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超表达杨树RPEase基因促进了拟南芥的生长发育

王丹 安轶 韩潇 周扬颜 王厚领 郭惠红 夏新莉 尹伟伦

王丹, 安轶, 韩潇, 周扬颜, 王厚领, 郭惠红, 夏新莉, 尹伟伦. 超表达杨树RPEase基因促进了拟南芥的生长发育[J]. 北京林业大学学报, 2016, 38(5): 67-76. doi: 10.13332/j.1000-1522.20150507
引用本文: 王丹, 安轶, 韩潇, 周扬颜, 王厚领, 郭惠红, 夏新莉, 尹伟伦. 超表达杨树RPEase基因促进了拟南芥的生长发育[J]. 北京林业大学学报, 2016, 38(5): 67-76. doi: 10.13332/j.1000-1522.20150507
WANG Dan, AN Yi, HAN Xiao, ZHOU Yang-yan, WANG Hou-ling, GUO Hui-hong, XIA Xin-li, YIN Wei-lun. Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2016, 38(5): 67-76. doi: 10.13332/j.1000-1522.20150507
Citation: WANG Dan, AN Yi, HAN Xiao, ZHOU Yang-yan, WANG Hou-ling, GUO Hui-hong, XIA Xin-li, YIN Wei-lun. Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2016, 38(5): 67-76. doi: 10.13332/j.1000-1522.20150507

超表达杨树RPEase基因促进了拟南芥的生长发育

doi: 10.13332/j.1000-1522.20150507
基金项目: 

十二五”

国家科技支撑计划课题(2015BAD07B01)、国家自然科学基金项目 (31270656)、北京市科学研究与研究生培养共建项目

详细信息
    作者简介:

    王丹。主要研究方向:植物抗逆分子生物学。Email : summer1045@163.com 地址:100083 北京市清华东路35号北京林业大学林木育种国家工程实验室。

    责任作者:

    郭惠红,副教授。主要研究方向:植物发育生物学。Email: guohh@bjfu.edu.cn 地址:同上。夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email: xiaxl@bjfu.edu.cn 地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email: yinwl@bjfu.edu.cn 地址:同上。

    郭惠红,副教授。主要研究方向:植物发育生物学。Email: guohh@bjfu.edu.cn 地址:同上。夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email: xiaxl@bjfu.edu.cn 地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email: yinwl@bjfu.edu.cn 地址:同上。

    郭惠红,副教授。主要研究方向:植物发育生物学。Email: guohh@bjfu.edu.cn 地址:同上。夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email: xiaxl@bjfu.edu.cn 地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email: yinwl@bjfu.edu.cn 地址:同上。

Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana

  • 摘要: 卡尔文循环是光合作用CO2同化的重要途径,在植物生长发育过程中起着重要作用。磷酸核酮糖3-差向异构酶(RPEase:EC 5.1.3.1)是卡尔文循环再生阶段的一种重要酶类。本文从速生欧美杂交黑杨NE-19中克隆得到RPEase基因,构建植物表达载体,利用农杆菌花序侵染法转化野生型和突变体拟南芥,通过普通PCR检测和绿色荧光蛋白(GFP)观测进一步鉴定得到CaMV35S:PdRPE:GFP超表达株系,然后对野生型、超表达株系、突变体和回补株系的生理指标进行测定。结果显示,超表达株系RPEase活性显著升高(P<0.05)。在正常浇水的生长条件下,超表达株系相比于其他3个株系(野生型、突变体、回补株系),气孔数目减少,气孔变大,提高了植物的水分利用效率以及净光合速率,使得超表达株系有更好的生长优势,积累更多的淀粉。在10d的短期干旱条件下,超表达株系的净光合速率和水分利用效率依然显著高于其他3个株系(P<0.01)。因此,研究表明超表达RPEase基因会提高植物生物量的积累以及对短期干旱的抵抗能力。

     

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  • 收稿日期:  2015-12-28
  • 修回日期:  2015-12-28
  • 刊出日期:  2016-05-31

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