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橡子壳色素抗氧化活性研究

张亚亭 李德海 包怡红

张亚亭, 李德海, 包怡红. 橡子壳色素抗氧化活性研究[J]. 北京林业大学学报, 2017, 39(4): 94-100. doi: 10.13332/j.1000-1522.20160281
引用本文: 张亚亭, 李德海, 包怡红. 橡子壳色素抗氧化活性研究[J]. 北京林业大学学报, 2017, 39(4): 94-100. doi: 10.13332/j.1000-1522.20160281
ZHANG Ya-ting, LI De-hai, BAO Yi-hong. Antioxidant activity on the pigments from acorn shell[J]. Journal of Beijing Forestry University, 2017, 39(4): 94-100. doi: 10.13332/j.1000-1522.20160281
Citation: ZHANG Ya-ting, LI De-hai, BAO Yi-hong. Antioxidant activity on the pigments from acorn shell[J]. Journal of Beijing Forestry University, 2017, 39(4): 94-100. doi: 10.13332/j.1000-1522.20160281

橡子壳色素抗氧化活性研究

doi: 10.13332/j.1000-1522.20160281
基金项目: 

中央高校基本科研业务费专项 2572014CA19

中央高校基本科研业务费专项 2572014EA02

详细信息
    作者简介:

    张亚亭。主要研究方向:食品化学及植物有效成分。Email: coldfront_cool@126.com  地址: 150040  黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院

    责任作者:

    李德海,博士,副教授。主要研究方向:食品化学及植物有效成分。Email: lidehaineau@163.com  地址:同上

  • 中图分类号: S789.2

Antioxidant activity on the pigments from acorn shell

  • 摘要: 本研究以常见的抗氧化剂Vc为对照,对橡子壳色素主要成分和体内外抗氧化活性进行了研究分析。结果表明,橡子壳色素中含丰富的多酚类和黄酮类物质,其中总多酚含量达到27.8%,总黄酮含量8.6%。体外抗氧化实验结果表明,橡子壳色素对DPPH·的清除能力和对Fe3+的还原能力均与其质量浓度呈正相关,其中对DPPH·清除率的半数有效剂量(IC50=10 μg/mL)大于Vc组(IC50=5 μg/mL),当橡子壳色素质量浓度在40 μg/mL时达到了Vc的清除能力;当橡子壳色素质量浓度为1 mg/mL时,橡子壳色素还原能力(A710 nm为0.98)接近Vc的还原能力(A710 nm为1.17)。体内抗氧化实验采用对除空白组外的各组实施60Coγ照射进行氧化损伤实验,分析各组的抗氧化能力。结果表明,模型组小鼠体质量和各器官质量与空白组相比显著下降(P<0.05),并且辐照的各组小鼠都出现了食欲不振、精神萎靡等不良现象,表明氧化应激模型建立成功;橡子壳色素组的小鼠器官质量显著高于模型组(P<0.05),低于空白组但无显著差异(P>0.05),该结果表明橡子壳色素对经60Coγ射线照射的小鼠器官有一定的修复作用。实验小鼠血清中的抗氧化性相关酶以及氧化产物的测定结果进一步表明,橡子壳色素低、中、高剂量组均能显著提高小鼠血清中SOD和CAT的活性,并且显著降低MAD含量(P<0.05),其中100 mg/(kg·d)剂量组橡子壳色素的作用效果最好,与同质量浓度Vc作用效果相当。橡子壳色素具有较好的体内和体外抗氧化活性,并且体内100 mg/(kg·d)剂量的橡子壳色素与同剂量下Vc的抗氧化效果相当,作为天然抗氧剂具有较好的应用前景。

     

  • 图  1  样品质量浓度对DPPH·清除率的影响

    Figure  1.  Influence of sample concentration on the clearance rate of DPPH·

    图  2  样品质量浓度对还原力的影响

    Figure  2.  Influence of sample concentration on reducing power

    图  3  橡子壳色素对小鼠血清SOD活性的影响

    1.模型组;2.空白组; 3.维生素C组; 4.色素低剂量组; 5.色素中剂量组; 6.色素高剂量组; 不同小写字母表示各组之间有显著差异(P<0.05)。下图同此。

