Extraction technology of anthocyanin from Lonicera caerulea 'Beilei' fruit by ultrasonic-assisted countercurrent extraction
-
摘要: 在单因素实验的基础上，采用响应面法优化超声辅助逆流提取技术提取蓓蕾蓝靛果花色苷工艺，结果表明，超声辅助逆流提取蓓蕾花色苷的最佳工艺为：逆流提取级数为3级，提取时间、温度和功率分别为28 min、30 ℃和120 W。此最佳工艺条件下，花色苷得率为(5.23±0.023) mg/g，提取率达到99.80%，与超声波法比较(89.86%)，该工艺提取率提高了10%。处理相同批量的原料，与常规超声波提取相比，该工艺可节省3级提取溶剂67%，节约时间44.4%。Abstract: The response surface methodology based on single factor experiment was used to optimize processing parameters of extraction of Lonicera caerulea 'Beilei' anthocyanin by ultrasonic-assisted countercurrent extraction. The results showed that the optimal processing conditions of ultrasonic-assisted countercurrent extraction were ultrasonic time 28 min, extraction temperature 30 ℃ and ultrasonic power 120 W, the countercurrent extraction stage was three levels. The yields of anthocyanin under the optimal extraction conditions were up to (5.23±0.023) mg/g, the extraction rate was 99.80%, which was increased by 10% compared with ultrasonic extraction method. For the same batch of raw materials, compared with conventional ultrasonic extraction, this process can save three extraction solvent 67% and save time 44.4%.
-
-
![]()
图 1 4级逆流提取原理示意图
数字1~22表示提取顺序；A、B、C、D表示4个提取单元；P1、P2、P3、P4表示4个提取阶段；圆形表示蓓蕾蓝靛果原料；n1~n7表示新提取剂的编号；·表示花色苷物质的含量。
Figure 1. Schematic diagram of four-stage countercurrent extraction principle
No.1 to 22 indicate the extraction order; A, B, C, and D represent four extraction units; P1, P2, P3, P4 represent four extraction stages; the circular indicates the raw material of Lonicera caerulea 'Beilei'; n1-n7 indicate the number of the new extraction solvent; ·means the content of anthocyanin.
![]()
图 2 不同超声温度下的花色苷得率
不同字母表示差异显著(P＜0.05)。下同。
Figure 2. Anthocyanin yield under different ultrasonic temperatures
Different letters indicate significant differences(P < 0.05). The same below.
表 1 响应面试验因素水平设计表
Table 1 Factors and levels of response surface experiments
因素Factor 编码水平Coding level 时间Time (A) -1(20) 0(30) 1(40) 温度Temperature (B) -1(25) 0(30) 1(35) 功率Power (C) -1(105) 0(120) 1(135) 
下载: 导出CSV
表 2 3因素响应面试验设计表
Table 2 Results of response surface experiments
处理号
Test code时间
Time(A)温度
Temperature(B)功率
Power(C)花色苷得率
Anthocyanin yield(Y)/(mg·g-1)1 0 0 0 4.71 2 1 1 0 3.19 3 -1 0 1 4.09 4 0 1 -1 3.03 5 1 0 1 3.78 6 0 1 1 3.13 7 -1 0 -1 4.06 8 1 0 -1 3.42 9 0 0 0 4.79 10 -1 -1 0 3.39 11 1 -1 1 3.16 12 0 0 0 4.81 13 -1 1 0 3.05 14 0 0 0 4.75 15 0 -1 1 2.97 16 0 0 0 4.73 17 0 -1 -1 3.42
下载: 导出CSV
表 3 蓓蕾蓝靛果花色苷提取工艺的显著性方差分析结果
Table 3 Results of variance analysis for regression model
方差来源
Source of variancedf 平方和
Sum of squares均方
Mean squaresF P R2 模型Model 9 8.11 0.90 37.42 0.000 1** 0.979 6 时间Time (A) 1 0.14 0.14 5.61 0.049 7* 温度Temperature (B) 1 0.036 0.036 1.51 0.258 3 功率Power (C) 1 2.000×10-4 2.000×10-4 8.305×10-3 0.929 9 AB 1 0.034 0.034 1.42 0.272 1 AC 1 0.027 0.027 1.13 0.323 0 BC 1 0.076 0.076 3.14 0.119 7 A2 1 0.78 0.78 32.36 0.000 7** B2 1 5.38 5.38 223.35 0.000 1** C2 1 1.01 1.01 42.02 0.000 3** 残差Residual 7 0.17 0.024 31.34 失拟Lack of fit 3 0.16 0.154 0.107 纯误差Pure error 4 6.880×10-3 1.720×10-3 总离差Cor total 16 8.28 注：**表示在P＜0.01水平上差异显著，*表示在P＜0.05水平上差异显著。Notes: ** means significant difference at P＜0.01 level, * means significant difference at P＜0.05 level.
