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超声波辅助逆流提取蓓蕾蓝靛果花色苷工艺

周丽萍 王化 何丹娆 杜凤国

周丽萍, 王化, 何丹娆, 杜凤国. 超声波辅助逆流提取蓓蕾蓝靛果花色苷工艺[J]. 北京林业大学学报, 2017, 39(9): 119-125. doi: 10.13332/j.1000-1522.20170119
引用本文: 周丽萍, 王化, 何丹娆, 杜凤国. 超声波辅助逆流提取蓓蕾蓝靛果花色苷工艺[J]. 北京林业大学学报, 2017, 39(9): 119-125. doi: 10.13332/j.1000-1522.20170119
ZHOU Li-ping, WANG Hua, HE Dan-rao, DU Feng-guo. Extraction technology of anthocyanin from Lonicera caerulea 'Beilei' fruit by ultrasonic-assisted countercurrent extraction[J]. Journal of Beijing Forestry University, 2017, 39(9): 119-125. doi: 10.13332/j.1000-1522.20170119
Citation: ZHOU Li-ping, WANG Hua, HE Dan-rao, DU Feng-guo. Extraction technology of anthocyanin from Lonicera caerulea 'Beilei' fruit by ultrasonic-assisted countercurrent extraction[J]. Journal of Beijing Forestry University, 2017, 39(9): 119-125. doi: 10.13332/j.1000-1522.20170119

超声波辅助逆流提取蓓蕾蓝靛果花色苷工艺

doi: 10.13332/j.1000-1522.20170119
基金项目: 

国家重点研发计划项目 2016YFC500300

黑龙江省科学院对外合作基金项目“蓝靛果花色苷抗肝癌功能研究” 2016

黑龙江省科学院研究基金项目“蓝靛果花色苷抗氧化功能及其构效关系研究” 2014

详细信息
    作者简介:

    周丽萍,博士生,副研究员。主要研究方向:植物活性成分。Email:zhoulipingnefu@163.com  地址:100540 黑龙江省哈尔滨市香坊区哈平路103号黑龙江省科学院自然与生态研究所

    责任作者:

    杜凤国,教授,博士生导师。主要研究方向:植物资源培育与开发利用。Email:dfg4656@qq.com  地址:132013  吉林省吉林市滨江东路3999号北华大学林学院

  • 中图分类号: S759.82

Extraction technology of anthocyanin from Lonicera caerulea 'Beilei' fruit by ultrasonic-assisted countercurrent extraction

  • 摘要: 在单因素实验的基础上,采用响应面法优化超声辅助逆流提取技术提取蓓蕾蓝靛果花色苷工艺,结果表明,超声辅助逆流提取蓓蕾花色苷的最佳工艺为:逆流提取级数为3级,提取时间、温度和功率分别为28 min、30 ℃和120 W。此最佳工艺条件下,花色苷得率为(5.23±0.023) mg/g,提取率达到99.80%,与超声波法比较(89.86%),该工艺提取率提高了10%。处理相同批量的原料,与常规超声波提取相比,该工艺可节省3级提取溶剂67%,节约时间44.4%。

     

  • 图  1  4级逆流提取原理示意图

    数字1~22表示提取顺序;A、B、C、D表示4个提取单元;P1、P2、P3、P4表示4个提取阶段;圆形表示蓓蕾蓝靛果原料;n1~n7表示新提取剂的编号;·表示花色苷物质的含量。

    Figure  1.  Schematic diagram of four-stage countercurrent extraction principle

    No.1 to 22 indicate the extraction order; A, B, C, and D represent four extraction units; P1, P2, P3, P4 represent four extraction stages; the circular indicates the raw material of Lonicera caerulea 'Beilei'; n1-n7 indicate the number of the new extraction solvent; ·means the content of anthocyanin.

    图  2  不同超声温度下的花色苷得率

    不同字母表示差异显著(P<0.05)。下同。

    Figure  2.  Anthocyanin yield under different ultrasonic temperatures

    Different letters indicate significant differences(P < 0.05). The same below.

    图  3  不同超声时间下的花色苷得率

    Figure  3.  Anthocyanin yield under different ultrasonic time

    图  4  超声功率对花色苷得率的影响

    Figure  4.  Effects of ultrasonic power on anthocyanin yield

    图  5  响应曲面图

    Figure  5.  Response surface

    图  6  正态分布图

    Figure  6.  Normal plot of residuals

    表  1  响应面试验因素水平设计表

    Table  1.   Factors and levels of response surface experiments

    因素Factor编码水平Coding level
    时间Time (A)-1(20)0(30)1(40)
    温度Temperature (B)-1(25)0(30)1(35)
    功率Power (C)-1(105)0(120)1(135)
    下载: 导出CSV

    表  2  3因素响应面试验设计表

    Table  2.   Results of response surface experiments

    处理号
    Test code
    时间
    Time(A)
    温度
    Temperature(B)
    功率
    Power(C)
    花色苷得率
    Anthocyanin yield(Y)/(mg·g-1)
    10004.71
    21103.19
    3-1014.09
    401-13.03
    51013.78
    60113.13
    7-10-14.06
    810-13.42
    90004.79
    10-1-103.39
    111-113.16
    120004.81
    13-1103.05
    140004.75
    150-112.97
    160004.73
    170-1-13.42
    下载: 导出CSV

    表  3  蓓蕾蓝靛果花色苷提取工艺的显著性方差分析结果

    Table  3.   Results of variance analysis for regression model

    方差来源
    Source of variance
    df平方和
    Sum of squares
    均方
    Mean squares
    FPR2
    模型Model98.110.9037.420.000 1**0.979 6
    时间Time (A)10.140.145.610.049 7*
    温度Temperature (B)10.0360.0361.510.258 3
    功率Power (C)12.000×10-42.000×10-48.305×10-30.929 9
    AB10.0340.0341.420.272 1
    AC10.0270.0271.130.323 0
    BC10.0760.0763.140.119 7
    A210.780.7832.360.000 7**
    B215.385.38223.350.000 1**
    C211.011.0142.020.000 3**
    残差Residual70.170.02431.34
    失拟Lack of fit30.160.1540.107
    纯误差Pure error46.880×10-31.720×10-3
    总离差Cor total168.28
    注:**表示在P<0.01水平上差异显著,*表示在P<0.05水平上差异显著。Notes: ** means significant difference at P<0.01 level, * means significant difference at P<0.05 level.
    下载: 导出CSV

    表  4  蓓蕾蓝靛果花色苷逆流提取效果

    Table  4.   Countercurrent extraction effect of anthocyanins of Lonicera caerulea 'Beilei'

    提取级数(级)
    Extraction stage(stage)
    逆流提取溶剂用量
    Countercurrent extraction solvent content/mL
    超声提取溶剂用量
    Ultrasonic extraction solvent content/mL
    逆流提取时间
    Countercurrenttime/min
    超声提取时间
    Ultrasonic time/min
    花色苷得率
    Anthocyanin yield/(mg·g-1)
    花色苷提取率
    Anthocyanin extraction rate/%
    单级Single stage202040404.69±0.035a89.69
    2级Two stages40801201604.92±0.035b93.89
    3级Three stages601802003605.22±0.023c99.80
    4级Four stages803202406405.24±0.026c100.00
    下载: 导出CSV
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  • 收稿日期:  2017-04-07
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