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2个芍药品种不同花器官香气成分分析

宋朝伟 于晓南

宋朝伟, 于晓南. 2个芍药品种不同花器官香气成分分析[J]. 北京林业大学学报, 2017, 39(2): 92-99. doi: 10.13332/j.1000-1522.20160198
引用本文: 宋朝伟, 于晓南. 2个芍药品种不同花器官香气成分分析[J]. 北京林业大学学报, 2017, 39(2): 92-99. doi: 10.13332/j.1000-1522.20160198
SONG Chao-wei, YU Xiao-nan. Analysis of the aroma composition of different flower organs of two peony varieties[J]. Journal of Beijing Forestry University, 2017, 39(2): 92-99. doi: 10.13332/j.1000-1522.20160198
Citation: SONG Chao-wei, YU Xiao-nan. Analysis of the aroma composition of different flower organs of two peony varieties[J]. Journal of Beijing Forestry University, 2017, 39(2): 92-99. doi: 10.13332/j.1000-1522.20160198

2个芍药品种不同花器官香气成分分析

doi: 10.13332/j.1000-1522.20160198
基金项目: 

安徽省教育厅高校自然科学研究重点项目 KJ2015A433

国家自然科学基金项目 31400591

详细信息
    作者简介:

    宋朝伟,副教授,北京林业大学访问学者。主要研究方向:观赏园艺与园林植物。Email:1245177088@qq.com  地址:236031 安徽省阜阳市阜南路465号阜阳职业技术学院

    责任作者:

    于晓南,教授,博士生导师。主要研究方向:观赏园艺与园林植物。Email:yuxiaonan626@126.com  地址:100083 北京市海淀区清华东路35号北京林业大学园林学院

  • 中图分类号: S682.1+2

Analysis of the aroma composition of different flower organs of two peony varieties

  • 摘要: ‘巧玲’和‘杨妃出浴’是杂交选育芳香型芍药新品种的重要亲本材料。为了弄清其不同花器官香气成分、含量及其分布规律,进而为芍药花香相关功能基因的分离、鉴定、克隆、时空表达及芳香型芍药分子育种等后续研究提供一定的理论依据。本文以典型芳香型芍药品种‘巧玲’与‘杨妃出浴’为试材,以淡香型芍药品种‘粉玉奴’为对照,采用顶空固相微萃取和气相-质谱联用技术,检测分析其花瓣、瓣化雄蕊、雌蕊、萼片4个不同花器官的香气成分。结果表明:‘杨妃出浴’含有29种香气成分,主要为香叶醇(27.62%)、苯乙醇(18.00%)、3, 7-二甲基-2-辛烯醇(14.56%)、α-蒎烯(9.79%),鉴于‘粉玉奴’花瓣样品中未能检测到以上香气成分,因此可初步确定香叶醇、苯乙醇、3, 7-二甲基-2-辛烯醇、α-蒎烯等为‘杨妃出浴’花冠释放香气的关键成分。‘巧玲’含有41种香气成分,主要为芳樟醇(30.06%)、桉油精(23.28%)、苯乙醇(22.09%)、乙酸叶醇酯(11.86%)、石竹烯(5.24%),其中芳樟醇与桉油精含量特别高,对照分析可初步确定‘巧玲’花香主要来源于芳樟醇、苯乙醇、桉油精与石竹烯的贡献。同时,2个品种不同花器官之间香气物质的释放量均表现出明显差异,其中瓣化雄蕊中均为最高,外层花瓣次之,而雌蕊与萼片中较少,与感官上香气的初步判断相吻合。因此,‘巧玲’和‘杨妃出浴’花香成分在不同花器官部位的空间表达上具有明显差异性。

     

  • 图  1  ‘巧玲’与‘杨妃出浴’盛花期不同花器官的形态

    Figure  1.  Morphology of different floral organs of 'Qiao Ling' and 'Yang Fei Chuyu' during full-flowering stage

    图  2  ‘巧玲’盛花期花瓣总离子流图

    7.顺式-3-己烯醇;8.1-己醇;9.甲基庚烯酮;10.月桂烯;11.右旋甲基庚烯醇;12.乙酸叶醇酯;13.乙酸己酯;14.1, 5-二甲基-1, 5-环辛二烯;15.桉油精;16.芳樟醇;18.苯乙醇;19.1, 4-二甲氧基苯;20.3-甲基-3-癸烯-2-酮;21.α-松油醇;22.(R)-香茅醇;23.乙酸苯乙酯;24.乙酸香茅酯;26.石竹烯。

