N,O-羧甲基壳聚糖抗菌染料的制备研究
Preparation of an antibacterial N,O-carboxymethyl chitosan dye.
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摘要: 以壳聚糖(CTS)和活性艳蓝KN-R染料为原材料,通过两步反应法制备新型N,O-羧甲基壳聚糖染料,用红外光谱(FTIR)、X射线衍射(XRD)、元素分析仪分析其结构和化学基团,并用紫外可见分光光度计对其水溶性进行检测;采用常压浸渍法制备N,O-羧甲基壳聚糖染料染色薄木,用光谱光度仪进行表面颜色及日晒和水洗色差测量;用菌落计数法定量表征其抗菌性能。结果表明:CTS分子中大量—OH、少量—NH2基团被—CH2COOH基团取代,染料中的—SO-3和N,O-羧甲基壳聚糖中质子化的—NH+3发生反应;羧甲基化和染料接枝反应使得CTS结晶度下降;N,O-羧甲基壳聚糖染料的水溶性得到显著改善;N,O-羧甲基壳聚糖染料可明显改善薄木的染色效果,且染色薄木的耐日晒和耐水洗牢度均提高;N,O-羧甲基壳聚糖染料染色薄木的抗菌性能优于活性艳蓝KN-R染色薄木。Abstract: Chitosan and reactive brilliant blue KN-R were used to prepare a novel N,O-carboxymethyl chitosan dye by a two-step reaction. FTIR, XRD and elemental analyzer were employed to explore the chemical groups and structure of the obtained new dye. Besides, the water solubility was tested using a UV-visible spectrophotometer. The color parameters and color fastness of N,O-carboxymethyl chitosan dye dyed veneers with impregnating in atmospheric pressure were measured using a color measuring instrument. The antibacterial property of N,O-carboxymethyl chitosan dye treated veneers was specific evaluated by colony counting method. The results showed that: numerous —OH and little —NH2 were replaced by —CH2COOH, and —SO-3 of the dye had a chemical reaction with protonated —NH+3 of N,O-carboxymethyl chitosan. The crystallinity of CTS was obviously decreased while the water solubility of N,O-carboxymethyl chitosan dye was notably improved. N,O-carboxymethyl chitosan dye obviously improved the dyeing effects and light fastness as well as water fastness were both enhanced. Additionally, the antibacterial properties of N,O-carboxymethyl chitosan dye were superior to reactive brilliant blue KN-R.
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