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转基因白桦杂种T1 代的生长发育及AP1 基因的遗传分析

王朔 黄海娇 杨光 姜静 刘桂丰

王朔, 黄海娇, 杨光, 姜静, 刘桂丰. 转基因白桦杂种T1 代的生长发育及AP1 基因的遗传分析[J]. 北京林业大学学报, 2016, 38(9): 1-7. doi: 10.13332/j.1000-1522.20150029
引用本文: 王朔, 黄海娇, 杨光, 姜静, 刘桂丰. 转基因白桦杂种T1 代的生长发育及AP1 基因的遗传分析[J]. 北京林业大学学报, 2016, 38(9): 1-7. doi: 10.13332/j.1000-1522.20150029
WANG Shuo, HUANG Hai-jiao, YANG Guang, JIANG Jing, LIU Gui-feng.. Growth and developmental analysis of T1 generation from BpAP1 transgenic birch.[J]. Journal of Beijing Forestry University, 2016, 38(9): 1-7. doi: 10.13332/j.1000-1522.20150029
Citation: WANG Shuo, HUANG Hai-jiao, YANG Guang, JIANG Jing, LIU Gui-feng.. Growth and developmental analysis of T1 generation from BpAP1 transgenic birch.[J]. Journal of Beijing Forestry University, 2016, 38(9): 1-7. doi: 10.13332/j.1000-1522.20150029

转基因白桦杂种T1 代的生长发育及AP1 基因的遗传分析

doi: 10.13332/j.1000-1522.20150029
基金项目: 

“863”国家高技术研究发展计划项目(2013AA102704)。

详细信息
    作者简介:

    王朔,博士生。主要研究方向:林木遗传育种。Email:wangshuo19910909@163.com地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者:刘桂丰,教授,博士生导师。主要研究方向:林木遗传育种。Email:liuguifeng@126.com地址:同上。

    王朔,博士生。主要研究方向:林木遗传育种。Email:wangshuo19910909@163.com地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者:刘桂丰,教授,博士生导师。主要研究方向:林木遗传育种。Email:liuguifeng@126.com地址:同上。

Growth and developmental analysis of T1 generation from BpAP1 transgenic birch.

  • 摘要: 本文以3株野生型白桦为母本、3株35S∷BpAP1转基因白桦及1株野生型白桦为父本进行杂交,调查杂种子代的生长发育、开花结实特性,分析外源AP1基因在T1代白桦中的遗传稳定性及分离规律。结果表明:BpAP1基因的插入对转基因植株的花粉活力略有影响,BpAP1基因可遗传给T1代的部分个体,遗传比例在36%~58%之间,经χ2检验,BpAP1基因的分离比符合孟德尔分离定律;遗传了BpAP1基因的白桦子代与父本一样仍然呈现出提前开花结实、明显矮化的特点,其1年生和2年生的高生长仅为野生型白桦杂种子代的44.19%和18.92%;T1代白桦存在2种完全不同的表型,据此能够简便快捷地判断出杂种子代是否携带有BpAP1基因。该研究证明了外源AP1基因可以通过有性生殖的方式稳定地遗传给子代,获得的转基因白桦杂种子代能够提早开花结实,因此35S∷BpAP1转基因白桦可作为研究白桦性状遗传规律的亲本材料。

     

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出版历程
  • 收稿日期:  2015-01-27
  • 刊出日期:  2016-09-30

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