    Figure  2.  Total ionic chromatogram of petal of 'Qiao Ling' during full-flowering stage

    7, (Z)-3-Hexen-1-ol; 8, 1-Hexanol; 9, 6-methyl-5-Hepten-2-one; 10, Myrcene; 11, dl-6-Methyl-5-hepten-2-ol; 12, Leaf acetate; 13, Acetic acid, hexyl ester; 14, 1, 5-dimethyl-1, 5-Cyclooctadiene; 15, Eucalyptol; 16, Linalool; 18, Phenylethyl alcohol; 19, 1, 4-dimethoxy-Benzene; 20, 3-methyl-3-Decen-2-one; 21, α-terpineol; 22, (R)-citronellol; 23, Acetic acid, 2-phenylethyl ester; 24, Citronellyl acetate; 26, Caryophyllene.

    图  3  ‘杨妃出浴’盛花期花瓣总离子流图

    3.顺式-3-己烯醇;4.1-己醇;5.α-蒎烯;6.月桂烯;7.乙酸叶醇酯;8.乙酸己酯;9.右旋柠檬油精;10.3-(4-甲基-3-戊烯基)-呋喃;11.芳樟醇;12.马鞭草烯酮;13.橙花醇;14.3, 7-二甲基-2-辛烯醇;15.香叶醇;16.反式-柠檬醛;17.乙酸香叶酯;18.石竹烯;20.橙花叔醇。

    Figure  3.  Total ionic chromatogram of petal of 'Yang Fei Chuyu' during full-flowering stage

    3, (Z)-3-Hexen-1-ol; 4, 1-Hexanol; 5, alpha.-Pinene; 6, Myrcene; 7, Leaf acetate; 8, Acetic acid, hexyl ester; 9, D-Limonene; 10, 3-(4-methyl-3-pentenyl)-Furan; 11, Linalool; 12, (1S)-4, 6, 6-trimethyl-Bicyclo[3.1.1]hept-3-en-2-one; 13, Orange blossom alcohol; 14, 3, 7-dimethyl-2-Octen-1-ol; 15, (E)-3, 7-dimethyl-2, 6-Octadien-1-ol; 16, (E)-3, 7-dimethyl-2, 6-Octadienal; 17, Geranyl acetate; 18, Caryophyllene; 20, Nerolidol.