下载: 导出CSV
表 4 蓓蕾蓝靛果花色苷逆流提取效果
Table 4 Countercurrent extraction effect of anthocyanins of Lonicera caerulea 'Beilei'
提取级数(级)
Extraction stage(stage)逆流提取溶剂用量
Countercurrent extraction solvent content/mL超声提取溶剂用量
Ultrasonic extraction solvent content/mL逆流提取时间
Countercurrenttime/min超声提取时间
Ultrasonic time/min花色苷得率
Anthocyanin yield/(mg·g-1)花色苷提取率
Anthocyanin extraction rate/%单级Single stage 20 20 40 40 4.69±0.035a 89.69 2级Two stages 40 80 120 160 4.92±0.035b 93.89 3级Three stages 60 180 200 360 5.22±0.023c 99.80 4级Four stages 80 320 240 640 5.24±0.026c 100.00
下载: 导出CSV
-
[1] 秦栋, 霍俊伟, 睢薇.蓝果忍冬品种蓓蕾蓝靛果引种与选育[J].中国果树, 2011 (5): 37-39. QIN D, HUO J W, SUI W. Introduction and breeding of Lonicera Caerulea Beilei[J]. China Fruits, 2011 (5): 37-39.
[2] 顾文秀, 谢为明, 夏文水, 等.超声波提取乌饭树叶色素及其稳定性的研究[J].林产化学与工业, 2005, 25 (4): 74-78. doi: 10.3321/j.issn:0253-2417.2005.04.018 GU W X, XIE W M, XIA W S, et al. Ultrasonic extraction of pigment from leaves of Vaccinium bracteatum Thumb.[J]. Chemistry and Industry of Forest Products, 2005, 25 (4): 74-78. doi: 10.3321/j.issn:0253-2417.2005.04.018
[3] 时玉晴, 苏东民, 苏东海, 等.超声波辅助法提取黑色小麦麸皮花色苷工艺优化[J].粮食与油脂, 2015, 28(4): 15-18. doi: 10.3969/j.issn.1008-9578.2015.04.005 SHI Y Q, SU D M, SU D H, et al. Optimization of extraction process for black wheat bran anthocyanins by ultrasonic-assisted extraction[J]. Grain and Oil, 2015, 28(4): 15-18. doi: 10.3969/j.issn.1008-9578.2015.04.005
[4] 程秀玮, 魏玮.响应面优化超声波辅助提取桑葚花色苷工艺的研究[J].中国酿造, 2014, 33 (6): 123-127. http://d.old.wanfangdata.com.cn/Periodical/zgnz201406030 CHENG X W, WEI W. Optimization of ultrsonic extraction technology of melberry anthocyanin by response surface methodology[J]. China Brewing, 2014, 33 (6): 123-127. http://d.old.wanfangdata.com.cn/Periodical/zgnz201406030
[5] 李金星, 胡志和, 马立志, 等.超声波辅助提取蓝莓果渣中花色苷的条件研究[J].食品工业科技, 2013, 34 (20): 255-259. http://d.old.wanfangdata.com.cn/Periodical/spgykj201320058 LI J X, HU Z H, MA L Z, et al. Study on conditions of anthocyanins from blueberry pomace by ultrasonic-assisted extraction[J]. Science and Technology of Food Industry, 2013, 34 (20): 255-259. http://d.old.wanfangdata.com.cn/Periodical/spgykj201320058
[6] 李希, 谢守德, 冯建安, 等.动态逆流提取技术在中药提取中的应用[J].实用中医药杂志, 2008, 24(12): 806-808. doi: 10.3969/j.issn.1004-2814.2008.12.050 LI X, XIE S D, FENG J A, et al. Application of dynamic countercurrent extraction technology in extraction of traditional chinese medicine[J]. Journal of Practical Traditional Chinese Medicine, 2008, 24(12): 806-808. doi: 10.3969/j.issn.1004-2814.2008.12.050
