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HPLC-MS/MS检测杜仲中绿原酸等4种活性成分的分析方法

李冉 齐芪 李赟 陈雪梅 盖颖

李冉, 齐芪, 李赟, 陈雪梅, 盖颖. HPLC-MS/MS检测杜仲中绿原酸等4种活性成分的分析方法[J]. 北京林业大学学报, 2016, 38(6): 123-129. doi: 10.13332/j.1000-1522.20160105
引用本文: 李冉, 齐芪, 李赟, 陈雪梅, 盖颖. HPLC-MS/MS检测杜仲中绿原酸等4种活性成分的分析方法[J]. 北京林业大学学报, 2016, 38(6): 123-129. doi: 10.13332/j.1000-1522.20160105
LI Ran, QI Qi, LI Yun, CHEN Xue-mei, GAI Ying. A method of HPLC-MS/MS to determine chlorogenic acid and other three kinds of active components in Eucommia ulmoids[J]. Journal of Beijing Forestry University, 2016, 38(6): 123-129. doi: 10.13332/j.1000-1522.20160105
Citation: LI Ran, QI Qi, LI Yun, CHEN Xue-mei, GAI Ying. A method of HPLC-MS/MS to determine chlorogenic acid and other three kinds of active components in Eucommia ulmoids[J]. Journal of Beijing Forestry University, 2016, 38(6): 123-129. doi: 10.13332/j.1000-1522.20160105

HPLC-MS/MS检测杜仲中绿原酸等4种活性成分的分析方法

doi: 10.13332/j.1000-1522.20160105
基金项目: 

北京高等学校“

青年英才计划”

项目(YETP0755)、北京市自然科学基金项目(5141001)

详细信息
    作者简介:

    李冉。主要研究方向:植物分子生物学。Email:li_ran329@sina.com 地址:100083 北京市海淀区清华东路35号 北京林业大学生物科学与技术学院。

    责任作者:

    盖颖,副教授。主要研究方向:生物化学与分子生物学。Email:gaiying@bjfu.edu.cn 地址:同上。

A method of HPLC-MS/MS to determine chlorogenic acid and other three kinds of active components in Eucommia ulmoids

  • 摘要: 杜仲作为一种传统的中药材,其活性成分主要包括绿原酸、桃叶珊瑚苷、京尼平苷和京尼平苷酸等。本研究建立了一种HPLC-MS/MS(高效液相色谱质谱联用仪)检测的方法,可同时检测杜仲中桃叶珊瑚苷、京尼平苷、京尼平苷酸和绿原酸的含量。建立的色谱条件:色谱柱XD-C18,流动相为0.1%甲酸水溶液和甲醇,流速0.15mL/min,柱温30℃。质谱条件: ESI离子源作为裂解源,裂解温度280℃,源内电压4kV,鞘气辅助气为氮气,流速30psi,雾化气为氦气,流速10psi。结果表明:4种目标化合物的色谱峰分离情况良好,质谱鉴定为目标物;绿原酸、桃叶珊瑚苷、京尼平苷和京尼平苷酸标准曲线的相关性系数分别为0.9935、0.9942、0.9969、0.9948;最低检测限的范围是7.603~8.853pmol;加样回收率范围是91.838%~108.326%。测定得到杜仲叶中桃叶珊瑚苷的含量为(20.552±4.032)ng/mg干质量,京尼平苷酸含量为(6.913±0.654)ng/mg干质量,绿原酸含量为(25.986±3.412)ng/mg干质量,京尼平苷含量为(0.205±0.015 )ng/mg干质量。利用该方法在7种植物组织中进行方法验证,测定得到4种目标化合物的含量,表明本方法有效且适用性广。

     

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出版历程
  • 收稿日期:  2016-03-28
  • 修回日期:  2016-03-28
  • 刊出日期:  2016-06-30

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