    Figure  3.  Influence of acorn shell pigment on serum SOD activity of mice

    1, model group; 2, blank group; 3, Vc group; 4, low level group of acorn shell pigments; 5, middle level group of acorn shell pigments; 6, high level group of acorn shell pigments; different small letters within a group mean significant difference at P < 0.05 level. The same below.

    图  4  橡子壳色素对小鼠血清过氧化氢酶(CAT)活性的影响

    Figure  4.  Effects of acorn shell pigment on serum CAT activity of mice

    图  5  橡子壳色素对小鼠血清MDA含量的影响

    Figure  5.  Effects of acorn shell pigment on serum MDA levels in mice

    表  1  实验期间各组小鼠体重变化

    Table  1.   Mass changes of mice during the experiment

    组别
    Group
    剂量Dose/
    (mg·kg-1·d-1)
    第1天
    1st day/g
    第7天
    7th day/g
    第14天
    14th day/g
    第21天
    21st day/g
    第28天
    28th day/g
    辐照后7 d
    7th day after irradiation/g
    模型组Model group18.70±0.39aA22.72±0.75aB24.93±1.03aC28.19±1.39aD30.85±1.78aD25.68±1.73aC
    空白组Blank group18.44±0.51aA23.04±0.94aB25.02±1.33aC28.97±1.4aD30.97±1.27aD30.22±2.80cD
    Vc组Vc group10018.28±0.55aA22.50±1.14aB25.42±1.54aC28.70±1.27aD30.73±1.87aD28.04±1.63bD
    橡子壳色素组
    Acorn shell pigment group
    5018.63±0.78aA22.59±0.53aB25.77±1.25aC28.69±1.08aD30.24±1.65aD28.23±1.34bD
    10018.98±0.99aA22.91±0.86aB24.28±1.77aC28.31±2.00aD29.88±1.90aD27.93±1.71bD
    20018.88±0.48aA22.91±0.69aB24.66±1.58aC28.05±1.52aD29.01±2.11aD28.45±1.98bD
    注:不同小写字母表示每一组各列之间差异显著(P<0.05);不同大写字母表示各组每一行之间差异显著(P<0.05)。下同。Notes: different small letters within a column mean significant difference at P<0.05 level; different capital letters within a row mean significant difference at P<0.05 level. The same below.
    下载: 导出CSV

    表  2  各实验组的小鼠脏器系数

    Table  2.   Mouse organ coefficient of each experiment group

    组别
    Group
    剂量Dose/
    (mg·kg-1·d-1)
    心脏指数
    Heart index/%
    肝脏指数
    Hepatic index/%
    脾脏系数
    Spleen index/%
    肾指数
    Kidney index/%
    模型组Model group0.48±0.008 8a4.06±0.10a0.20±0.007 5a1.45±0.098a
    空白组Blank group0.67±0.005 9b5.60±0.066b0.25±0.009 4b1.76±0.087b
    Vc组Vc group1000.62±0.007 9b5.44±0.095b0.24±0.008 6b1.69±0.097b
    橡子壳色素组
    Acorn shell pigment group
    500.60±0.0090b5.04±0.083b0.25±0.0087b1.74±0.078b
    1000.63±0.0054b4.98±0.077b0.24±0.0082b1.73±0.092b
    2000.64±0.0074b5.27±0.063b0.24±0.0076b1.74±0.088b
    注:不同小写字母表示每一组各列之间有显著差异(P<0.05)。Note: different small letters within a column mean significant difference at P<0.05 level.
    下载: 导出CSV
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  • 收稿日期:  2016-09-28
  • 修回日期:  2016-11-21
  • 刊出日期:  2017-04-01

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