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胡杨PeAPY1和PeAPY2调控拟南芥耐盐机制研究

邓佳音 张艳丽 张一南 赵瑞 李金克 周晓阳 刘香芬 陈少良

邓佳音, 张艳丽, 张一南, 赵瑞, 李金克, 周晓阳, 刘香芬, 陈少良. 胡杨PeAPY1和PeAPY2调控拟南芥耐盐机制研究[J]. 北京林业大学学报, 2017, 39(6): 13-21. doi: 10.13332/j.1000-1522.20170034
引用本文: 邓佳音, 张艳丽, 张一南, 赵瑞, 李金克, 周晓阳, 刘香芬, 陈少良. 胡杨PeAPY1和PeAPY2调控拟南芥耐盐机制研究[J]. 北京林业大学学报, 2017, 39(6): 13-21. doi: 10.13332/j.1000-1522.20170034
DENG Jia-yin, ZHANG Yan-li, ZHANG Yi-nan, ZHAO Rui, LI Jin-ke, ZHOU Xiao-yang, LIU Xiang-fen, CHEN Shao-liang. PeAPY1 and PeAPY2 of Populus euphratica regulating salt tolerance in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2017, 39(6): 13-21. doi: 10.13332/j.1000-1522.20170034
Citation: DENG Jia-yin, ZHANG Yan-li, ZHANG Yi-nan, ZHAO Rui, LI Jin-ke, ZHOU Xiao-yang, LIU Xiang-fen, CHEN Shao-liang. PeAPY1 and PeAPY2 of Populus euphratica regulating salt tolerance in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2017, 39(6): 13-21. doi: 10.13332/j.1000-1522.20170034

胡杨PeAPY1和PeAPY2调控拟南芥耐盐机制研究

doi: 10.13332/j.1000-1522.20170034
基金项目: 

北京市自然科学基金项目 6172024

教育部科学技术研究(科学技术类) 113013A

教育部创新团队发展计划项目 IRT13047

高等学校学科创新引智计划项目 111project

高等学校学科创新引智计划项目 B130007

国家自然科学基金项目 31270654

国家自然科学基金项目 31570587

详细信息
    作者简介:

    邓佳音:主要研究方向:树木逆境生理。Email:dengjiayin12345@163.com  地址: 100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    陈少良,教授,博士生导师。主要研究方向:树木逆境生理。Email:lschen@bjfu.edu.cn  地址:同上

  • 中图分类号: S792.11;Q945

PeAPY1 and PeAPY2 of Populus euphratica regulating salt tolerance in Arabidopsis thaliana

  • 摘要: 本文研究了胡杨apyrase基因(PeAPY1和PeAPY2)对植物耐盐性的影响。以PeAPY1和PeAPY2过表达拟南芥、拟南芥Atapy1和Atapy2突变体、野生型(WT)拟南芥及空载体(VC)为实验材料,研究盐胁迫条件下的植物根长、相对电导率、细胞活力、H2O2水平、eATP浓度、抗氧化酶活性的变化。研究结果显示:低盐浓度(50 mmol/L NaCl)对拟南芥各基因型的生长和生理生化指标没有显著影响;而高盐浓度(100 mmol/L NaCl)抑制了各株系的根长生长、细胞活力和抗氧化酶(超氧化物歧化酶/SOD、抗坏血酸过氧化酶/APX、过氧化氢酶/CAT)活性,却提高了相对电导率、H2O2水平和eATP浓度。但与Atapy突变体株系相比较,PeAPY过表达株系受高盐胁迫的影响较小。主要是由于PeAPY1/2的过表达提高了apyrase酶活,下调了eATP浓度及其诱发的活性氧水平,同时,过表达株系还通过保持抗氧化酶活性来抑制H2O2的水平,从而降低活性氧及其对膜脂的过氧化,保持了膜的稳定性、细胞活力和生长,最终提高了植物耐盐性。

     

  • 图  1  NaCl对PeAPY过表达株系、Atapy突变体、空载体、野生型拟南芥生长的影响

    A. NaCl对PeAPY过表达株系(PeAPY1-OEPeAPY2-OE)、Atapy突变体(Atapy1、Atapy2)、空载体(VC)与野生型(WT)拟南芥根长生长的影响; B. PeAPY过表达株系、Atapy突变体、空载体与野生型拟南芥在盐胁迫下的根长平均值。每个数值均为3次重复实验的平均值, 每次实验统计25株, 误差线为标准误差(SE); 柱形图上标注的不同字母表示同一盐浓度下不同株系之间存在显著性差异(P<0.05);N. S表示同一处理条件下各基因型之间无显著差异。下同。

    Figure  1.  Effects of NaCl on root growth of PeAPY-transgenic plants, Atapy mutants, vector control and wild-type arabidopsis

    A, effects of NaCl on root growth of PeAPY-transgenic plants (PeAPY1-OE, PeAPY2-OE), Atapy mutants (Atapy1, Atapy2), vector control (VC) and wild-type (WT) arabidopsis; B, average root length of PeAPY-transgenic plants, arabidopsis Atapy mutants, VC and wild-type under salt stress. Each column shows the mean of three independent experiments. Bars represent the standard error of the mean. Columns labelled with different letters show significant differences (P <0.05) between the WT and PeAPY-transgenic lines; N. S, no significant difference. The same below.

    图  2  NaCl对PeAPY过表达株系、Atapy突变体、空载体、野生型拟南芥生长的影响

    Figure  2.  Effects of NaCl on relative electrolyte leakage of PeAPY-transgenic plants (PeAPY1-OE, PeAPY2-OE), Atapy mutants (Atapy1, Atapy2), vector control (VC) and wild-type (WT) arabidopsis

    图  3  NaCl对PeAPY过表达株系、Atapy突变体、空载体、野生型拟南芥细胞活力的影响

    Figure  3.  Effects of NaCl on cell viability of PeAPY-transgenic plants (PeAPY1-OE, PeAPY2-OE), Atapy mutants (Atapy1, Atapy2), vector control (VC) and wild-type (WT) arabidopsis

    图  4  NaCl对PeAPY过表达株系、Atapy突变体、空载体、野生型拟南芥胞外ATP的影响

    Figure  4.  Effects of NaCl on extracellular ATP of PeAPY-transgenic plants (PeAPY1-OE, PeAPY2-OE), Atapy mutants (Atapy1, Atapy2), vector control (VC) and wild-type (WT) arabidopsis

    图  5  NaCl对PeAPY过表达、Atapy突变体、空载体、野生型拟南芥根细胞内源H2O2水平的影响

    Figure  5.  Effects of NaCl on endogenous H2O2 of PeAPY-transgenic plants (PeAPY1-OE, PeAPY2-OE), Atapy mutants (Atapy1, Atapy2), vector control (VC) and wild-type (WT) arabidopsis

    图  6  NaCl对PeAPY过表达株系、Atapy突变体、空载体、野生型拟南芥抗氧化酶APX、CAT和SOD酶活力的影响

    Figure  6.  Effects of NaCl on antioxidase APX (A), CAT (B) and SOD (C) of PeAPY-transgenic plants (PeAPY1-OE, PeAPY2-OE), Atapy mutants (Atapy1, Atapy2), vector control (VC) and wild-type (WT) arabidopsis

    图  7  胡杨apyrase (PeAPY1和PeAPY2)调控拟南芥耐盐性的信号通路

    Figure  7.  A schematic model showing the role of Populus euphratica apyrase (PeAPY1 and PeAPY2) in salt tolerance in Arabidopsis thaliana

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  • 收稿日期:  2017-02-12
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