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胞外ATP对胡杨细胞囊泡运输的影响

孙苑玲 邓澍荣 邓佳音 张会龙 尧俊 赵瑞 陈少良

孙苑玲, 邓澍荣, 邓佳音, 张会龙, 尧俊, 赵瑞, 陈少良. 胞外ATP对胡杨细胞囊泡运输的影响[J]. 北京林业大学学报, 2016, 38(7): 33-39. doi: 10.13332/j.1000-1522.20160071
引用本文: 孙苑玲, 邓澍荣, 邓佳音, 张会龙, 尧俊, 赵瑞, 陈少良. 胞外ATP对胡杨细胞囊泡运输的影响[J]. 北京林业大学学报, 2016, 38(7): 33-39. doi: 10.13332/j.1000-1522.20160071
SUN Yuan-ling, DENG Shu-rong, DENG Jia-yin, ZHANG Hui-long, YAO Jun, ZHAO Rui, CHEN Shao-liang.. Effect of eATP on vesicular trafficking in Populus euphratica cells.[J]. Journal of Beijing Forestry University, 2016, 38(7): 33-39. doi: 10.13332/j.1000-1522.20160071
Citation: SUN Yuan-ling, DENG Shu-rong, DENG Jia-yin, ZHANG Hui-long, YAO Jun, ZHAO Rui, CHEN Shao-liang.. Effect of eATP on vesicular trafficking in Populus euphratica cells.[J]. Journal of Beijing Forestry University, 2016, 38(7): 33-39. doi: 10.13332/j.1000-1522.20160071

胞外ATP对胡杨细胞囊泡运输的影响

doi: 10.13332/j.1000-1522.20160071
基金项目: 

国家自然科学基金项目(31270654、31570587)、教育部科学技术研究(科学技术类)项目(113013A)、人事部留学人员科技活动项目(2012001)、高等学校学科创新引智计划项目(111 Project, B13007)、教育部创新团队发展计划项目(IRT13047)。

详细信息
    作者简介:

    孙苑玲。主要研究方向:树木逆境分子与生理。Email:18003251998@163.com地址:100083北京市海淀区清华东路35号北京林业大学生物科学与技术学院。责任作者:陈少良,教授,博士生导师。主要研究方向:树木抗逆生理。Email:lschen@bjfu.edu.cn地址:同上。

    孙苑玲。主要研究方向:树木逆境分子与生理。Email:18003251998@163.com地址:100083北京市海淀区清华东路35号北京林业大学生物科学与技术学院。责任作者:陈少良,教授,博士生导师。主要研究方向:树木抗逆生理。Email:lschen@bjfu.edu.cn地址:同上。

Effect of eATP on vesicular trafficking in Populus euphratica cells.

  • 摘要: 在植物中,胞外ATP(eATP)作为一种重要的信号分子,调控植物的生长、发育及逆境响应等多种生命活动。这些植物细胞的生命活动与囊泡运输密切相关,然而,eATP是否对植物细胞的囊泡运输具有调控作用尚不清楚。本文利用能够标记囊泡运输的荧光染料FM1-43研究了eATP对胡杨细胞囊泡运输的影响。FM1-43染色结果显示,50 mol/L eATP对胡杨细胞胞吞作用不明显,而高浓度的eATP(200、400 mol/L)则会抑制其胞吞作用,其抑制作用与eATP浓度呈正相关。高浓度的eATP(200、400 mol/L)同样抑制胡杨细胞胞吐作用。而不同浓度的ADP(50、200、400 mol/L)处理则对胡杨细胞囊泡运输无明显影响。这说明高浓度eATP对胡杨细胞囊泡运输的抑制作用不是源于eATP的水解产物,而是来自于其本身的信号作用。药理学实验发现,ATP受体抑制剂PPADS(100 mol/L)能抑制高浓度eATP对胡杨细胞囊泡运输的限制作用,说明eATP是通过嘌呤受体介导的信号通路调控囊泡运输过程。但值得注意的是,嘌呤受体的另一种抑制剂suramin(100 mol/L)却对eATP的抑制作用不明显,因为suramin处理胡杨细胞后eATP(200 mol/L)仍能抑制囊泡运输。这说明在胡杨细胞中某一类与P2X同源的受体介导了高浓度eATP对囊泡运输的抑制作用。综上,eATP作为信号分子可调控胡杨细胞的囊泡运输,并且高浓度eATP对胡杨细胞的囊泡运输具有负调控作用。

     

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出版历程
  • 收稿日期:  2016-03-07
  • 刊出日期:  2016-07-30

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