Abstract: In order to study the validity of the object-based identification of tree species with high spatial resolution remote sensing image (QuickBird) and multi spectral remote sensing image (Landsat 8 OLI) coordinated, based on QuickBird high spatial resolution (panchromatic 0.61 m) remote sensing image and Landsat 8 OLI(30 m) remote sensing image, we used 2 segmentation schemes (segmentation based on QuickBird remote sensing image with Landsat 8 OLI remote sensing image as an auxiliary or not) to do multi-scale segmentation, and compared the 2 segmentation schemes in the classification processing. This research applied nearest neighbor classification and support vector machine object-based classification methods, the same classification system, the unified segmentation scale and the same set of validation samples to classify tree species with 68 classification features in terms of spectral, texture and spatial extracted by QuickBird remote sensing image and Landsat 8 OLI remote sensing image, and then take use of Kappa coefficient, total accuracy, producer accuracy and user accuracy to evaluate the accuracy. The results showed that the segmentation result based on QuickBird high spatial resolution remote sensing image only was better than that based on QuickBird high spatial resolution remote sensing image and Landsat 8 OLI remote sensing image coordinated. The best segmentation threshold was 25 and the best merging threshold was 90. On the basis of the best segmentation threshold, applying Landsat 8 OLI multi spectral remote sensing image and QuickBird high spatial resolution remote sensing image together to take the object-based classification, the total accuracy of nearest neighbor classification method and support vector machine classification method was 85.35% (Kappa=0.701 3) and 88.12% (Kappa=0.853 6). And the total accuracy of the above two methods was 79.67% (Kappa=0.693 9) and 83.33% (Kappa=0.792 5) when using the QuickBird high spatial resolution remote sensing image only. Under the support of Landsat 8 OLI remote sensing image, object boundary of the classification result is clearer, the total accuracy and the accuracy of major tree species are significantly improved. The research results can effectively shorten the time and reduce the cost of investigation and survey, reduce labor intensity, improve the quality of products when it was applied in field forest survey and zoning.