Ultrastructural investigations of Paeonia pollen activation and pollen tube growth after intersectional hybridization.
-
摘要: 以探明牡丹与芍药远缘杂交结实率低的原因为目标,以芍药‘朱砂判’为母本,以牡丹‘凤丹白’、‘岛大臣’、‘正午’、‘姚黄’为父本,并以芍药品种‘Kansas’为对照父本进行人工杂交,采用培养液培养法测定父本花粉萌发率,用苯胺蓝染色法,在荧光显微镜下观察父本花粉在母本柱头上萌发及花粉管生长的过程。结果显示:‘朱砂判’在开花第4天为最佳授粉期;离体状态下花粉萌发率由高到低为‘Kansas’、‘岛大臣’、‘凤丹白’、‘姚黄’、‘正午’;‘岛大臣’及‘Kansas’作父本的组合花粉管能够到达子房,其他组合花粉管都未到达子房,并观察到一些异常的花粉管形态,花粉管生长过程中还伴随着一系列胼胝质反应。表明受精前障碍是影响牡丹与芍药组间远缘杂交的主要原因之一。Abstract: In order to reveal the causes of low seed setting rate in the intersectional cross between herbaceous and tree peonies, hybridization was conducted between Paeonia lactiflora ‘Zhu Sha Pan’ and four tree peony cultivars ‘Feng Dan Bai’, ‘Dao Da Chen’, ‘High Noon’, and ‘Yao Huang’. Herbaceous peony cultivar ‘Kansas’ was taken as a control pollen parent. Pollen viabilities were tested in vitro. With aniline blue staining, the germinating rate of pollen grain and the growth process of pollen tubes in stigma were determined under fluorescent microscope. The results showed that the best pollination period of ‘Zhu Sha Pan’ was 4 d after blooming; pollen germination rate from high to low was ‘Kansas’, ‘Dao Da Chen’, ‘Feng Dan Bai’, ‘Yao Huang’, ‘High Noon’. Only pollen tubes from ‘Dao Da Chen’ and ‘Kansas’ could arrive at the ovary. In the growth process of pollen tubes, we also observed some unusual pollen tube morphology accompanied with a series of callose reactions. In conclusion, the incompatible reaction caused by pollen-stigma interaction could be one of the main barriers of intersectional crossing between Sect. Moutan and Sect. Paeonia.
-
Keywords:
- tree peony /
- herbaceous peony /
- distant hybridization /
- pollen tube growth
-
-
[1] LI J J. Peonies of China [M]. Beijing: China Forestry Publishing House, 1995.
[1] 李嘉珏.中国牡丹与芍药[M].北京: 中国林业出版社, 1995. [2] 王历慧, 郑黎文, 于晓南. 中西方芍药切花应用与市场趋势分析[J]. 黑龙江农业科学, 2011(2): 147-149. [2] WANG L H, ZHENG L W, YU X N. Studies on the application and flora market trend of cut peonies in China and overseas [J]. Heilongjiang Agricultural Sciences, 2011(2): 147-149.
[3] 于晓南, 季丽静, 王琪. 芍药属植物分子水平遗传多样性研究进展[J]. 北京林业大学学报, 2012, 34(3): 130-136. [3] YU X N, JI L J, WANG Q. Research advances in molecular genetic diversity of Paeonia L. [J]. Journal of Beijing Forestry University, 2012, 34(3): 130-136.
[4] 戴华军, 朱正斌, 沈雪林, 等. 作物远缘杂交育种的途径及其实质[J]. 基因组学与应用生物学, 2010, 29(1): 144-149. [4] DAI H J, ZHU Z B, SHEN X L, et al. Essences and approaches of distant hybridization in crops breeding [J]. Genomics and Applied Biology, 2010, 29(1): 144-149.
[5] 张美玲, 陈洪伟, 王红利, 等. 植物远缘杂交育种现状与展望[J]. 安徽农业科学, 2015, 43(1): 11-17. [5] ZHANG M L, CHEN H W, WANG H L, et al. Status and prospect of plants’ distant hybridization breeding [J]. Journal of Anhui Agriculture Sciences, 2015, 43(1): 11-17.
