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毛果杨基因PtNRT2.7的功能初步鉴定与分析

唐贤礼 张月 张盾 夏新莉 尹伟伦

唐贤礼, 张月, 张盾, 夏新莉, 尹伟伦. 毛果杨基因PtNRT2.7的功能初步鉴定与分析[J]. 北京林业大学学报, 2016, 38(8): 18-27. doi: 10.13332/j.1000-1522.20160041
引用本文: 唐贤礼, 张月, 张盾, 夏新莉, 尹伟伦. 毛果杨基因PtNRT2.7的功能初步鉴定与分析[J]. 北京林业大学学报, 2016, 38(8): 18-27. doi: 10.13332/j.1000-1522.20160041
TANG Xian-li, ZHANG Yue, ZHANG Dun, XIA Xin-li, YIN Wei-lun.. Functional identification and analysis of PtNRT2.7 gene from Populus trichocarpa.[J]. Journal of Beijing Forestry University, 2016, 38(8): 18-27. doi: 10.13332/j.1000-1522.20160041
Citation: TANG Xian-li, ZHANG Yue, ZHANG Dun, XIA Xin-li, YIN Wei-lun.. Functional identification and analysis of PtNRT2.7 gene from Populus trichocarpa.[J]. Journal of Beijing Forestry University, 2016, 38(8): 18-27. doi: 10.13332/j.1000-1522.20160041

毛果杨基因PtNRT2.7的功能初步鉴定与分析

doi: 10.13332/j.1000-1522.20160041
基金项目: 

林业公益性行业科研专项(201304301)、国家自然科学基金项目(31270656)和高等学校学科创新引智计划项目(111 Project、B13007)。

详细信息
    作者简介:

    唐贤礼。主要研究方向:植物逆境生理及其分子机制。Email:txianli@163.com地址:100083北京市海淀区清华东路35号北京林业大学林木育种国家工程实验室。责任作者:夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email:xiaxl@bjfu.edu.cn地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email:yinwl@bjfu.edu.cn地址:同上。

    唐贤礼。主要研究方向:植物逆境生理及其分子机制。Email:txianli@163.com地址:100083北京市海淀区清华东路35号北京林业大学林木育种国家工程实验室。责任作者:夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email:xiaxl@bjfu.edu.cn地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email:yinwl@bjfu.edu.cn地址:同上。

    唐贤礼。主要研究方向:植物逆境生理及其分子机制。Email:txianli@163.com地址:100083北京市海淀区清华东路35号北京林业大学林木育种国家工程实验室。责任作者:夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email:xiaxl@bjfu.edu.cn地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email:yinwl@bjfu.edu.cn地址:同上。

Functional identification and analysis of PtNRT2.7 gene from Populus trichocarpa.

  • 摘要: 本研究从毛果杨基因组中克隆一个NRT2家族的新基因PtNRT2.7。生物信息学分析显示,其编码的蛋白不存在信号肽及切割位点,属于非分泌蛋白;具有11个跨膜结构域,亲水区与输水区交替分布,主要分布于细胞膜,而且该基因的亚细胞定位分析显示该蛋白定位于细胞膜,综合以上结果可判断此蛋白为膜蛋白。对其在毛果杨的表达特征分析显示,PtNRT2.7基因主要在叶片中表达,其中成熟叶中的表达量最高;PtNRT2.7基因受硝酸盐诱导并在1 h时达到表达峰值;SA、JA、GA或IAA激素处理可诱导PtNRT2.7基因表达,而在Eth、ABA或NaCl处理下该基因的表达受抑制。利用农杆菌花序侵染法转化野生型拟南芥和突变体(nrt2.7),显示在KNO3浓度大于0.1 mmol/L时,PtNRT2.7过表达株系相比较拟南芥野生型和突变体株系促进了根的生长,而atnrt2.7突变体在KNO3浓度大于1 mmol/L的条件下抑制了根的生长,且PtNRT2.7过表达atnrt2.7突变体株系可恢复正常生长。研究结果表明,PtNRT2.7可以由硝酸盐和激素诱导并增强植物根系的生长,提高氮素的吸收利用。

     

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出版历程
  • 收稿日期:  2016-03-01
  • 刊出日期:  2016-08-31

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