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黑果花楸花色苷提取、纯化工艺研究

郑悦 王萍

郑悦, 王萍. 黑果花楸花色苷提取、纯化工艺研究[J]. 北京林业大学学报, 2016, 38(11): 118-124. doi: 10.13332/j.1000-1522.20150484
引用本文: 郑悦, 王萍. 黑果花楸花色苷提取、纯化工艺研究[J]. 北京林业大学学报, 2016, 38(11): 118-124. doi: 10.13332/j.1000-1522.20150484
ZHENG Yue, WANG Ping.. Extraction and purification conditions of anthocyanin from the fruit of Aronia melanocarpa.[J]. Journal of Beijing Forestry University, 2016, 38(11): 118-124. doi: 10.13332/j.1000-1522.20150484
Citation: ZHENG Yue, WANG Ping.. Extraction and purification conditions of anthocyanin from the fruit of Aronia melanocarpa.[J]. Journal of Beijing Forestry University, 2016, 38(11): 118-124. doi: 10.13332/j.1000-1522.20150484

黑果花楸花色苷提取、纯化工艺研究

doi: 10.13332/j.1000-1522.20150484
基金项目: 

卓越农林人才教育培养计划改革试点项目(41110211)。

详细信息
    作者简介:

    郑悦。主要研究方向:天然产物分离。Email: 1484735552@qq.com 地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。   责任作者: 王萍,教授。主要研究方向:天然产物分离。Email: 949508700@qq.com 地址:同上。

    郑悦。主要研究方向:天然产物分离。Email: 1484735552@qq.com 地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。   责任作者: 王萍,教授。主要研究方向:天然产物分离。Email: 949508700@qq.com 地址:同上。

Extraction and purification conditions of anthocyanin from the fruit of Aronia melanocarpa.

  • 摘要: 以黑果花楸花色苷得率为考察指标,分别用水,50%、60%、70%、80%、90%、100%的乙醇和50%、60%、70%、80%、90%、100%的甲醇作为溶剂提取黑果花楸花色苷,得出80%乙醇为较好的提取溶剂。以80%乙醇为提取溶剂,进行提取时间、料液比、pH值、提取温度4个单因素试验。在单因素试验基础上,运用Box-Behnken响应面设计建立数学模型。结果表明最佳提取条件为:提取时间110 min、pH值2、提取温度34 ℃、料液比1∶16.5,黑果花楸花色苷得率为6.648 mg/g。通过静态吸附和解吸筛选出黑果花楸花色苷的最佳纯化树脂为AB-8,最佳纯化条件为上样质量浓度2 mg/mL,径长比1∶25,用2.2 BV、pH值2的80%乙醇以2.0 BV/h的流速洗脱,可使花色苷纯度提高11.5倍。

     

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出版历程
  • 收稿日期:  2015-12-11
  • 刊出日期:  2016-11-30

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