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外源BR与IAA对欧美杨耐旱性的影响

贾伏丽 王丛鹏 刘沙 焦志银 尹伟伦 夏新莉

贾伏丽, 王丛鹏, 刘沙, 焦志银, 尹伟伦, 夏新莉. 外源BR与IAA对欧美杨耐旱性的影响[J]. 北京林业大学学报, 2017, 39(7): 31-39. doi: 10.13332/j.1000-1522.20170055
引用本文: 贾伏丽, 王丛鹏, 刘沙, 焦志银, 尹伟伦, 夏新莉. 外源BR与IAA对欧美杨耐旱性的影响[J]. 北京林业大学学报, 2017, 39(7): 31-39. doi: 10.13332/j.1000-1522.20170055
JIA Fu-li, WANG Cong-peng, LIU Sha, JIAO Zhi-yin, YIN Wei-lun, XIA Xin-li. Effects of exogenous BR and IAA on drought tolerance of Populus deltoides × P.nigra[J]. Journal of Beijing Forestry University, 2017, 39(7): 31-39. doi: 10.13332/j.1000-1522.20170055
Citation: JIA Fu-li, WANG Cong-peng, LIU Sha, JIAO Zhi-yin, YIN Wei-lun, XIA Xin-li. Effects of exogenous BR and IAA on drought tolerance of Populus deltoides × P.nigra[J]. Journal of Beijing Forestry University, 2017, 39(7): 31-39. doi: 10.13332/j.1000-1522.20170055

外源BR与IAA对欧美杨耐旱性的影响

doi: 10.13332/j.1000-1522.20170055
基金项目: 

“十二五”国家科技支撑计划项目 2015BAD07B01

国家自然科学基金项目 31270656

详细信息
    作者简介:

    贾伏丽。主要研究方向:植物抗逆分子生物学。Email: Fulijia1219@163.com  地址:100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    尹伟伦,教授,博士生导师。主要研究方向:植物生理与生物技术。Email: yinwl@bjfu.edu.cn  地址:同上

    夏新莉,教授,博士生导师。主要研究方向:植物抗逆分子生物学。Email: xiaxl@bjfu.edu.cn  地址:同上

  • 中图分类号: S718.43;S792.119

Effects of exogenous BR and IAA on drought tolerance of Populus deltoides × P.nigra

  • 摘要: 为了探究外源油菜素类固醇(brassinosteroid,BR)和IAA对杨树耐旱性的影响,本文选取3种欧美杨无性系NE19、R270、107的1年生盆栽苗木,分别喷洒10 μmol/L BR和100 mg/L IAA,并同时控制土壤含水量为20%,对其进行为期15 d的干旱处理和非干旱处理,对它们的生长和生理变化进行检测。结果表明:与无激素组相比,BR与IAA均可显著提高干旱条件下3种欧美杨无性系的净光合速率(Pn)、光化学量子产量(Fv/Fm)、叶绿素含量、叶片保水能力。BR处理NE19、R270、107的Pn比非干旱组分别提高19%、60%、85%,IAA处理分别高了35%、50%、80%;对Fv/Fm,BR处理的NE19、R270、107比对照组提高了7.7%、7.5%、10.9%,而IAA组提高了7.4%、8%、11.9%。以上结果说明外源施加BR与IAA提高了欧美杨无性系的耐旱性。统计经过激素处理后的欧美杨无性系气孔指数发现,BR处理NE19、R270、107的气孔指数分别下降12.28%、25.60%、20.31%,IAA处理分别下降13.00%、13.15%、14.48%,因此我们推测施加BR和IAA,会抑制植物气孔发育以降低干旱条件下的水分消耗;通过荧光定量PCR,检测与气孔发育6个相关基因EPF1、EPF2、EPFL9、ERECTAFAMASDD1的表达量特征,发现BR和IAA处理导致负调节气孔发育相关基因EPF1、EPF2、ERECTASDD1表达量升高,正调节气孔发育基因FAMAEPFL9表达量下降。综上所述,植物激素BR与IAA参与欧美杨气孔发育过程,在干旱条件下通过抑制气孔发育提高欧美杨抗旱性。

     

  • 图  1  不同水分处理条件下3个无性系的光合速率

    A、B.土壤相对含水量50%; C、D.土壤相对含水量20%。图中数据为平均值±标准误差(n=3)。下同。

    Figure  1.  Variation of photosynthetic rate of 3 clones under different water treatments

    A, B, soil relative water content is 50%; C, D, soil relative water content is 20%; data are mean ± SD (n=3). NE-19, Populus nigra×(P. deltoides×P. nigra); R270, P. deltoides ×P. nigra, 107, Peuramericana cv.'74/76'. The same below.

