超声波辅助逆流提取蓓蕾蓝靛果花色苷工艺

Extraction technology of anthocyanin from Lonicera caerulea 'Beilei' fruit by ultrasonic-assisted countercurrent extraction

摘要: 在单因素实验的基础上，采用响应面法优化超声辅助逆流提取技术提取蓓蕾蓝靛果花色苷工艺，结果表明，超声辅助逆流提取蓓蕾花色苷的最佳工艺为：逆流提取级数为3级，提取时间、温度和功率分别为28 min、30 ℃和120 W。此最佳工艺条件下，花色苷得率为(5.23±0.023) mg/g，提取率达到99.80%，与超声波法比较(89.86%)，该工艺提取率提高了10%。处理相同批量的原料，与常规超声波提取相比，该工艺可节省3级提取溶剂67%，节约时间44.4%。

Abstract: The response surface methodology based on single factor experiment was used to optimize processing parameters of extraction of Lonicera caerulea 'Beilei' anthocyanin by ultrasonic-assisted countercurrent extraction. The results showed that the optimal processing conditions of ultrasonic-assisted countercurrent extraction were ultrasonic time 28 min, extraction temperature 30 ℃ and ultrasonic power 120 W, the countercurrent extraction stage was three levels. The yields of anthocyanin under the optimal extraction conditions were up to (5.23±0.023) mg/g, the extraction rate was 99.80%, which was increased by 10% compared with ultrasonic extraction method. For the same batch of raw materials, compared with conventional ultrasonic extraction, this process can save three extraction solvent 67% and save time 44.4%.