Growth and physiological responses to drought stress and comprehensive evaluation on drought tolerance in Leuce clones at nursery stage
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摘要: 为揭示白杨派无性系对干旱环境的适应能力,筛选速生耐旱优良无性系,本文采用盆栽试验方法,通过土壤自然干旱处理,比较分析了土壤含水量分别占田间持水量80%以上(对照)、50%~70%(轻度干旱)、30%~50%(中度干旱)以及低于30%(重度干旱)等不同土壤干旱程度对9个白杨派无性系生长及生理代谢影响。结果表明:9个无性系之间的苗高增长量、地径增长量、生物量增量和根冠比差异均显著,其中无性系89和YX2表现出较强的速生性。叶绿素含量在轻度干旱胁迫时上升,中度和重度干旱胁迫下持续下降,其中下降最小的为无性系89和YX2。随干旱胁迫程度增加,9个无性系叶片脯氨酸和可溶性糖含量持续增加,其中无性系Z3、YX2和89显著高于相同干旱条件下其他无性系。干旱胁迫导致叶片细胞膜相对透性以及丙二醛(MDA)含量提高,但相同胁迫条件下,无性系Z3、89和YX2细胞膜相对透性以及MDA含量均显著低于其他无性系。超氧化物岐化酶(SOD)活性在轻度和中度干旱胁迫下逐渐增大,在重度干旱胁迫下减小,其中无性系89和YX2的SOD活性始终高于对照。进一步利用主成分分析和隶属函数法对9个无性系生长及生理代谢等参数进行综合评价,各无性系抗旱性由强到弱依次为89、YX2、LM50、Z3、Y3、150、27、26、1316。因此,初步认为无性系89和YX2具有显著耐旱性和速生性,可作为干旱立地造林或抗逆育种的重要试验材料。Abstract: In order to explore the responses of white poplar clones to drought stress and select superior clones with characteristics of fast growth and strong drought resistance, the growth and physiological characteristics of nine white poplar clones grown in pots under different drought stresses were investigated. The soil moisture content was greater than 80% of field capacity for the normal level (control), 50%-70% for mild drought, 30%-50% for moderate drought, and less than 30% for severe drought. The results showed that the increment of height, basal diameter and biomass, and root-shoot ratio of clones had significant differences among the nine clones. Two clones, 89 and YX2, showed fast growth. The chlorophyll content increased under mild drought stress,but then decreased rapidly under moderate and severe drought stress, and the chlorophyll content of clone 89 and YX2 showed the smallest decline under drought stress. The contents of the free proline and the soluble sugar in nine clones showed the trend of increasing gradually with the development of soil drought, and that of clone Z3, YX2 and 89 was significantly higher than the other clones. The cell membrane permeability and MDA content had a steady increase with the soil water content gradually reducing, and that of clone Z3,YX2 and 89 was significantly lower than others under the same drought condition. The activities of SOD increased under mild and moderate drought stress, and then decreased under severe drought stress. The activities of SOD in the clone 89 and YX2 were always higher than that in control. Comprehensive assessments of drought resistance based on the principal component analysis and subordinate function values analysis indicated that the sequence of drought resistance in nine clones from high to low was as follows: 89, YX2, LM50, Z3, Y3, 150, 27, 26, and 1316. Therefore, it was preliminarily considered that the clone 89 and YX2 had significant drought resistance and fast growth, which could be used as the important materials for afforestation in arid area and breeding resource in stress resistance research.
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Keywords:
- Leuce /
- drought stress /
- growth /
- physiological index /
- drought resistance
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