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干旱对欧美杨气孔发育的影响

王丛鹏 贾伏丽 刘沙 刘超 夏新莉 尹伟伦

王丛鹏, 贾伏丽, 刘沙, 刘超, 夏新莉, 尹伟伦. 干旱对欧美杨气孔发育的影响[J]. 北京林业大学学报, 2016, 38(6): 28-34. doi: 10.13332/j.1000-1522.20160050
引用本文: 王丛鹏, 贾伏丽, 刘沙, 刘超, 夏新莉, 尹伟伦. 干旱对欧美杨气孔发育的影响[J]. 北京林业大学学报, 2016, 38(6): 28-34. doi: 10.13332/j.1000-1522.20160050
WANG Cong-peng, JIA Fu-li, LIU Sha, LIU Chao, XIA Xin-li, YIN Wei-lun. Drought induces alterations in stomatal development in Populus deltoides×P. nigra[J]. Journal of Beijing Forestry University, 2016, 38(6): 28-34. doi: 10.13332/j.1000-1522.20160050
Citation: WANG Cong-peng, JIA Fu-li, LIU Sha, LIU Chao, XIA Xin-li, YIN Wei-lun. Drought induces alterations in stomatal development in Populus deltoides×P. nigra[J]. Journal of Beijing Forestry University, 2016, 38(6): 28-34. doi: 10.13332/j.1000-1522.20160050

干旱对欧美杨气孔发育的影响

doi: 10.13332/j.1000-1522.20160050
基金项目: 

十二五”

国家科技支撑计划项目(2015BAD07B01)、国家自然科学基金项目(31270656)

详细信息
    作者简介:

    王丛鹏,博士生。主要研究方向: 植物抗逆分子生物学。Email: wangcongpeng@aliyun.com 地址:100083 北京市海淀区清华东路35号北京林业大学林木育种国家工程实验室。

    责任作者:

    夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email: xiaxl@bjfu.edu.cn 地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email: yinwl@bjfu.edu.cn 地址:同上。

    夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email: xiaxl@bjfu.edu.cn 地址:同上。尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email: yinwl@bjfu.edu.cn 地址:同上。

Drought induces alterations in stomatal development in Populus deltoides×P. nigra

  • 摘要: 为了探究干旱对木本植物气孔发育的影响,本文挑选了5个欧美杨无性系NE-19、R270、107、109和111,进行了14d的自然干旱处理及7d的复水处理。结果发现:NE-19、R270与107的光合速率在复水后第5天恢复至处理前水平,气孔导度在第3天恢复至处理前水平,而109与111在复水处理期间光合与气孔导度恢复缓慢。测量各无性系复水后的生长速率,与对照组相比由快到慢依次为NE-19(64.96%)、R270(55.73%)、107(49.87%)、109(35.08%)、111(23.62%)。综合光合、气孔导度与生长的数据,5个无性系耐旱性由强至弱的排序为NE-19、R270、107、109、111。统计5个无性系在处理期间幼叶气孔指数的变化,发现各无性系在干旱期间气孔发育速度均下降。其中NE-19与R270下降最多,分别为30.75%和29.24%,说明耐旱性好的无性系,更善于调节幼叶中的气孔发育来适应外界的水分变化。通过荧光定量PCR检测与气孔发育相关基因ERECTA、EPF1、EPFL9、FAMA、SDD1在处理期间处理组与对照组的表达量差异,发现在干旱期间对气孔发育正调控的EPFL9与FAMA表达量下降,而负调控气孔发育的ERECTA与EPF1表达量上调,在NE-19与R270中的变化幅度尤为明显,而SDD1在整个处理期间表达量没有十分明显的变化。研究发现欧美杨在干旱中可通过调节气孔发育相关基因ERECTA、EPF1、EPFL9和FAMA的表达来抑制幼叶气孔发育,从而减少叶片水分散失以抵御干旱胁迫。

     

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出版历程
  • 收稿日期:  2016-02-18
  • 修回日期:  2016-02-18
  • 刊出日期:  2016-06-30

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