Genebank登录号:KJ197335)。BpHO为不稳定类疏水性蛋白,不存在信号肽,也不具有跨膜能力,α-螺旋、延伸链、无规则卷曲分布于整个蛋白。分子进化分析结果表明,白桦BpHO基因与葡萄的遗传距离较近,说明二者亲缘关系较近;与大豆、菜豆、豌豆的遗传距离较远,说明其亲缘关系较远。非生物胁迫结果表明,BpHO基因的表达水平随非生物胁迫处理时间的不同而上下波动,但在处理6 h后BpHO基因对4种非生物胁迫处理都上调表达,说明BpHO基因对非生物胁迫具有响应。信号诱导结果表明,外源NO调节了BpHO基因的转录表达,调控血红素加氧酶的合成。SA也参与了BpHO基因的信号调控。盐、低温、重金属镉胁迫均能促进BpHO 的表达,但响应模式不同。

     

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出版历程
  • 收稿日期:  2014-12-05
  • 刊出日期:  2015-11-30

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