[7] 张毅, 宁正祥, 董华强, 等.多穗柯总黄酮的连续逆流提取研究[J].现代食品科技, 2011, 27(5): 550-552, 570. http://d.old.wanfangdata.com.cn/Periodical/gzspgykj201105016 ZHANG Y, NING Z X, DONG H Q, et al. Continuous countercurrent extraction of flavonoids from Lithocarpus polysachyus Rehd.[J]. Modern Food Science and Technology, 2011, 27(5): 550-552, 570. http://d.old.wanfangdata.com.cn/Periodical/gzspgykj201105016
[8] 王巧娥, 任虹, 曹学丽.甘草中甘草酸的多级逆流提取技术研究[J].北京工商大学学报(自然科学版), 2011, 29(1): 33-37. http://d.old.wanfangdata.com.cn/Periodical/bjqgyxyxb201101008 WANG Q E, REN H, CAO X L. Multistage countercurrent extraction techniques for extraction of glycyrrhizic acid from licorice[J]. Journal of Beijing Technology and Business University(Natural Science Edition), 2011, 29(1): 33-37. http://d.old.wanfangdata.com.cn/Periodical/bjqgyxyxb201101008
[9] 栾连军, 陈娜, 刘雪松, 等.葡萄籽原花青素多级逆流提取工艺研究[J].中国食品学报, 2010, 10(5): 31-36. doi: 10.3969/j.issn.1009-7848.2010.05.005 LUAN L J, CHEN N, LIU X S, et al. Studies on the multi-stage countercurrent extraction technology for the extraction of proanthocaynidins from grape seed[J]. Journal of Chinese Institute of Food Science and Technology, 2010, 10(5): 31-36. doi: 10.3969/j.issn.1009-7848.2010.05.005
[10] 韩丽, 谢秀琼, 杨明, 等.丹参动态阶段连续逆流提取工艺研究[J].中成药, 2009, 31(5): 721-724. doi: 10.3969/j.issn.1001-1528.2009.05.020 HAN L, XIE X Q, YANG M, et al. Study on continuous extraction of Salvia miltiorrhiza[J]. Chinese Traditional Patent Medicine, 2009, 31(5): 721-724. doi: 10.3969/j.issn.1001-1528.2009.05.020
[11] 王艳艳, 王团结, 陈娟.连续动态逆流提取技术及其设备研究[J].机电信息, 2015(5): 1-9, 14. doi: 10.3969/j.issn.1671-0797.2015.05.001 WANG Y Y, WANG T J, CHEN J. Study on continuous dynamic countercurrent extraction technology and equipment[J]. Electromechanical Information, 2015(5): 1-9, 14. doi: 10.3969/j.issn.1671-0797.2015.05.001
[12] 焦岩, 王振宇.蓝靛果花色苷对高脂膳食诱导肥胖大鼠脂代谢和抗氧化能力的影响[J].食品科学, 2010, 31(3): 230-234. http://d.old.wanfangdata.com.cn/Periodical/spkx201003053 JIAO Y, WANG Z Y. Effect of Lonicera caerulea anthocyanin on lipid metabolism and antioxidant function in high fat diet-induced obese rats[J]. Food Science, 2010, 31(3): 230-234. http://d.old.wanfangdata.com.cn/Periodical/spkx201003053
[13] ZHAO H T, WANG Z Y, MA F M, et al. Protective effect of anthocyanin from Lonicera caerulea var. edulis on radiation-induced damage in mice[J]. International Journal of Molecular Sciences, 2012, 13(9): 11773-11782. http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_88d2239bb7a9e123238cc1e70313f610
[14] 王恩福, 包怡红.蓝靛果提取物调节血脂功能特性的研究[J].食品与机械, 2010, 26(1): 81-82. http://d.old.wanfangdata.com.cn/Periodical/spyjj201001023 WANG E F, BAO Y H. Study on blood lipid adjusting effect of Lonicera edulis extracts[J]. Food & Machinery, 2010, 26(1): 81-82. http://d.old.wanfangdata.com.cn/Periodical/spyjj201001023
