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NaCl对泌盐红树和非泌盐红树Cd吸收和积累的影响

向敏, 孙会敏, 王少杰, 郎涛, 马旭君, 李妮亚, 陈少良

向敏, 孙会敏, 王少杰, 郎涛, 马旭君, 李妮亚, 陈少良. NaCl对泌盐红树和非泌盐红树Cd吸收和积累的影响[J]. 北京林业大学学报, 2016, 38(8): 10-17. DOI: 10.13332/j.1000-1522.20160079
引用本文: 向敏, 孙会敏, 王少杰, 郎涛, 马旭君, 李妮亚, 陈少良. NaCl对泌盐红树和非泌盐红树Cd吸收和积累的影响[J]. 北京林业大学学报, 2016, 38(8): 10-17. DOI: 10.13332/j.1000-1522.20160079
XIANG Min, SUN Hui-min, WANG Shao-jie, LANG Tao, MA Xu-jun, LI Ni-ya, CHEN Shao-liang. Effects of NaCl on cadmium uptake, accumulate in secretor and non-secretor mangrove species.[J]. Journal of Beijing Forestry University, 2016, 38(8): 10-17. DOI: 10.13332/j.1000-1522.20160079
Citation: XIANG Min, SUN Hui-min, WANG Shao-jie, LANG Tao, MA Xu-jun, LI Ni-ya, CHEN Shao-liang. Effects of NaCl on cadmium uptake, accumulate in secretor and non-secretor mangrove species.[J]. Journal of Beijing Forestry University, 2016, 38(8): 10-17. DOI: 10.13332/j.1000-1522.20160079

NaCl对泌盐红树和非泌盐红树Cd吸收和积累的影响

基金项目: 

国家自然科学基金项目(31270654、31160150、31570587)、教育部科学技术研究(科学技术类)项目(113013A)、人事部留学人员科技活动项目(2012001)、高等学校学科创新引智计划项目(111 Project、B13007)、教育部创新团队发展计划项目(IRT13047)。

详细信息
    作者简介:

    向敏。主要研究方向:红树逆境生理。Email:x8113462@163.com地址:571158海南海口龙昆南路99号海南师范大学生命科学学院。责任作者:李妮亚,研究员。主要研究方向:树木抗逆生理。Email:niyali6515@163.com地址:同上。

    向敏。主要研究方向:红树逆境生理。Email:x8113462@163.com地址:571158海南海口龙昆南路99号海南师范大学生命科学学院。责任作者:李妮亚,研究员。主要研究方向:树木抗逆生理。Email:niyali6515@163.com地址:同上。

Effects of NaCl on cadmium uptake, accumulate in secretor and non-secretor mangrove species.

  • 摘要: 为研究NaCl对红树植物Cd吸收和转运的影响,本文以非泌盐红树秋茄和泌盐红树桐花树幼苗为实验材料,研究了不同浓度NaCl和CdCl2处理下地上和地下各器官中Na+、Ca2+、Cd2+离子浓度的变化,并利用非损伤微测技术测定植物根尖在不同处理下对Cd2+和Ca2+的动态吸收。结果表明,随着CdCl2处理浓度的增加,2种红树的根、胚轴、茎和叶4器官中的Cd2+含量均明显增加。而泌盐红树桐花树各器官中Cd2+含量均高于非泌盐红树秋茄,分别达到65%(根)、19%(胚轴)、203%(茎)和96%(叶)。利用非损伤微测技术测定Cd2+流,发现Cd2+内流能被Ca2+通道抑制剂LaCl3抑制,表明Cd2+主要通过Ca2+通道实现内流。在NaCl对Cd2+吸收的影响方面,低浓度NaCl(100~200 mmol/L)能促进秋茄对Cd2+的积累,但高浓度NaCl(400 mmol/L)抑制了桐花树和秋茄对Cd2+的吸收。这是由于:1)红树根系对Na+吸收增加,而Na+能与Cd2+竞争膜上转运蛋白上的金属离子结合位点从而减少Cd2+的吸收,2)NaCl促进了植物根尖对Ca2+的吸收,从而竞争性的抑制了Cd2+通过Ca2+通道的内流,最终减少了2种红树根系对Cd2+的吸收和积累。泌盐红树桐花树Cd2+含量高于非泌盐红树秋茄,表明桐花树根细胞质膜上的转运蛋白与Ca2+通道对Cd2+的吸收能力高于秋茄。
    Abstract: To explore the NaCl effects on Cd2+ uptake and transport in mangrove plants, Na+, Ca2+, Cd2+ accumulation in shoot and roots, and root Ca2+ and Cd2+ dynamic flux were examined in 3-month-old seedlings of the secretor mangrove Aegiceras corniculatum and non-secretor mangrove Kandelia obovate under NaCl and CdCl2 stress. The results show that Cd2+ content in roots, hypocotyl, stems, and leaves significantly increased with the increase of CdCl2 concentrations, in the two mangrove species. The Cd content in different organs of the secretor mangrove A. corniculatum was higher than in that of the non-secretor mangrove K. obovate, reaching 65% (root), 19% (hycopotyl), 203% (stem), and 96% (leaf) respectively. Using the non-invasive micro-test technique, the Cd2+ influx in roots upon CdCl2 stress was observed in two mangroves. Furthermore, the CdCl2-elicited Cd2+ influx was strongly inhibited by lanthanum chloride (LaCl3), a specific inhibitor of calcium channels. This indicates that Cd2+ enters root mainly through the calcium plasma membrane (PM) channels. We also examined the influence of NaCl on Cd2+ uptake. Low NaCl (100-200 mmol/L) was found to increase the Cd2+ uptake and accumulation, but a high NaCl (400 mmol/L) reduced the Cd2+ uptake in the two mangrove species. The possible explanations are: 1) Under NaCl treatment, the uptake of Na+ was increased by the root of mangrove. Thus, Na+ competed for metal-binding sites with Cd2+ in the PM transporters. As a result, the Cd2+ uptake was reduced in the two mangrove species. 2) NaCl increased Ca2+ influx under NaCl stress. This would inhibit the entry of Cd2+ through calcium channels, leading to a declined Cd2+ entry and accumulation in mangrove roots. Cd2+ accumulation in the secretor mangrove A. corniculatum was higher than the non-secretor mangrove K. obovate, indicating that the PM transporters and Ca2+ channels in A. corniculatum roots had a high capacity for Cd2+ uptake under NaCl and cadmium stress.
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  • 收稿日期:  2016-03-13
  • 发布日期:  2016-08-30

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