    表  1  不同花器官香气成分及释放量

    Table  1.   Aromatic compounds and release amounts of different floral organs

    μg

    香气成分
    Aromatic compounds
    ‘巧玲’‘Qiao Ling’ ‘杨妃出浴’‘Yang Fei Chuyu’ CK
    花瓣
    Petal
    瓣化雄蕊
    Petalody
    stamen
    雌蕊
    Pistil
    萼片
    Sepal
    花瓣
    Petal
    瓣化雄蕊
    Petalody
    stamen
    雌蕊
    Pistil
    萼片
    Sepal
    花瓣
    Petal
    醇类Alcohols 顺式-3-己烯醇(Z)-3-Hexen-1-ol 6.08±0.17 1.42±0.04 0.00 20.08±0.24 4.56±0.09 1.62±0.03 0.00 6.90±0.15 4.18±0.09
    反式-2-己烯醇(E)-2-Hexen-1-ol 0.00 0.00 0.00 2.42±0.07 0.00 0.00 0.00 0.00 0.00
    1-己醇1-Hexanol 0.38±0.03 0.31±0.03 0.00 1.74±0.05 1.62±0.08 0.00 0.00 1.68±0.03 0.86±0.02
    右旋甲基庚烯醇
    dl-6-Methyl-5-hepten-2-ol
    0.24±0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    芳樟醇Linalool 60.12±0.61 45.04±0.26 3.25±0.09 0.00 8.80±0.12 12.76±0.18 10.60±0.31 0.00 74.48±0.46
    苯乙醇Phenylethyl alcohol 27.46±0.22 44.18±0.31 5.82±0.11 0.00 0.00 36.00±0.37 38.80±0.62 0.00 0.00
    松油醇p-menth-1-en-8-ol 0.00 0.31±0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    α-松油醇α-terpineol 0.44±0.02 5.36±0.13 0.64±0.05 0.00 0.00 0.00 0.00 0.00 0.00
    (R)-香茅醇(R) - citronellol 0.88±0.02 0.35±0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    橙花醇Orange blossom alcohol 0.00 0.22±0.01 0.00 0.00 6.30±0.19 4.66±0.11 0.96±0.04 0.00 0.00
    香叶醇(E)-3, 7-dimethyl-2, 6-Octadien-1-ol 0.00 0.00 0.00 0.00 87.82±0.65 55.24±0.49 18.64±0.25 0.00 0.00
    3, 7-二甲基-2-辛烯醇
    3, 7-dimethyl-2-Octen-1-ol
    0.00 0.00 0.00 0.00 17.94±0.14 29.12±0.33 3.14±0.06 0.00 0.00
    双环[3.1.0]己烷-2-醇Bicyclo[3.1.0]hexan-2-ol 0.00 0.35±0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    4-萜烯醇terpinen-4-ol 0.00 0.16±0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.42±0.01
    橙花叔醇nerolidol 0.00 0.00 0.75±0.03 0.00 1.18±0.08 1.96±0.06 0.00 0.00 0.00
    L-薄荷醇L-menthol 0.00 0.00 0.00 1.36±0.04 0.00 0.00 0.00 0.00 0.00
    酯类Esters 乙酸叶醇酯Leaf acetate 29.16±0.18 6.90±0.18 29.25±0.24 48.46±0.52 3.34±0.07 3.54±0.11 5.54±0.18 46.03±0.67 25.76±0.37
    (E)-2-己烯醇乙酸酯
    (E)-2-Hexen-1-ol, acetate
    0.00 0.00 0.00 8.18±0.31 0.00 0.00 0.00 5.40±0.11 0.00
    乙酸香茅酯Citronellyl acetate 0.74±0.06 0.48±0.03 0.00 0.00 0.00 0.68±0.03 0.00 0.00 0.00
    乙酸香叶酯Geranyl acetate 0.00 0.00 0.00 0.00 5.60±0.11 7.16±0.12 2.82±0.09 0.00 0.00
    乙酸己酯Acetic acid,hexyl ester 6.76±0.09 1.04±0.05 4.90±0.09 10.98±0.17 0.80±0.05 1.04±0.05 3.16±0.11 8.56±0.22 3.88±0.14
    乙酸辛酯Acetic acid, octyl ester 0.00 0.00 0.28±0.02 0.00 0.00 0.00 0.00 0.00 0.00
    乙酸苯乙酯Acetic,2-phenylethyl ester 5.05±0.07 19.42±0.27 49.42±0.22 0.00 0.00 0.00 5.88±0.32 0.00 0.00
    3-甲基丁酸苯乙酯
    3-methyl butyrate
    0.00 0.00 0.00 0.00 0.00 0.00 1.46±0.07 0.00 0.00
    2-甲基丁基戊酸酯
    2-methyl butyl ester
    0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.02±0.03 0.00
    3-甲基丁基丁酸酯
    3-methyl butyl butyrate
    0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.64±0.08 0.00
    (Z)-3-己烯基正戊酸酯(Z)-3-hexenyl positive acid ester 0.00 0.00 0.64±0.06 1.02±0.08 0.00 0.00 0.00 3.80±0.18 0.34±0.01
    顺式-3-己烯醇苯甲酸酯(Z)-3-Hexen-1-ol-benzoate 0.00 0.00 0.00 1.56±0.06 0.00 0.00 0.00 8.68±0.13 0.00
    萜烯类Terpenes 月桂烯Myrcene 0.38±0.01 1.26±0.09 0.00 0.00 6.24±0.14 3.96±0.08 0.00 0.00 0.65±0.02
    1, 5 -二甲基-1, 5-环辛二烯
    1, 5-dimethyl-1, 5-Cyclooctadiene
    0.24±0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.47±0.06
    桉油精Eucalyptol 38.62±0.18 46.56±0.46 6.28±0.12 0.00 0.00 0.00 0.00 0.00 3.59±0.08
    石竹烯Aryophyllene 3.20±0.11 6.48±0.21 13.96±0.11 4.06±0.13 1.74±0.06 3.24±0.05 9.30±0.16 0.00 0.00
    α-石竹烯α-Caryophyllene 0.00 0.36±0.05 0.74±0.05 0.00 0.00 0.00 0.00 0.00 0.00
    水合桧烯Sabinene hydrate 0.00 0.42±0.03 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    α-蒎烯α-Pinene 0.00 0.22±0.01 0.00 0.00 26.38±0.18 19.58±0.21 0.00 0.00 0.00
    β-水芹烯β-Phellandrene 0.00 6.60±0.19 0.36±0.02 0.00 0.00 0.00 0.00 0.00 0.00
    古柏烯Copaene 0.00 0.00 4.58±0.13 3.26±0.08 0.00 0.00 0.00 8.18±0.17 0.00
    α-萜品烯α-terpinen 0.00 0.36±0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    1, 4-环己二烯1, 4-Cyclohexadiene 0.00 0.58±0.07 0.26±0.01 0.00 0.00 0.00 0.00 0.00 0.00
    右旋柠檬油精D-Limonene 0.00 0.46±0.03 0.00 0.00 2.70±0.09 1.54±0.07 0.00 0.00 0.00
    α-红没药烯α-Bisabolene 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.76±0.05
    α-荜澄茄油α-Cubebene 0.00 0.00 3.30±0.08 0.00 0.00 0.00 3.05±0.13 0.00 0.00
    反式-β-罗勒烯(E)-β-ocimene 0.00 0.20±0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    顺式-β-罗勒烯(Z)-β-ocimene 0.00 6.14±0.19 0.00 0.00 0.00 0.00 0.00 0.00 26.81±0.17
    烷烃类Alkanes 1, 4-二甲氧基苯
    1, 4-dimethoxy-Benzene
    0.24±0.03 0.00 0.00 0.00 0.00 1.96±0.08 4.66±0.23 0.00 9.24±0.11
    3-(4-甲基-3-戊烯基)-呋喃3-(4-methyl-3-pentenyl)-Furan 0.00 0.00 0.00 0.00 4.56±0.16 3.30±0.06 0.00 0.00 0.00
    醛类Aldehydes 反式-柠檬醛(E)-lemon aldehyde 0.00 0.00 0.00 0.00 1.94±0.05 1.16±0.05 0.82±0.04 0.00 0.00
    酮类Ketones 甲基庚烯酮6-methyl-5-Hepten-2-one 0.38±0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    3-甲基-3-癸烯-2-酮3-methyl-3-Decen-2-one 0.34±0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
    苯并呋喃酮Benzofuranone 0.00 0.94±0.04 1.44±0.06 0.00 0.00 0.00 0.00 0.00 0.00
    马鞭草烯酮Verbenone 0.00 0.00 0.00 0.00 1.76±0.06 6.98±0.18 0.00 0.00 0.00
    合计Total 180.65 192.01 95.87 103.12 183.28 195.50 108.83 97.89 152.44
    下载: 导出CSV

