Somatic embryogenesis of Liquidambar styraciflua induced from staminate flower and inflorescence axis
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摘要: 北美枫香是适应性强、生长速度快、秋叶红艳美丽的园林绿化树种。为了提高其繁殖效率,以北美枫香雄花和花序轴为外植体,采用完全随机区组设计,研究外植体类型,基因型,TDZ浓度对北美枫香愈伤组织的诱导、体胚分化和萌发的影响。结果表明:雄花作为外植体,合适的诱导培养基为改良Blaydes培养基+TDZ 0.15 mg/L+水解酪蛋白1 g/L+蔗糖40 g/L+3% 植物凝胶;花序轴作为外植体,合适的诱导培养基为改良Blaydes培养基+TDZ 0.01 mg/L+水解酪蛋白1 g/L+蔗糖40 g/L+3% 植物凝胶。合适的萌发培养基为不含水解酪蛋白的基本培养基。如果出现球形胚,将培养物转接到不含任何生长调节剂的改良Blaydes培养基上,体胚经成熟可进一步形成完整小植株。该体胚体系的建成将显著提高北美枫香的繁殖效率。Abstract: Sweetgum (Liquidambar styraciflua) is a garden landscaping tree with strong adaptability, fast growth, and red color of autumn leaves. In order to improve efficiency of its propagation, we used staminate flower and the inflorescence axis as explants to investigate effects of explant types, genotypes and TDZ concentration in culture media on callus and somatic embryo induction with random complete block design. The results showed that the optimal medium for induction of embryogenic callus was modified Blaydes medium+TDZ 0.15 mg/L+casein hydrolysate 1 g/L+surcrose 40 g/L+3% Phytagar(sigma) with staminate flower as explant. The optimal medium for induction of embryogenic callus was modified Blaydes medium+TDZ 0.01 mg/L+casein hydrolysate 1 g/L+surcrose 40 g/L+3% Phytagar(sigma) with inflorescence axis as explant. The suitable medium of germination was modified Blaydes medium without casein hydrolysate. Once globular-stage embryo formed, we transferred it to the same medium without plant growth regulators, and somatic embryos would mature and germinate to developed plantlets. The establishment of this somatic embryogenesis system will significantly improve propagation efficiency of Liquidambar styraciflua.
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Keywords:
- Liquidambar styraciflua /
- somatic embryogenesis /
- somatic embryo
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