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杀线虫真菌Sr18发酵液对松材线虫超微结构的影响

王修清 王倩 王亚萍 冯欣 孙建华 孟庆恒

王修清, 王倩, 王亚萍, 冯欣, 孙建华, 孟庆恒. 杀线虫真菌Sr18发酵液对松材线虫超微结构的影响[J]. 北京林业大学学报, 2017, 39(7): 69-75. doi: 10.13332/j.1000-1522.20170048
引用本文: 王修清, 王倩, 王亚萍, 冯欣, 孙建华, 孟庆恒. 杀线虫真菌Sr18发酵液对松材线虫超微结构的影响[J]. 北京林业大学学报, 2017, 39(7): 69-75. doi: 10.13332/j.1000-1522.20170048
WANG Xiu-qing, WANG Qian, WANG Ya-ping, FENG Xin, SUN Jian-hua, MENG Qing-heng. Effects of nematicidal filamentous fungus Sr18 metabolites on the ultrastructure of Bursaphelenchus xylophilus[J]. Journal of Beijing Forestry University, 2017, 39(7): 69-75. doi: 10.13332/j.1000-1522.20170048
Citation: WANG Xiu-qing, WANG Qian, WANG Ya-ping, FENG Xin, SUN Jian-hua, MENG Qing-heng. Effects of nematicidal filamentous fungus Sr18 metabolites on the ultrastructure of Bursaphelenchus xylophilus[J]. Journal of Beijing Forestry University, 2017, 39(7): 69-75. doi: 10.13332/j.1000-1522.20170048

杀线虫真菌Sr18发酵液对松材线虫超微结构的影响

doi: 10.13332/j.1000-1522.20170048
基金项目: 

国家自然科学基金项目 31272019

详细信息
    作者简介:

    王修清。主要研究方向:植物线虫的生物防治。Email:clearmoon2008@126.com  地址:300387  天津市西青区宾水西道延长线393号天津师范大学主校区生命科学学院

    责任作者:

    孟庆恒,副教授。主要研究方向:应用微生物。Email:jbv3139@163.com  地址:同上

  • 中图分类号: S763.3; S432.4+5

Effects of nematicidal filamentous fungus Sr18 metabolites on the ultrastructure of Bursaphelenchus xylophilus

  • 摘要: Sr18真菌是一株自主分离获得的具有广谱杀线虫活性的生防菌。为探明该菌的杀线虫作用机理,以松材线虫为靶标,借助场发射高分辨扫描电镜及透射电镜,对1/2浓度的Sr18发酵液小分子活性组分处理后的线虫及未处理的正常线虫进行了超微观察。高分辨率扫描电镜观察结果表明,小分子物质对松材线虫头部、体壁和尾部均有损伤,并观察到内容物外溢的现象。透射电镜观察结果显示,线虫表皮和体腔分离,细胞核受损严重,形状不规则,粗面内质网核糖体聚集,线粒体空泡样变,肌纤维受损。这些现象从形态结构角度证实了该发酵液小分子活性成分对线虫的损伤是整体性的毒杀作用,排除了表面触杀的机制。这为进一步揭示Sr18的杀线虫机理以及线虫生防制剂的开发提供了重要理论依据。

     

  • 图  1  线虫虫体低倍观察结果

    A.对照(Bar=100 μm);B.处理3天后的线虫(Bar=200 μm)。

    Figure  1.  Ultrastructure observation of Bursaphelenchus xylophilus at low magnification

    A, normal Bursaphelenchus xylophilus(Bar = 100 μm); B, 3 days treated Bursaphelenchus xylophilus by Sr18(Bar = 200 μm).

    图  2  处理后线虫体表SEM观察结果

    A.处理1天后线虫体表出现孔洞(Bar=4 μm);B.放大后的孔洞(Bar=1 μm);C.处理3天后内溶物由孔洞溢出(←)(Bar=1 μm)。

    Figure  2.  Ultrastructure observation of the Bursaphelenchus xylophilu's body surface at high magnification

    A, holes on body surface of nematode treated 1 day by Sr18(Bar=4 μm); B, magnifying hole on body surface(Bar=1 μm); C, dissolved substance inside the cell flew out from the holes after 3 days treated(Bar=1 μm).

    图  3  线虫头部SEM观察结果

    A.对照线虫头部及体壁的细菌(Bar=20 μm);B.对照线虫头部(Bar=3 μm);C.处理3天后萎缩的线虫头部(Bar=1 μm)。

    Figure  3.  Ultrastructure observation of the Bursaphelenchus xylophilu's head at high magnification

    A, head and bacteria on body wall of normal nematode(Bar=20 μm); B, head of normal nematode(Bar=3 μm); C, shrinking head of nematode treated 3 days by Sr18(Bar=1 μm).

    图  4  线虫尾部SEM观察结果

    A.对照线虫尾部及交合刺(Bar=20 μm);B.处理2天后线虫尾部缢缩,肿胀物形成(Bar=20 μm);C.肿胀物(Bar=5 μm)。

    Figure  4.  Ultrastructure observation of the Bursaphelenchus xylophilu's tail at high magnification

    A, tail and spicule of normal nematode(Bar=20 μm); B, shrinking tail and swellings of nematode treated 2 days by Sr18(Bar=20 μm); C, magnifying swelling on body surface(Bar=5 μm).

    图  5  线虫内部结构透射电镜(TEM)观察结果

    A.对照线虫肌纤维(M)及线粒体(MI)(Bar=500 nm);B.正常的细胞核(n)(Bar=500 nm);C.处理1天后的细胞核(n)及内质网(ER)(Bar=500 nm);D.处理2天后的体壁(↘)及细胞损伤(Bar=500 nm);E.处理3天后体壁分离(Bar=2 μm);F.处理3天后体壁分离后组织细胞结构消失(Bar=2 μm)。

    Figure  5.  Ultrastructure observation of the Bursaphelenchus xylophilu's internal structure at high magnification

    A, muscle fiber and mitochondria of normal nematode(Bar=500 nm); B, cell nucleus of normal nematode(Bar=500 nm); C, damaged cell nucleus and endoplasmic reticulum of nematode treated 1 day by Sr18(Bar=500 nm); D, swellings on body wall and cellular damage of nematode treated 2 days by Sr18(Bar=500 nm); E, separation of cuticle and coelom of nematode treated 3 days by Sr18 (Bar=2 μm); F, disappearance of tissue and cell structure after separation of nematode treated 3 days by Sr18 (Bar=2 μm).

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  • 收稿日期:  2017-02-22
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