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用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响

姜礅 孟昭军 严善春

姜礅, 孟昭军, 严善春. 用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响[J]. 北京林业大学学报, 2017, 39(2): 58-63. doi: 10.13332/j.1000-1522.20160291
引用本文: 姜礅, 孟昭军, 严善春. 用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响[J]. 北京林业大学学报, 2017, 39(2): 58-63. doi: 10.13332/j.1000-1522.20160291
JIANG Dun, MENG Zhao-jun, YAN Shan-chun. Effects of partially spraying Larix olgensis seedlings with exogenous methyl jasmonate on the defensive enzyme activities of Dendrolimus superans larvae[J]. Journal of Beijing Forestry University, 2017, 39(2): 58-63. doi: 10.13332/j.1000-1522.20160291
Citation: JIANG Dun, MENG Zhao-jun, YAN Shan-chun. Effects of partially spraying Larix olgensis seedlings with exogenous methyl jasmonate on the defensive enzyme activities of Dendrolimus superans larvae[J]. Journal of Beijing Forestry University, 2017, 39(2): 58-63. doi: 10.13332/j.1000-1522.20160291

用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响

doi: 10.13332/j.1000-1522.20160291
基金项目: 

国家公益性行业科研专项 200904021

详细信息
    作者简介:

    姜礅,博士生。主要研究方向:昆虫化学生态。Email: 821880041@qq.com   地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学林学院

    责任作者:

    严善春,教授,博士生导师。主要研究方向:昆虫化学生态。Email: yanshanchun@126.com   地址:同上

  • 中图分类号: S791.22;S763.42

Effects of partially spraying Larix olgensis seedlings with exogenous methyl jasmonate on the defensive enzyme activities of Dendrolimus superans larvae

  • 摘要: 为明确茉莉酸甲酯局部处理林木对落叶松毛虫解毒代谢的影响,本研究以3种不同浓度的茉莉酸甲酯(MeJA,0.01、0.10和1.00mmol/L)局部处理长白落叶松苗的侧枝,分析不同处理对取食其针叶的落叶松毛虫3~5龄幼虫体内保护酶和解毒酶活性的影响。研究发现,3个浓度的MeJA局部处理长白落叶松对松毛虫幼虫体内保护酶和解毒酶活性均有显著影响(P < 0.05),酶活变化趋势一致,但未呈浓度依赖关系。各处理组幼虫体内的SOD和POD活性均在第4龄显著高于CK(P < 0.05),但3、5龄幼虫体内的SOD和POD活性与CK差异不显著(P>0.05);而ACP、AKP和GSTs活性先在第3龄低于CK、第4龄均显著低于CK(P < 0.05),后在第5龄不同程度地高于CK。表明用MeJA局部喷施能诱导提高长白落叶松的系统抗性,且其对食叶害虫具有广谱性,能干扰落叶松毛虫的解毒代谢,抵御其危害;落叶松毛虫能在逐渐适应后,通过提高保护酶和解毒酶的活性去响应诱导抗性造成的毒害。

     

  • 图  1  MeJA局部处理长白落叶松对落叶松毛虫幼虫体内SOD活性的影响

    数据标注均为平均值±标准差(n=3);同一龄期柱形图上,不同小写字母表示同一龄期不同局部处理浓度之间差异显著(P<0.05); MeJA0.01L、MeJA0.10L、MeJA1.00L分别表示MeJA局部喷施浓度为0.01、0.10和1.00 mmol/L。下同。

    Figure  1.  Effects of local spraying MeJA on the SOD activity of Dendrolimus superans larvae

    Data in the figure are means±SD; different lowercase letters in the same age bar chart mean there is significant difference among varied local spraying concentrations in the same instar (P < 0.05). MeJA0.01L, MeJA0.10L and MeJA1.00L mean the local spraying concentrations of MeJA were 0.01, 0.10 and 1.00 mmol/L, respectively. The same below.

    图  2  MeJA局部处理长白落叶松对落叶松毛虫幼虫体内POD活性的影响

    Figure  2.  Effects of local spraying MeJA on the POD activity of Dendrolimus superans larvae

    图  3  MeJA局部处理长白落叶松对落叶松毛虫幼虫体内ACP活性的影响

    Figure  3.  Effects of local spraying MeJA on the ACP activity of Dendrolimus superans larvae

    图  4  MeJA局部处理长白落叶松对落叶松毛虫幼虫体内AKP活性的影响

    Figure  4.  Effects of local spraying MeJA on the AKP activity of Dendrolimus superans larvae

    图  5  MeJA局部处理长白落叶松对落叶松毛虫幼虫体内GSTs活性的影响

    Figure  5.  Effects of local spraying MeJA on the GSTs activity of Dendrolimus superans larvae

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  • 收稿日期:  2016-09-13
  • 修回日期:  2016-10-20
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