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松材线虫内生细菌与病原细菌的互作关系分析

曹锋 杨玲 龚姝榕 张林 牛秋红

曹锋, 杨玲, 龚姝榕, 张林, 牛秋红. 松材线虫内生细菌与病原细菌的互作关系分析[J]. 北京林业大学学报, 2016, 38(9): 25-33. doi: 10.13332/j.1000-1522.20160082
引用本文: 曹锋, 杨玲, 龚姝榕, 张林, 牛秋红. 松材线虫内生细菌与病原细菌的互作关系分析[J]. 北京林业大学学报, 2016, 38(9): 25-33. doi: 10.13332/j.1000-1522.20160082
CAO Feng, YANG Ling, GONG Shu-rong, ZHANG Lin, NIU Qiu-hong. Relationships between pathogenic bacterium and the endophytic bacteria isolated from Bursaphelenchus xylophilus.[J]. Journal of Beijing Forestry University, 2016, 38(9): 25-33. doi: 10.13332/j.1000-1522.20160082
Citation: CAO Feng, YANG Ling, GONG Shu-rong, ZHANG Lin, NIU Qiu-hong. Relationships between pathogenic bacterium and the endophytic bacteria isolated from Bursaphelenchus xylophilus.[J]. Journal of Beijing Forestry University, 2016, 38(9): 25-33. doi: 10.13332/j.1000-1522.20160082

松材线虫内生细菌与病原细菌的互作关系分析

doi: 10.13332/j.1000-1522.20160082
基金项目: 

国家自然科学基金项目(31570120、31100104)、河南省高等学校青年骨干教师资助项目(2012GGJS-154)、南阳师范学院研究生创新基金项目(2016CX006)。

详细信息
    作者简介:

    曹锋。主要研究方向:线虫的生物防治。Email:1220659082@qq.com地址:473061河南省南阳市卧龙路1638号南阳师范学院东区生物楼503。责任作者:牛秋红,博士,副教授。主要研究方向:微生物学与分子生物学。Email:qiuhongniu@hotmail.com地址:同上。

    曹锋。主要研究方向:线虫的生物防治。Email:1220659082@qq.com地址:473061河南省南阳市卧龙路1638号南阳师范学院东区生物楼503。责任作者:牛秋红,博士,副教授。主要研究方向:微生物学与分子生物学。Email:qiuhongniu@hotmail.com地址:同上。

Relationships between pathogenic bacterium and the endophytic bacteria isolated from Bursaphelenchus xylophilus.

  • 摘要: 采用16S rRNA基因文库和 illumina Miseq技术测序对松材线虫内生细菌群落的宏基因组进行初步分析,文库显示优势菌群为黄单胞菌科(22.41%)、鞘脂杆菌科(17.66%)、丛毛单胞菌科(14.71%)、根瘤菌科(10.17%)。采用稀释平板法,从松材线虫体内分离出5株内生细菌。利用形态学、生理学及16S rRNA 序列特征等对这些内生细菌进行了分类鉴定。研究发现这些内生细菌在体外均显示较强的杀线虫活性,其中克雷伯氏菌属sct5的杀虫活性最大,作用4 d松材线虫的死亡率即可达到100%。该细菌产生毒性蛋白酶并在体外快速形成生物膜。进一步研究发现,该内生细菌与杀线虫芽孢杆菌B16之间具有强亲和性,混合2个菌株sct5+B16作用1 d对松材线虫的防效为75%,明显好于单菌株sct5 (60%)和B16(40%)。而另一内生细菌肠杆菌属sct29与B16存在拮抗关系,两者不亲和,混合菌株sct29+B16作用1 d对松材线虫的防效仅为13%。本研究首次系统地报道了松材线虫可培养内生细菌的杀虫活性及其与杀线虫芽孢杆菌B16的亲和关系,对研制开发组合生防菌剂、提高生防菌株的稳定性等奠定理论基础,本研究从新的视角阐释了松材线虫内生细菌与病原细菌之间的互作关系。

     