[6] HOU X Y, GUO X F. Progress in Paeonia crossbreeding research [J]. Acta Horticulturae Sinica, 2013, 40(9): 1805-1812.
[6] 侯祥云, 郭先锋. 芍药属植物杂交育种研究进展[J]. 园艺学报, 2013, 40(9): 1805-1812. [7] LU G P, YU X N. An analysis of the American Peony Society’s gold medal [J]. Journal of Central South University of Forestry Technology, 2009, 29(5): 191-194.
[7] ROGERS A. Peonies [M]. Portland: Timber Press, 1995.
[8] JING D D, LIU Z A, LI X X, et al. Preliminary study on fertility of a distant hybrid ‘Hexie’ of tree peony [J]. Scientia Silvae Sinicae, 2011, 47(10): 59-62.
[8] 陆光沛, 于晓南. 美国芍药牡丹协会金牌奖探析[J]. 中南林业科技大学学报, 2009, 29(5): 191-194. [9] 荆丹丹, 刘政安, 李新旭, 等. 牡丹远缘杂种‘和谐’育性的初步研究[J]. 林业科学, 2011, 47(10): 59-62. [9] LI X L, CHEN F D, ZHAO H B. Compatibility of interspecific cross in Dendranthema genus [J]. Acta Horticulturae Sinaca, 2008, 35(2): 257-262.
[10] SUN C Q, CHEN F D, FANG W M, et al. Pollen viability, pistil receptivity and embryogenesis in the cross between Dendranthema indicum and D. grandiflorum [J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(7): 1335- 1341.
[10] 李辛雷,陈发棣, 赵宏波. 菊属植物远缘杂交亲和性研究[J]. 园艺学报, 2008, 35(2): 257-262. [11] GAO X, CAO Q Z, HUANG F J, et al. Compatibility for lily distant hybridization between Asiatic hybrids and oriental hybrids [J]. Journal of Northeast for Forestry University, 2015, 43(3): 1-5.
[11] 孙春青, 陈发棣, 房伟民, 等.野菊与菊花杂交中花粉活力和柱头可授性及胚胎发育研究[J]. 西北植物学报, 2009, 29(7): 1335-1341. [12] 高雪, 曹钦政, 黄凤杰, 等. 亚洲百合与东方百合远缘杂交亲和性[J]. 东北林业大学学报, 2015, 43(3): 1-5. [12] LEI J J, WU C. Fluorescence observation of pollen tube growth from interspecific hybridization between Lilium lancifolium Thumb and Asiatic lilies[J]. Journal of Northeast Agricultural University, 2012, 43(4): 117-120.
[13] 雷家军, 吴超. 卷丹与亚洲百合种间杂交花粉管生长的荧光观察[J]. 东北农业大学学报, 2012, 43(4): 117-120. [13] LIU C, MU D, MING J, et al. The pre-fertilization barriers research of Lilium interspecific hybridization [J]. Acta Horticulturae Sinaca, 2006, 33(3): 653-656.
[14] 刘春, 穆鼎, 明军,等. 百合种间杂交受精前障碍的研究[J]. 园艺学报, 2006, 33(3): 653-656. [14] WANG W H, WANG S D, ZHAO X Y, et al. Process of pollen sprouting and pollen tube growth on the lily distant hybridization [J]. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(9): 1790-1794.
[15] 王文和, 王树栋, 赵祥云, 等. 百合远缘杂交花粉萌发及花粉管生长过程观察[J]. 西北植物学报, 2007, 27(9): 1790-1794. [15] LI S L, SHI L, ZHANG J Z. Pollen tube behavior following pollination between Cardiocrinum giganteum and Lilium [J]. Acta Horticulturae Sinaca, 2006, 33(6): 1259-1262.
[16] DU W W, WANG X N, WU L F, et al. Difference analysis on the degree of positive and negative hybridization affinity for Asian lily and longiflorum lily [J]. Scientia Agricultura Sinica, 2012, 45(23): 4854-4861.
[16] 李守丽, 石雷, 张金政.大百合与百合属授粉后花粉管生长发育的观察[J]. 园艺学报, 2006, 33(6): 1259 – 1262. [17] CAI M, MENG R, PAN H T, et al. Intergeneric cross-compatibility between Lagerstroemia and Lawsonia [J]. Acta Horticulturae Sinaca, 2010, 37(4): 637-642.
