Abstract: Expanding road interference on the surrounding landscape has become more evident. During forest landscape zoning, it takes full account of road interference in order to avoid unnecessary landscape fragmentation. In this study, road space disturbance index, which is a quantitative method was used to evaluate the potential impact of road on forest landscape. Taking the inventory data from Ming Dynasty Tombs Forest Farm as the main data source, the index was used and the spatial distribution of interference from local roads was discussed. Finally, the index was used in functional division of local forest management plan.The results showed that the road interference index used in this study reflected the interference condition in the study area. Furthermore, coniferous forest area was most seriously threated from potential interference (average normalized index was 41.87); the main dominant species of Pinus bungeana was potentially interferenced with the biggest threat (65.92), which needed focus on monitoring attention; It was found that in Spearman rank correlation test, the nearest interference (N) impact on the final index was the most significant (0.99). Overall, the index can provide a fast and efficient technical support theory in landscape protection.