    表  2  不同花器官香气成分的相对含量与成分种类数

    Table  2.   Aromatic compounds' relative contents and component number of different floral organs

    %
    化合物Compounds ‘巧玲’‘Qiao Ling’ ‘杨妃出浴’‘Yang Fei Chuyu’ CK
    花瓣Petal 瓣化雄蕊Petalody stamen 雌蕊Pistil 萼片Sepal 花瓣Petal 瓣化雄蕊Petalody stamen 雌蕊Pistil 萼片Sepal 花瓣Petal
    醇类Alcohols 47.80(7) 48.84(10) 15.23(4) 22.80(4) 64.21(7) 70.68(7) 26.07(5) 4.29(2) 39.97(4)
    酯类Esters 21.33(4) 11.86(4) 32.22(5) 55.10(5) 4.87(3) 6.21(4) 39.43(5) 68.56(7) 14.98(3)
    萜烯类Terpenes 20.45(4) 34.82(12) 14.74(7) 3.66(2) 18.53(4) 14.16(4) 6.15(2) 4.09(1) 16.60(5)
    烷烃类Alkanes 0.12(1) 0.00(0) 0.00(0) 0.00(0) 2.28(1) 2.63(2) 6.33(1) 0.00(0) 4.60(1)
    醛类Aldehydes 0.00(0) 0.00(0) 0.00(0) 0.00(0) 0.97(1) 0.58(1) 0.41(1) 0.00(0) 0.00(0)
    酮类Ketones 0.36(2) 0.47(1) 0.72(1) 0.00(0) 0.88(1) 3.49(1) 0.00(0) 0.00(0) 0.00(0)
    合计Total 90.06(18) 95.99(27) 62.91(17) 81.56(11) 91.74(17) 97.75(19) 78.39(14) 76.94(10) 76.15(13)
    注:括号内数字为成分种类数。Note: number in the bracket means component types.
    下载: 导出CSV
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  • 收稿日期:  2016-06-16
  • 修回日期:  2016-10-20
  • 刊出日期:  2017-02-01

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