[17] 杜文文, 王祥宁, 吴丽芳, 等. 亚洲百合和铁炮百合正反杂交亲和程度的差异性分析[J]. 中国农业科学, 2012, 45(23): 4854-4861. [18] CAI M, WANG X Y, ZHANG Q X, et al. Compatibility of interspecific crosses between Lagerstroemia indica cultivars and Lagerstroemia caudata [J]. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(4): 645-651.
[18] 蔡明, 孟锐, 潘会堂, 等.紫薇属与散沫花属远缘杂交亲和性的研究[J]. 园艺学报, 2010, 37(4): 637-642. [19] L C Y, WANG Y, ZHU X T, et al. Anatomical studies on pollen-stigma interaction in intersectional cross of Paeonia lutea and Paeonia lactiflora [J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(10): 1988-1994.
[19] 蔡明, 王晓玉, 张启翔, 等. 紫薇品种与尾叶紫薇种间杂交亲和性研究[J]. 西北植物学报, 2010, 30(4): 645-651. [20] HAO J L, ZHAO N, SHI Y T, et al. Distant hybridization compatibility of Paeonia lutea and morphological analysis of its progenies [J]. Acta Horticulturae Sinaca, 2014, 41(8): 1651-1662.
[20] KULIGOWSKA K, LTKEN H, CHRISTENSEN B, et al. Evaluation of reproductive barriers contributes to the development of novel interspecific hybrids in the Kalancho genus[J]. BMC Plant Biology, 2015, 15(1):15-15.
[21] HE D, WANG X L, GAO X F, et al. Intergeneric cross-compatibility between peonies [J]. Journal of Northeast for Forestry University, 2014, 42(7): 65-68.
[21] 律春燕, 王雁, 朱向涛, 等. 黄牡丹与芍药组间杂交花粉与柱头识别的解剖学研究[J]. 西北植物学报, 2009, 29(10): 1988-1994. [22] 郝津藜, 赵娜, 石颜通, 等. 黄牡丹远缘杂交亲和性及杂交后代形态分析[J]. 园艺学报, 2014, 41(8): 1651-1662. [22] TAO Q B. Studies on cryopreservation of tree peony pollen [D]. Beijing: Beijing Forestry University, 2003.
[23] HONG Y, LIU Q, HAN L. Pollen vitality and stigma receptivity of Paeonia lactiflora Pall [J]. Guihaia, 2003, 23(1): 90-92.
[23] HAO Q, AOKI N, KATAYAMA J, et al. Crossability of American tree peony ‘High Noon’ as seed parent with Japanese cultivars to breed superior cultivars[J]. Euphytica, 2013, 191(1): 35-44.
[24] 贺丹, 王雪玲, 高晓峰, 等. 牡丹芍药远缘杂交亲和性[J]. 东北林业大学学报, 2014, 42(7): 65-68. [24] HE G M, CHENG F Y. Morphological observation of sexual reproduction abortion in ‘High Noon’ tree peony [J]. Acta Horticulturae Sinaca, 2006, 33(3): 660-663.
[25] 陶清波. 牡丹花粉超低温保存研究[D]. 北京: 北京林业大学, 2003. [25] YU X Y, XING S T, ZHAO L Y. Analysis on the barriers of interspecific hybridization between Rosa rugosa and Rosa hybrid [J]. Scientia Agricultura Sinica, 2014, 47(15): 3112-3120.
[26] 红雨, 刘强, 韩岚. 芍药花粉活力和柱头可授性的研究[J]. 广西植物, 2003, 23(1): 90-92. [26] HU H F, ZHANG Y L, GUO W Z, et al. Cross compatibility in the interspecies cross between some sections in genus Camellia [J] Acta Agriculturae Universitatis Jiangxiensis, 2014, 36(2): 338-343.
[27] 何桂梅, 成仿云. ‘正午’牡丹有性生殖败育的形态学观察[J]. 园艺学报, 2006, 33(3): 660-663. [27] TANG X L, LIU Y M, PAN H T, et al. Intergeneric cross-compatibility between Primula forbesii(Section Monocarpicae) and Primula saxatilis(Section Cortusoides) [J]. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(2): 270-275.
