Extraction and purification conditions of anthocyanin from the fruit of Aronia melanocarpa.
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摘要: 以黑果花楸花色苷得率为考察指标,分别用水,50%、60%、70%、80%、90%、100%的乙醇和50%、60%、70%、80%、90%、100%的甲醇作为溶剂提取黑果花楸花色苷,得出80%乙醇为较好的提取溶剂。以80%乙醇为提取溶剂,进行提取时间、料液比、pH值、提取温度4个单因素试验。在单因素试验基础上,运用Box-Behnken响应面设计建立数学模型。结果表明最佳提取条件为:提取时间110 min、pH值2、提取温度34 ℃、料液比1∶16.5,黑果花楸花色苷得率为6.648 mg/g。通过静态吸附和解吸筛选出黑果花楸花色苷的最佳纯化树脂为AB-8,最佳纯化条件为上样质量浓度2 mg/mL,径长比1∶25,用2.2 BV、pH值2的80%乙醇以2.0 BV/h的流速洗脱,可使花色苷纯度提高11.5倍。Abstract: Anthocyanins were extracted from Aronia melanocarpa with water, 50%-100% ethanol and 50%-100% methanol. The results showed that the optimal yield of anthocyanins was with 80% ethanol. Four single factor experiments were designed for extracting time, solid-liquid ratio, pH and temperature. Based on the single factor experiment analysis, the mathematical model was established by Box-Behnken response surface design. The results showed that the optimum extracting conditions were: extracting time 110 min, pH 2, extracting temperature 34 ℃, solid-liquid ratio 1∶16.5. Under these conditions, anthocyanins yield reached 6.648 mg/g. AB-8 was chosen as the optimal resin after the static adsorption and desorption testing. The optimum purification conditions were 2 mg/mL of solution concentration, 1∶25 of diameter to length, 2.2 BV, pH 2 and 80% ethanol with the elution flow rate of 2.0 BV/h, the anthocyanins purity increased 11.5 times.
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