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银灰杨花粉母细胞减数分裂与微管骨架变化

李燕杰 田梦迪 刘燕 张媛 张平冬

李燕杰, 田梦迪, 刘燕, 张媛, 张平冬. 银灰杨花粉母细胞减数分裂与微管骨架变化[J]. 北京林业大学学报, 2017, 39(5): 9-16. doi: 10.13332/j.1000-1522.20160417
引用本文: 李燕杰, 田梦迪, 刘燕, 张媛, 张平冬. 银灰杨花粉母细胞减数分裂与微管骨架变化[J]. 北京林业大学学报, 2017, 39(5): 9-16. doi: 10.13332/j.1000-1522.20160417
LI Yan-jie, TIAN Meng-di, LIU Yan, ZHANG Yuan, ZHANG Ping-dong. Meiosis and organization of microtubule of pollen mother cells in Populus canescens[J]. Journal of Beijing Forestry University, 2017, 39(5): 9-16. doi: 10.13332/j.1000-1522.20160417
Citation: LI Yan-jie, TIAN Meng-di, LIU Yan, ZHANG Yuan, ZHANG Ping-dong. Meiosis and organization of microtubule of pollen mother cells in Populus canescens[J]. Journal of Beijing Forestry University, 2017, 39(5): 9-16. doi: 10.13332/j.1000-1522.20160417

银灰杨花粉母细胞减数分裂与微管骨架变化

doi: 10.13332/j.1000-1522.20160417
基金项目: 

国家自然科学基金项目 31570646

详细信息
    作者简介:

    李燕杰。主要研究方向:林木细胞遗传学与多倍体育种。Email : 18637384883@163.com   地址:100083  北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    张平冬,博士,副教授。主要研究方向:林木细胞遗传学与多倍体育种。Email :zhangpd@bjfu.edu.cn  地址:同上

  • 中图分类号: S718.46;S792.11

Meiosis and organization of microtubule of pollen mother cells in Populus canescens

  • 摘要: 减数分裂是植物有性生殖过程中配子形成的必要阶段,是生殖生物学研究领域的热点。本文以银灰杨雄花枝为研究材料,运用醋酸洋红染色法和间接免疫荧光技术,开展银灰杨花粉母细胞减数分裂及其微管骨架动态变化研究。结果表明:1)银灰杨花药颜色变化可作为初步判别其花粉母细胞减数分裂时期的形态学标记,花粉母细胞从减数分裂细线期发育至四分体时期,花药颜色从嫩绿色,经由黄绿色、微红色、浅红色、鲜红色转为深红色。2)银灰杨花粉母细胞减数分裂末期Ⅰ可观察到与着丝粒微管紧密相连的落后染色体,由于染色体两侧着丝粒微管的均衡牵引而致使其停滞于细胞板中央,不移向两极而被遗弃,造成配子染色体的不平衡,可能是导致银灰杨花粉部分败育的机制。3)银灰杨的胞质分裂属于典型的连续型胞质分裂类型,受辐射状微管系统的调节,从细胞周沿向心扩展,直至细胞质完全分离。4)银灰杨花粉中存在天然2n花粉,其发生可能与减数分裂中期Ⅱ的平行纺锤体和三极纺锤体有关。本研究首次报道了银灰杨花药颜色变化与其花粉母细胞减数分裂进程的关系,从新的角度阐释了银灰杨落后染色体的形成原因及其花粉败育机制,并探索了银灰杨天然2n花粉的发生机理,为银灰杨遗传改良策略的制定奠定了基础。

     

  • 图  1  银灰杨花药颜色变化与减数分裂进程的对应关系

    a.嫩绿色花药;b.细线期;c.偶线期;d.黄绿色花药;e.粗线期;f.双线期;g.终变期;h.微红色花药;i.中期Ⅰ;j.后期Ⅰ;k.末期Ⅰ;l.浅红色花药;m.前期Ⅱ;n.中期Ⅱ;o.鲜红色花药;p.后期Ⅱ;q.末期Ⅱ;r.末期Ⅱ多核仁现象;s.深红色花药;t.四分体时期。

    Figure  1.  Corresponding relationship between anther color change and meiosis process in Populus canescens

    a, peak green anther; b, leptotene; c, synaptene; d, yellow green anther; e, pachytene; f, diplotene; g, diakinesis; h, slight red anther; i, metaphase Ⅰ; j, anaphase Ⅰ; k, telophase Ⅰ; l, light red anther; m, prophase Ⅱ; n, metaphase Ⅱ; o, fresh red anther; p, anaphase Ⅱ; q, telophase Ⅱ; r, multi-nucleoli phenomena at telophase Ⅱ; s, crimson anther; t, tetrad.

    图  2  银灰杨花粉母细胞减数分裂过程中微管骨架的动态变化

    a.微管蛋白间接免疫荧光染色;b.染色体DAPI染色;1.减数分裂前期;2.减数分裂终变期;3.中期Ⅰ;4.后期Ⅰ;5.末期Ⅰ;6.末期Ⅰ的落后染色体(箭头所示)7.前期Ⅱ;8.中期Ⅱ;9.后期Ⅱ;10.末期Ⅱ。

    Figure  2.  Dynamic changes of microtubule cytoskeleton during meiosis of pollen mother cells in P. canescens

    a, microtubule protein staining by indirect immunofluorescence; b, chromosomes staining by DAPI; 1, prophase of meiosis; 2, diakinesis; 3, metaphase Ⅰ; 4, anaphase Ⅰ; 5, telophase Ⅰ; 6, lagging chromosomes at telophase Ⅰ(arrow); 7, prophase Ⅱ; 8, metaphase Ⅱ; 9, anaphase Ⅱ; 10, telophase Ⅱ.

    图  3  银灰杨花粉母细胞减数分裂中期Ⅱ纺锤体类型及败育花粉

    a.平行纺锤体;b.垂直纺锤体;c.三极纺锤体;d.败育花粉(箭头所示)。

    Figure  3.  Types of spindle at meiotic metaphase Ⅱ and abortive pollen in P. canescens

    a, parallel spindle; b, vertical spindle; c, tripolar spindle; d, aborted pollen (arrow).

    图  4  银灰杨花粉母细胞细胞质分裂与微管骨架变化

    a.减数分裂末期Ⅰ未见细胞质分裂;b.末期Ⅱ辐射状微管系统调控细胞质向心分裂;c.四分体;d.三分体;e.二分体;f.天然2n花粉(箭头所示)。

    Figure  4.  Cytokinesis of pollen mother cell and microtubule cytoskeleton changes in P. canescens

    a, no cytoplasmic division in telophase Ⅰ; b, nuclear-based radial microtubule regulating sytoplasm centripetal division in telophase Ⅱ; c, tetrad; d, triad; e, dyad; f, natural 2n pollen(arrow).

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  • 收稿日期:  2016-12-29
  • 修回日期:  2017-03-03
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