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长白落叶松小RNA测序和其靶基因预测

张素芳 张磊 赵佳丽 张莉 张含国

张素芳, 张磊, 赵佳丽, 张莉, 张含国. 长白落叶松小RNA测序和其靶基因预测[J]. 北京林业大学学报, 2016, 38(12): 64-72. doi: 10.13332/j.1000-1522.20150404
引用本文: 张素芳, 张磊, 赵佳丽, 张莉, 张含国. 长白落叶松小RNA测序和其靶基因预测[J]. 北京林业大学学报, 2016, 38(12): 64-72. doi: 10.13332/j.1000-1522.20150404
ZHANG Su-fang, ZHANG Lei, ZHAO Jia-li, ZHANG Li, ZHANG Han-guo.. Small RNA sequencing and target gene prediction in Larix olgensis.[J]. Journal of Beijing Forestry University, 2016, 38(12): 64-72. doi: 10.13332/j.1000-1522.20150404
Citation: ZHANG Su-fang, ZHANG Lei, ZHAO Jia-li, ZHANG Li, ZHANG Han-guo.. Small RNA sequencing and target gene prediction in Larix olgensis.[J]. Journal of Beijing Forestry University, 2016, 38(12): 64-72. doi: 10.13332/j.1000-1522.20150404

长白落叶松小RNA测序和其靶基因预测

doi: 10.13332/j.1000-1522.20150404
基金项目: 

“863”国家高技术研究发展计划项目(2013AA102704)、中央高校基本科研业务费专项(2572016CA08、2572016AA36)。

详细信息
    作者简介:

    张素芳,博士生。主要研究方向:林木遗传改良。Email:171998329@qq.com 地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。
    责任作者: 张含国,教授,博士生导师。主要研究方向:林木遗传改良。Email:hanguozhang1@sina.com 地址:同上。

    张素芳,博士生。主要研究方向:林木遗传改良。Email:171998329@qq.com 地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。
    责任作者: 张含国,教授,博士生导师。主要研究方向:林木遗传改良。Email:hanguozhang1@sina.com 地址:同上。

Small RNA sequencing and target gene prediction in Larix olgensis.

  • 摘要: miRNA是近年来新发现的一种比较特殊的具有高度保守的小RNA,其在基因调控网络中扮演着重要的角色。本文借助Illumia测序平台,经过Solexa测序分析,对长白落叶松的miRNA进行了分析,最终筛选出了15 294 797条clean reads。比对结果显示,除去核糖体RNA(rRNA)2 991 972条、转运RNA(tRNA)65 264条、核内小RNA(snRNA)1 670条、核仁小RNA(snoRNA)467条等ncRNA以及重复序列6 421条,共获得未获得注释的miRNA 12 229 003条,占总注释小RNA的79.96%。利用miRDeep2对未注释的小RNA进行miRNA的筛选,共得到78个新发现的miRNA,分别隶属于miR164、miR166、miR160、miR950、miR396家族。通过进一步对靶基因的注释分析共得到333个与其相对应的靶基因;其功能包括转录因子类,未知功能蛋白,离子结合蛋白,延长因子,信号转导,细胞壁或细胞膜的生物合成等调控植物生长发育过程。

     

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出版历程
  • 收稿日期:  2015-12-19
  • 刊出日期:  2016-12-31

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