[15] 谢志鹏, 刘雪松, 陈勇, 等.动态罐组式逆流提取技术在中药生产中的应用研究进展[J].中国中药杂志, 2007, 32(10): 884-887. doi: 10.3321/j.issn:1001-5302.2007.10.003 XIE Z P, LIU X S, CHEN Y, et al. Advances in studies on multi-stage countercurrent extraction technology in traditional Chinese medicine[J]. China Journal of Chinese Materia Medica, 2007, 32(10): 884-887. doi: 10.3321/j.issn:1001-5302.2007.10.003
[16] 蔡铭, 陈勇, 刘雪松, 等.丹参中丹参素四效逆流提取工艺的优化[J].中国中药杂志, 2006, 31(18): 1499-1502. doi: 10.3321/j.issn:1001-5302.2006.18.005 CAI M, CHEN Y, LIU X S, et al. Optmizing process of four-effect countercurrent extraction of dan shen su from Salvia miltiorrhiza[J]. China Journal of Chinese Materia Medica, 2006, 31(18): 1499-1502. doi: 10.3321/j.issn:1001-5302.2006.18.005
[17] 周丽萍, 杨瑞华, 张悦.响应面法优化研磨珠辅助超声波萃取苹果多酚的工艺研究[J].食品工业科技, 2012, 33(21): 277-281. http://d.old.wanfangdata.com.cn/Periodical/spgykj201221081 ZHOU L P, YANG R H, ZHANG Y. Optimization of apple polyphenols extraction with grind balls by response surface methodology[J]. Science and Technology of Food Industry, 2012, 33(21): 277-281. http://d.old.wanfangdata.com.cn/Periodical/spgykj201221081
[18] 陈钢, 黄立山, 徐静, 等.黑莓花色苷的超声波提取与抗氧化性活性研究[J].食品科学, 2012, 33(24): 117-121. doi: 10.7506/spkx1002-6630-201224024 CHEN G, HUANG L S, XU J, et al. Ultrasonic-assisted extraction and antioxidant activity of anthocyanins from blackberry fruits[J]. Food Science, 2012, 33(24): 117-121. doi: 10.7506/spkx1002-6630-201224024
[19] 张帅, 张少平, 郑云云, 等.红凤菜花色苷三种提取方法的工艺优化和比较[J/OL].食品工业科技, 2017[2017-03-15].http://kns.cnki.net/kcms/detail/11.1759.TS.20170307.1418.016.html. ZHANG S, ZHANG S P, ZHENG Y Y, et al. Optimization and comparison of three extraction methods of anthocyanin from Gynura bicolor[J/OL]. Science and Technology of Food Industry, 2017[2017-03-15]. http://kns.cnki.net/kcms/detail/11.1759.TS.20170307.1418.016.html.
[20] 王巧娥, 任虹, 曹学丽.动态逆流提取技术及其在天然活性成分提取中的应用[J].安徽农业科学, 2011, 39(5): 2535-2537, 2540. doi: 10.3969/j.issn.0517-6611.2011.05.006 WANG Q E, REN H, CAO X L. Dynamic countercurrent extraction technology and its application in extracting natural active constituents[J]Journal of Anhui Agricultural Sciences, 2011, 39(5): 2535-2537, 2540. doi: 10.3969/j.issn.0517-6611.2011.05.006
[21] 陈红专, 王曙宾, 高鹏, 等.人参超声逆流提取工艺研究[J].中草药, 2012, 43 (6): 1115-1117. http://d.old.wanfangdata.com.cn/Periodical/zcy201206019 CHEN H Z, WANG S B, GAO P, et al. Study on ultrasound countercurrent extraction technology of Panax ginseng[J]. Chinese Traditional and Herbal Drugs, 2012, 43(6): 1115-1117. http://d.old.wanfangdata.com.cn/Periodical/zcy201206019
-
期刊类型引用(1)
1. 卜弘毅，朱正杰，崔素珍，张明，李成之，许志敏，李典鹏，曹学城，孙天一，雷礼纲. 洪泽湖不同湿地类型表层土壤与沉积物碳氮含量特征分析. 湿地科学与管理. 2025(01): 45-51 . 
百度学术
其他类型引用(0)
计量
- 文章访问数: 2172
- HTML全文浏览量: 356
- PDF下载量: 30
- 被引次数: 1