  • [1] YANG B J, PAN H Y, TANG J, et al. Pine wilt disease [M]. Beijing: Chinese Forestry Press, 2003.
    [1] 杨宝君,潘宏阳,汤坚,等.松材线虫病[M].北京:中国林业出版社,2003.
    [2] 柴希民,蒋平.松材线虫病的发生与防治[M].北京:中国农业出版社,2003.
    [3] CHAI X M, JIANG P. Occurrence and prevention for pine wilt disease[M].Beijing: Chinese Agriculture Press, 2003.
    [4] 曾腓力,贲爱玲,郑敬荣,等. 美国松材线虫体表携带优势细菌的鉴定及致病性[J].浙江农林大学学报,2012, 29(5): 696-702.
    [5] ZENG F L, BEN A L, ZHENG J R, et al. Identification and pathogenicity of bacterial strains carried by American pine wood nematodes [J]. Journal of Zhejiang AF University, 2012, 29(5): 696-702.
    [6] 刘侃诚,曹东霞,田兴一,等. 松材线虫与拟松材线虫杂交及后代繁殖力[J].东北林业大学学报,2015, 43 (11):103-105.
    [7] LIU K C, CAO D X, TIAN X Y, et al. Hybridization between Bursaphelenchus xylophilus and B. mucronatus and reproduction of the offspring [J]. Journal of Northeast Forestry University, 2015, 43 (11):103-105.
    [8] 谈家金,冯志新.松材线虫病病理生理学研究进展[J]. 江西科学,2001, 19(1):27-30.
    [9] TAN J J, FENG Z X. Advance on physiopathology of pine wood nematode disease[J]. Jiangxi Science, 2001, 19(1):27-30.
    [10] 孙永春.南京中山陵发现松材线虫[J].江苏林业科技,1982, 12 (4): 47.
    [11] SUN Y C. Bursaphelenchus xylophilus was found in Nanjing Sun Yat-sens Mausoleum[J]. Jiangsu Forest Science and Technology, 1982, 12 (4): 47.
    [12] 朱克恭,朱正昌,严敖金. 松材线虫病的流行与研究进展[C]∥杨宝君. 中国松材线虫病的流行与治理.北京:中国林业出版社,1995: 297-314.
    [13] ZHU K G, ZHU Z C, YAN A J. Popular and research progress of Bursaphelenchus xylophilus[C]∥Popular and administration of pine wood nematode disease in China. Beijing: Chinese Forestry Press, 1995: 297-314.
    [14] NIU Q H, HUANG X W, ZHANG L, et al. A trojan horse mechanism of bacterial pathogenesis against nematodes [J]. Proceedings of the National Academy of Sciences, 2010, 107(38):16631-16636.
    [15] GUARNER F, MALAGELAD J R. Gut flora in health and disease[J]. The Lancet, 2003, 361: 512-19.
    [16] FORST S, DOWELS B,BOEMARE N, et al. Xenorhabdus and Photorhabdus spp. bugs that kill bugs[J]. Annual Reviews in Microbiology, 1997, 51(1) : 47-72.
    [17] NOUR S M, LAWRENCE J R, ZHU H,et al. Bacteria associated with cysts of the soybean cyst nematode (HeLerodera glycines) [J]. Applied and Environmental Microbiology, 2003, 69(1): 607-615.
    [18] HAEGEMAN A,VANHOLME B,JACOB J, et al. An endosymbiotic bacterium in a plant-parasitic nematode: member of a new Wolbachia supergroup[J]. International Journal for Parasitology, 2009, 39 (9):1045-1054.
    [19] 袁为敏,吴小芹,叶建仁,等. 松材线虫和拟松材线虫体内细菌的透射电镜观察及分离鉴定[J]. 微生物学报, 2011, 51(8): 1071-1077.
    [20] YUAN W M, WU X Q, YE J R, et al. Observation by transmission electron microscope and identification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus[J]. Acta Microbiologica Sinica, 2011, 51(8): 1071-1077.
    [21] ROSEN, S, SJOLLEMA K, VEEHUIS M, et al. A cytoplasmic lectin produced by the fungus Arthobotrys oligospora functions as a storage protein during saprophytic and parasitic growth[J]. Microbiology, 1997, 143(8): 2593-2604.
    [22] 杨维青,刘晓峰,黄震.铜绿假单胞菌临床菌株生物被膜的定量分析[J].中国抗生素杂志,2009,34(3):181-183.
    [23] YANG W Q, LIU X F, HUANG Z. Quantification for biofilm-forming of Pseudomonas aeruginosa isolate[J].Journal of Antibiotics in China, 2009,34(3):181-183.
    [24] 陈志谊,刘邮洲,刘永锋, 等.拮抗细菌菌株间的互作关系及其对生物防治效果的影响[J].植物病理学报,2005,35(6): 539-544.
    [25] CHEN Z Y, LIU Y Z, LIU Y F, et al. Compatibility between antagonistic bacterial strains and biocontrol efficacy by different bacterial combinations against Fusarium wilt of vegetables[J]. Acta Phytopathologica Sinica, 2005, 35(6):539-544.
    [26] XU P,LI W J,XU L H,et al. A microwave-based method for genomic DNA extraction from Actinomycetes[J]. Microbiology,2003,30 (4) : 82-84.
    [27] THOMPSON J,GIBSON T J,PLEWNIAK F,et al. The clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research,1997,25(24):4876-4882.
    [28] KUMAR S,TAMURA K,NEI M.MEGA 3: integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings in Bioinformatics, 2004, 5(2):150-163.
    [29] SCHLOSS P D, HANDELSMAN J. Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness[J]. Applied and Environmental Microbiology, 2005, 71(3):1501-6.
    [30] 潘沧桑. 松材线虫病研究进展(综述)[J]. 厦门大学学报(自然科学版),2011,50(2):476-483.
    [31] PAN C S. Development of studies on pinewood nematodes diseases[J]. Journal of Xiamen University (Natural Science), 2011,50(2):476-483.
    [32] 田雪亮,张祺玲,陈国华,等. 松材线虫伴生细菌多样性的宏基因组分析[J]. 微生物学报, 2010, 50(7):909-916.
    [33] TIAN X L, ZHANG Q L, CHEN G H, et al. Diversity of bacteria associated with pine wood nematode revealed by metagenome[J]. Acta Microbiologica Sinica, 2010, 50(7):909-916.
    [34] NIU Q H, ZHANG L, ZHANG K Q, et al. Changes in intestinal microflora of Caenorhabditis elegans following Bacillus nematocida B16 infection[J]. Scientific Reports, 2016, 6[2016-02-28]. DOI: 10.1038/srep20178.
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出版历程
  • 收稿日期:  2016-03-14
  • 刊出日期:  2016-09-30

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