    图  2  不同水分处理条件下外源IAA和BR对3种欧美杨光化学量子产量(Fv/Fm)的影响

    Figure  2.  Effects of exogenous IAA and BR on the photochemical quantum yield (Fv/Fm) of three clones under different water treatments

    图  3  不同水分处理条件下外源IAA和BR对3个无性系欧美杨叶绿素含量的影响

    A~C.3种无性系叶绿素a含量;D~F.3种无性系叶绿素b含量。

    Figure  3.  Effects of exogenous IAA and BR on chlorophyll content of three clones under different water treatments

    A-C, chlorophyll a content of three clones; D-F, chlorophyll b content of three clones.

    图  4  外源施加IAA与BR对欧美杨叶片相对含水量的影响

    Figure  4.  Effects of exogenous IAA and BR on leaf relative water content of Populus deltoides × P. nigra

    图  5  外源施加IAA与BR对3个欧美杨无性系气孔导度(Gs)的影响

    Figure  5.  Effects of exogenous IAA and BR onstomatal conductance of three clones

    图  6  IAA与BR降低欧美杨幼叶气孔密度

    Figure  6.  IAA and BR reducing the stomatal density of Populus deltoides × P. nigra

    图  7  IAA与BR处理下气孔发育相关基因表达变化

    Figure  7.  Transcriptional abundance of different stomatal development-related genes compared with hormone-free group

    表  1  各处理组详情表

    Table  1.   Treatment group in detail

    土壤相对含水量
    Soil relative water content
    20%50%
    NE19Hormone free-NE19Hormone free-NE19
    IAA-NE19IAA-NE19
    BR-NE19BR-NE19
    R270Hormone free-R270Hormone free-R270
    IAA- R270IAA- R270
    BR- R270BR- R270
    107Hormone free-107Hormone free-107
    IAA-107IAA-107
    BR-107BR-107
    下载: 导出CSV

    表  2  荧光定量PCR引物

    Table  2.   Primers for quantitative real-time RT-PCR

    基因名称Gene name正向引物Forward primer反向引物Reverse primer
    UBQAGACCTACACCAAGCCCAAGAAGATCCAGCACCGCACTCAGCATTAG
    Potri.019G102800ATGCTCACCATGTAGACTAGGGACAGGATAAGACTTGTTATG
    Potri.013G116600GGAAGCAGCAAGAGAAGATGAAGGCACATGGTAGTATT
    Potri.002G249900CACCACCTGCTCATAAGTTGACATCTGCTCTTCATTGC
    Potri.013g136100TCTCTGTCTTCTTCCAATGGAGTCTCAGCCTCTTCAAGT
    ERECTAATCCAGGGCTGATGACAACAACAGTAATGCAAGTTGGAAA
    FAMAATCAGTGCCAAGCTTGAAGAAACACAGGGCAGTTGCTTCC
    SDD1AATATCATGTCAGGATCATCTTGTCACTAACATAGGCAGT
    下载: 导出CSV

    表  3  3个无性系各处理间气孔指数对比

    Table  3.   Variation of stomatal index among 3 clone treatments

    无激素
    Hormone-free
    IAABRIAA组降低率
    Reducing rate of IAA treatment/%
    BR组降低率
    Reducing rate of BR treatment/%
    NE1916.61+0.85a14.45+0.45b14.57+0.96b13.0012.28
    R27020.00+0.99a17.37+0.22b14.88+0.7c13.1525.60
    10718.51+1.3a15.83+0.71b14.75+0.89b14.4820.31
    注:表中数据为平均值±标准误差(n=3),不同小写字母代表差异显著(P<0.05)。Notes: data are mean ± SD (n=3).Different letters indicate significant difference (P<0.05).
    下载: 导出CSV
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  • 收稿日期:  2017-03-06
  • 修回日期:  2017-04-19
  • 刊出日期:  2017-07-01

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