[28] XU J Q, SUN Z J, SONG M, et al. Studies on the interactions between the pollen tube calmodulin(CaM) and SRK from Brassica oleracea var. capitata [J]. Acta Horticulturae Sinaca, 2013, 40(12): 2429-2440.
[28] EDLUND A F, SWANSON R, PREUSS D. Pollen and stigma structure and function: the role of diversity in pollination[J]. Plant Cell, 2004, 16: 84-97.
[29] JIANG L, YANG S, XIE L, et al. VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract[J]. Plant Cell, 2005, 17: 584-596.
[29] YOU H L, JIA T T, TIAN J, et al. Characteristics of in vitro pollen germination and microfilament distribution of pollen tubes of Eucommia ulmoides oliv [J]. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(3): 495-501.
[30] WANG C L, ZHANG S L. Application of fluorescent indicator in the study on the structure and growth characteristics of pollen tubes [J]. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(2): 407-413.
[30] 于晓艳, 邢树堂, 赵兰勇. 玫瑰与月季种间杂交障碍原因分析. 中国农业科学, 2014, 47(15): 3112-3120. [31] 胡禾丰, 张亚利, 郭卫珍, 等. 山茶属部分组间远缘杂交亲和性初探[J]. 江西农业大学学报, 2014, 36(2): 338-343. [32] 唐星林, 刘艳梅, 潘会堂, 等. 小报春与岩生报春种间杂交亲和性研究[J]. 西北植物学报, 2014, 34(2): 270-275. [33] HISCOCK S J, ALLEN A M. Diverse cell signaling pathways regulate pollen-stigma interaction: the search for consensus[J]. New Phytol, 2008, 179: 286-317.
[34] 许俊强, 孙梓健, 宋明, 等. 甘蓝花粉管钙感应蛋白CaM与SRK相互作用研究[J]. 园艺学报, 2013, 40(12): 2429-2440. [35] 游韩莉, 贾婷婷, 田菊, 等. 杜仲花粉离体萌发特征及花粉管微丝骨架分布[J]. 西北植物学报, 2014, 34(3): 495-501. [36] 王春雷, 张绍铃. 荧光标记在植物花粉管构造及生长特性研究中的应用[J]. 西北植物学报, 2007, 27(2): 407-413. -
期刊类型引用(8)
1. 蒋至立，耿兴敏，祝遵凌，圣倩倩. 牡丹杂交育种研究进展. 分子植物育种. 2023(02): 602-619 . 
百度学术
2. 刘玉菡，陶宁，王庆国，李清清. 番茄中ABC转运蛋白SlABCG23调控茉莉酸信号途径. 园艺学报. 2023(03): 559-568 . 百度学术
3. 贺丹，尤啸龙，何松林，张明星，张佼蕊，华超，王政，刘艺平. 芍药胼胝质合成酶基因家族鉴定及PlCalS5功能分析. 中国农业科学. 2023(16): 3183-3198 . 百度学术
4. 袁雪，唐英，陈庭巧，郝津藜，袁涛. 紫牡丹杂交结实率的差异. 分子植物育种. 2021(17): 5782-5792 . 百度学术
5. 祁奇墨，孟凡志，国静，邢广萍，郭先锋. 4个芍药品种与3个牡丹品种的远缘杂交结实特性研究. 山东农业科学. 2020(03): 24-28 . 百度学术
6. 柴弋霞，胡希军，张冬林，刘晓玲，刘彩贤，金晓玲. 紫花含笑与含笑、深山含笑和阔瓣含笑杂交亲和性分析. 园艺学报. 2018(10): 1970-1978 . 百度学术
7. 刘改秀. 牡丹与芍药远缘杂交育种技术. 现代农业科技. 2017(02): 125-126 . 百度学术
8. 苏云凤，岳中辉. 被子植物花粉与柱头间信息传递研究进展. 生物学教学. 2017(07): 4-5 . 百度学术
其他类型引用(8)
计量
- 文章访问数: 2600
- HTML全文浏览量: 237
- PDF下载量: 43
- 被引次数: 16
下载:
