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切根贴膜对农田防护林杨树叶片解剖特征及光合生理特性的影响

陈曦 辛颖 赵雨森

陈曦, 辛颖, 赵雨森. 切根贴膜对农田防护林杨树叶片解剖特征及光合生理特性的影响[J]. 北京林业大学学报, 2017, 39(4): 38-45. doi: 10.13332/j.1000-1522.20160406
引用本文: 陈曦, 辛颖, 赵雨森. 切根贴膜对农田防护林杨树叶片解剖特征及光合生理特性的影响[J]. 北京林业大学学报, 2017, 39(4): 38-45. doi: 10.13332/j.1000-1522.20160406
CHEN Xi, XIN Ying, ZHAO Yu-sen. Effects of root excision mulched with plastic film on leaf anatomical characteristics and photosynthetic characteristics of poplar farmland shelterbelts[J]. Journal of Beijing Forestry University, 2017, 39(4): 38-45. doi: 10.13332/j.1000-1522.20160406
Citation: CHEN Xi, XIN Ying, ZHAO Yu-sen. Effects of root excision mulched with plastic film on leaf anatomical characteristics and photosynthetic characteristics of poplar farmland shelterbelts[J]. Journal of Beijing Forestry University, 2017, 39(4): 38-45. doi: 10.13332/j.1000-1522.20160406

切根贴膜对农田防护林杨树叶片解剖特征及光合生理特性的影响

doi: 10.13332/j.1000-1522.20160406
基金项目: 

林业公益性行业科研专项 201404202

详细信息
    作者简介:

    陈曦,博士生。主要研究方向:防护林体系优化配制。Email: dtlal@163.com  地址: 150040  黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院

    责任作者:

    赵雨森,教授,博士生导师。主要研究方向:水土保持与荒漠化防治。Email: Zhaoys1957@163.com  地址:同上

  • 中图分类号: S727.24

Effects of root excision mulched with plastic film on leaf anatomical characteristics and photosynthetic characteristics of poplar farmland shelterbelts

  • 摘要: 以黑龙江省黑土区农田防护林为研究对象,以不切根贴膜为对照,研究距树干0.5、1、2 m切根贴膜处理对杨树光合生理特性、气孔特征及叶绿体超微结构的影响。结果表明:切根贴膜1个月后的杨树净光合速率与对照相比显著降低,下降幅度随着断根强度的加大而增加;切根贴膜3个月后,杨树净光合速率基本恢复到对照水平。切根贴膜处理后的杨树净光合速率下降时伴随着气孔阻力的升高和胞间CO2浓度的下降,表明杨树净光合速率的下降主要是由气孔限制因子引起的。切根贴膜1个月后的杨树叶片上下表皮气孔密度与对照相比均明显降低,之后不断增加,下表皮气孔密度在切根贴膜2个月后已明显高于对照。切根贴膜1个月后杨树叶绿体超微结构受到明显影响,随着断根强度的加大,叶绿体发生膨胀,有明显质壁分离现象,叶绿体片层模糊,淀粉粒体积变小,数量减少,嗜锇颗粒数量大量增加;切根贴膜3个月后的杨树叶片叶绿体超微结构得到了较好的恢复,距树干1、2 m切根贴膜处理的叶绿体中淀粉粒的数量和体积有明显增加现象。杨树因切根贴膜而导致的叶片结构与功能的损伤是可逆的,杨树通过改变自身的光合生理特性来适应切根贴膜所导致的水分胁迫,叶片气孔形态及叶绿体超微结构都发生了适应性变化,这种变化与断根强度相关。综合分析,距树干0.5、1、2 m处切根贴膜是可行的。

     

  • 图  1  试验设置图

    Figure  1.  Experimental design of poplar shelterbelts

    图  2  切根贴膜处理对杨树叶片气孔特征的影响

    A.处理的上表皮,500×;B.处理的下表皮,500×;C.处理的下表皮,2000×;D.贴膜处理的上表皮(以8月C0.5处理为例);E.贴膜处理的下表皮(以8月C0.5处理为例);F.贴膜处理的下表皮(以8月C0.5处理为例)2000×。

    Figure  2.  Effects of root excision mulched with plastic film treatments on stomatal characteristics of poplar leaves

    A, daxial-CK, 500×; B, baxial-CK, ×500;C, baxial-CK, 2000×; D, daxial-C0.5, 500×(taking treatment in August as an example); E, baxial-C0.5, 500×(taking treatment in August as an example); F, axial-C0.5, 2000×(taking treatment in August as an example).

    图  3  切根贴膜处理对杨树叶片叶绿体超微结构的影响

    A1~A3.7—9月对照处理;B1~B3.7—9月C0.5处理;C1~C3.7—9月C1处理;D1~D3.7—9月C2处理;CP.叶绿体;SG.淀粉粒;N.细胞核;W.细胞壁; O.嗜锇颗粒。

    Figure  3.  Effects of root excision mulched with plastic film treatments on the ultrastructure of chloroplasts of poplar leaves

    A1-A3,control treatment in July to September;B1-B3,C0.5 treatment in July to September;C1-C3,C1 treatment in July to September;D1-D3,C2 treatment in July to September; CP,chloroplasts;SG,starch grain;N,nucleus;W,cell wall; O,osmiophilic granules.

    表  1  切根贴膜处理对杨树叶片光合生理特性的影响

    Table  1.   Effects of root excision mulched with plastic film treatments on photosynthetic characteristics of poplar leaves

    月份
    Month
    处理
    Treatment
    Pn/(μmol·m-2·s-1)GS/(mmol·m-2·s-1)Ci/(μmol·mol-1)Tr/(mmol·m-2·s-1)LsWUE/(μmol·mmol-1)
    7CK17.76±2.22a266.46±35.05a206.80±6.93a6.36±0.60a0.43±0.02b2.79±0.16b
    C0.510.07±0.98d124.72±8.71d199.47±13.84a3.41±0.12d0.45±0.04b2.96±0.23a
    C112.51±1.96c148.52±30.06c187.92±6.12b4.07±0.63c0.48±0.02a3.08±0.10a
    C215.12±0.62b218.62±29.62b203.54±11.45a5.55±0.52b0.43±0.03b2.74±0.19b
    8CK12.57±1.39a184.43±29.15a226.86±21.27a6.21±0.84a0.41±0.06bc2.03±0.23a
    C0.59.69±2.07b130.03±15.85b220.60±22.79a5.55±0.75b0.42±0.04bc1.83±0.20a
    C110.35±2.39b138.09±20.98b212.42±26.63a6.09±0.58a0.44±0.06ab1.72±0.19b
    C211.08±1.23b139.31±19.35b194.16±26.81b6.01±0.53a0.48±0.07a1.93±0.19a
    9CK13.11±1.10a271.17±21.54a255.79±18.80a7.40±1.77a0.32±0.04a1.84±0.16a
    C0.512.05±1.62a234.24±23.05b253.65±21.47a6.27±1.28b0.32±0.03a1.98±0.17a
    C112.13±1.42a217.43±24.80c246.70±26.14a6.77±1.93ab0.35±0.03a1.86±0.09a
    C212.31±1.01a229.24±19.62bc250.64±26.30a7.59±1.39a0.34±0.02a1.65±0.11b
    注:7、8、9月份分别为切根贴膜后的第1、2、3个月, 数据为平均值±标准差,同一月份中同列不同字母代表处理间差异显著(P<0.05)。下同。Notes:July, August and September are the 1st, 2nd and 3rd month after root excision, respectively; Data represent mean standard ± deviation, different letters in the same month and column indicate significant difference among treatments at P<0.05 level. The same as below.
    下载: 导出CSV

    表  2  切根贴膜处理对杨树叶片气孔特征参数的影响

    Table  2.   Effects of root excision mulched with plastic film treatments on stomatal characteristic parameters of poplar leaves

    月份
    Month
    处理
    Treatment
    气孔密度/(个·mm-2)
    Density/(number·mm-2)
    气孔长度Stomata length/μm气孔宽度Stomata width/μm
    上表皮Adaxial下表皮Abaxial上表皮Adaxial下表皮Abaxial上表皮Adaxial下表皮Abaxial
    7CK19.3±1.9a136.9±24.4a24.2±3.1a27.9±3.9a15.1±2.8a16.3±3.2a
    C0.59.7±1.7b125.2±17.9b23.7±3.8a25.2±3.9b14.4±2.9a15.9±3.0a
    C19.5±1.5b125.0±12.5b22.8±3.9a26.8±3.8b14.5±2.4a16.2±3.1a
    C28.3±1.1b123.2±16.8b23.6±3.5a27.2±4.0ab14.4±2.6a15.8±3.1a
    8CK22.0±2.0a157.4±7.6b25.0±3.2a24.4±4.1a14.8±2.3a15.2±2.9a
    C0.517.3±1.1b174.9±10.2a25.6±3.5a22.4±4.8c15.2±2.3a14.6±2.7b
    C117.3±1.2b186.0±26.8a26.0±4.1a23.0±3.9bc15.3±2.5a14.6±2.7b
    C216.0±2.3b168.2±8.7a24.8±4.0a23.6±4.0b15.6±2.1a14.9±2.9ab
    9CK33.2±3.0a151.0±4.6b24.2±4.2a23.3±3.9a14.0±2.4a14.4±2.8a
    C0.526.6±1.2b168.8±25.8a23.2±2.1a21.0±4.0c14.9±2.0a13.3±2.6b
    C129.9±2.0ab168.2±8.7a23.5±2.7a21.6±3.6bc14.5±2.5a13.4±2.3b
    C231.2±1.1a173.6±7.1a22.9±4.3a22.0±3.4b14.0±2.4a13.5±2.6b
    下载: 导出CSV
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  • 收稿日期:  2016-12-14
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