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应用流式细胞术测定18个中国古老月季基因组大小

武荣花 葛蓓蓓 王茂良 周燕 冯慧

武荣花, 葛蓓蓓, 王茂良, 周燕, 冯慧. 应用流式细胞术测定18个中国古老月季基因组大小[J]. 北京林业大学学报, 2016, 38(6): 94-100. doi: 10.13332/j.1000-1522.20150364
引用本文: 武荣花, 葛蓓蓓, 王茂良, 周燕, 冯慧. 应用流式细胞术测定18个中国古老月季基因组大小[J]. 北京林业大学学报, 2016, 38(6): 94-100. doi: 10.13332/j.1000-1522.20150364
WU Rong-hua, GE Bei-bei, WANG Mao-liang, ZHOU Yan, FENG Hui. Estimation of genome size of eighteen Chinese old garden roses by flow cytometry[J]. Journal of Beijing Forestry University, 2016, 38(6): 94-100. doi: 10.13332/j.1000-1522.20150364
Citation: WU Rong-hua, GE Bei-bei, WANG Mao-liang, ZHOU Yan, FENG Hui. Estimation of genome size of eighteen Chinese old garden roses by flow cytometry[J]. Journal of Beijing Forestry University, 2016, 38(6): 94-100. doi: 10.13332/j.1000-1522.20150364

应用流式细胞术测定18个中国古老月季基因组大小

doi: 10.13332/j.1000-1522.20150364
基金项目: 

北京市公园管理中心科技项目(ZX2015019)

详细信息
    作者简介:

    武荣花,博士,副教授。主要研究方向:园林植物育种。Email:wuronghua06@126.com 地址:450000河南省郑州市文化路95号河南农业大学林学院。

    责任作者:

    冯慧,教授级高级工程师。主要研究方向:月季引选育种。 Email: 270807944@qq.com 地址:100000北京市朝阳区花家地甲7号北京市园林科学研究院。

Estimation of genome size of eighteen Chinese old garden roses by flow cytometry

  • 摘要: 以18个中国古老月季为试材,以欧芹作为标准植物,采用2种不同的样本处理方法对其基因组大小进行测定,同时利用染色体计数法补充3个存在争议或尚未见报道的中国古老月季品种的倍性,为其基因组大小的确定提供必需的数据支持。结果表明:1)中国古老月季品种‘屏东月季'为二倍体(2n=2x=14),‘桔囊'和‘牡丹月季'为四倍体(2n=4x=28)。2)2种样本处理方法通过单因素方差分析发现,6个品种间差异显著,12个品种间差异不显著。故2种处理方法均可用于基因组大小的检测,但方法Ⅱ准确度高,受外界影响小,更适宜基因组大小的检测。方法Ⅰ操作简便,可用于大规模的倍性检测。3)18个中国古老月季品种(含二倍体、三倍体、四倍体)的基因组大小在0.62~0.71pg之间,其中二倍体品种2C DNA含量范围为1.34~1.43pg,C值范围为0.67~0.71pg;三倍体2C DNA含量范围为1.96~2.05pg,C值范围为0.65~0.68pg;四倍体2C DNA含量范围为2.49~2.63pg,C值范围为0.62~0.66pg。二倍体基因组最大,三倍体居中,四倍体最小。本研究找到了适宜的流式细胞术样本处理方法,并检测出18个中国古老月季品种的基因组大小,为揭示中国古老月季的起源与进化提供了依据,也为其之后的基因组测序工作奠定了基础。

     

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出版历程
  • 收稿日期:  2015-10-15
  • 修回日期:  2015-10-15
  • 刊出日期:  2016-06-30

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