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    刘鹏, 孙丽丽, 张琪慧, 曹传旺. 舞毒蛾小分子热激蛋白基因分析及对甲萘威胁迫的响应[J]. 北京林业大学学报, 2017, 39(1): 78-84. DOI: 10.13332/j.1000-1522.20160179
    引用本文: 刘鹏, 孙丽丽, 张琪慧, 曹传旺. 舞毒蛾小分子热激蛋白基因分析及对甲萘威胁迫的响应[J]. 北京林业大学学报, 2017, 39(1): 78-84. DOI: 10.13332/j.1000-1522.20160179
    LIU Peng, SUN Li-li, ZHANG Qi-hui, CAO Chuan-wang. Analysis of smHsp genes in Lymantria dispar and its response to carbaryl stress[J]. Journal of Beijing Forestry University, 2017, 39(1): 78-84. DOI: 10.13332/j.1000-1522.20160179
    Citation: LIU Peng, SUN Li-li, ZHANG Qi-hui, CAO Chuan-wang. Analysis of smHsp genes in Lymantria dispar and its response to carbaryl stress[J]. Journal of Beijing Forestry University, 2017, 39(1): 78-84. DOI: 10.13332/j.1000-1522.20160179

    舞毒蛾小分子热激蛋白基因分析及对甲萘威胁迫的响应

    Analysis of smHsp genes in Lymantria dispar and its response to carbaryl stress

    • 摘要: 昆虫小分子热激蛋白(smHsp)是分子质量在12~43 kDa、具有分子伴侣活性的应激蛋白,可以在环境胁迫下帮助蛋白质折叠和转运。本文为明确舞毒蛾的smHsp基因特性及对杀虫剂甲萘威胁迫的响应,从舞毒蛾无参照全转录本文库中鉴定获得6个小分子热激蛋白家族基因,全长基因开放阅读框为444~567 bp,编码147~188个氨基酸,理论等电点为5.58~6.17。根据分子质量大小分别命名为LdHsp17.0、LdHsp18.7、LdHsp19.1、LdHsp20.3、LdHsp21.3和LdHsp21.4。亚致死剂量(LC5、LC10和LC30)的甲萘威处理可诱导舞毒蛾smHsp家族中LdHsp20.3、LdHsp19.1和LdHsp17.0上调表达,但抑制了LdHsp21.4、LdHsp21.3和LdHsp18.7的表达,且甲萘威处理对smHsp基因表达量的影响存在质量浓度和剂量效应。以上结果为进一步研究舞毒蛾小分子热激蛋白功能及其参与对杀虫剂抗性的机制提供了基础。

       

      Abstract: Small heat shock proteins of insect with molecular weight from 12 to 43 kDa are stress proteins with molecular chaperone activity and can assist protein folding and transporting under environmental stress. In present study, the full length of six smHsp cDNA was identified from Lymantria dispar transcriptome. The open reading frames (ORF) of smHsps were from 444 to 567 bp encoding proteins of 147-188 amino acid residues with the theoretical pI of 5.58-6.17. These smHsps were named as LdHsp17.0, LdHsp18.7, LdHsp19.1, LdHsp20.3, LdHsp21.3 and LdHsp21.4 according to their molecular mass. The effects of sublethal concentrations (LC5, LC10 and LC30) of carbaryl on smHsp gene expression in L. dispar larvae were analyzed using real-time RT-PCR technology. The results showed that the expressions of LdHsp20.3, LdHsp19.1 and LdHsp17.0 were induced while those of LdHsp21.4, LdHsp21.3 and LdHsp18.7 were inhibited under three sublethal concentrations of carbaryl. The smHsp gene expressions were in concentration- and time-dependent way. Our study provides the basis for further studying the functions of smHsp and their roles in insecticide resistance in L. dispar.

       

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