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不同世代樟子松育种资源遗传评价

苗禹博 朱晓梅 李志娟 贾凤岭 李伟

苗禹博, 朱晓梅, 李志娟, 贾凤岭, 李伟. 不同世代樟子松育种资源遗传评价[J]. 北京林业大学学报, 2017, 39(12): 71-78. doi: 10.13332/j.1000-1522.20170194
引用本文: 苗禹博, 朱晓梅, 李志娟, 贾凤岭, 李伟. 不同世代樟子松育种资源遗传评价[J]. 北京林业大学学报, 2017, 39(12): 71-78. doi: 10.13332/j.1000-1522.20170194
MIAO Yu-bo, ZHU Xiao-mei, LI Zhi-juan, JIA Feng-ling, LI Wei. Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations[J]. Journal of Beijing Forestry University, 2017, 39(12): 71-78. doi: 10.13332/j.1000-1522.20170194
Citation: MIAO Yu-bo, ZHU Xiao-mei, LI Zhi-juan, JIA Feng-ling, LI Wei. Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations[J]. Journal of Beijing Forestry University, 2017, 39(12): 71-78. doi: 10.13332/j.1000-1522.20170194

不同世代樟子松育种资源遗传评价

doi: 10.13332/j.1000-1522.20170194
基金项目: 

国家重点研发计划项目 2017YFD0600505

详细信息
    作者简介:

    苗禹博。主要研究方向:针叶树遗传改良。Email:MIAO_Yubo@163.com   地址:100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    李伟,博士,教授。主要研究方向:针叶树遗传改良。Email: bjfuliwei@bjfu.edu.cn   地址:同上

  • 中图分类号: S722.3+3

Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations

  • 摘要: 当前樟子松遗传改良正处于升级换代的关键时期,但是不同地区育种资源调拨与交换频繁,导致了育种资源信息模糊、亲缘关系混乱,增加了樟子松遗传改良工作中近交衰退的风险,因此开展育种资源评价研究是樟子松优良单株利用的重要基础。本文以樟子松1、1.5、2代种子园224个无性系为研究对象,开展了基于SSR的遗传多样性和单株亲缘关系分析以及遗传差异分析。结果表明:按育种资源的来源分析,良种基地、头道桥、榆林1.5和泰来4个群体的遗传多样性水平较高,宝根园和榆林群体相对较低;按育种资源的世代分析,不同世代樟子松育种资源维持着较高的遗传多样性水平,高世代种子园的遗传多样性水平有所下降,2、1.5代种子园的期望杂合度He较1代种子园分别下降4.13%、1.31%。2代种子园群体的近交系数F值(0.173)较高,纯合子较多,显著偏离了哈迪-温伯格平衡。同种源、不同生境的樟子松育种资源没有发生明显的变异,主要的遗传变异存在于个体之间(96%);随着种子园世代的提高,无性系之间的亲缘关系聚类现象逐渐明显,2代种子园内存在两组完全一致的无性系(CH3、CH10组和TL1417、TL1411组)。研究结果完善了樟子松育种资源评价体系,确定了不同世代育种资源的亲缘关系,为樟子松高级遗传改良工作提供了材料和信息基础。

     

  • 图  1  樟子松1代(a)、1. 5代(b)和2代(c)种子园无性系亲缘关系

    Figure  1.  Phylogenetic relationship tree of the 1st(a), 1. 5th(b) and 2nd(c) seed orchards

    表  1  实验材料信息

    Table  1.   Experimental material information

    种子园
    Seed orchard
    材料来源
    Material source
    数量
    Quantity
    1st 良种基地LiangZhongJiDi(LZJD) 57
    头道桥TouDaoQiao(TOU) 15
    宝根园BaoGenYuan(BAO) 11
    巴日图BaRiTu(BA) 11
    红林场HongLinChang(HONG) 14
    1.5th 榆林YuLin1.5(YL1.5) 28
    2nd 错海CuoHai(CH) 34
    泰来TaiLai(TL) 33
    榆林YuLin2(YL2) 21
    下载: 导出CSV

    表  2  樟子松SSR多态性引物

    Table  2.   Polymorphic SSR primers of Pinus sylvestris var. mongolica

    编号
    NO.
    位点
    Locus
    引物序列
    Primer sequence (5′ -3′)
    退火温度
    Annealing
    temperature/℃
    重复基元
    Repeat motif
    片段大小
    Fragment
    size/bp
    多态信息含量
    Polymorphism information
    content (PIC)
    1 lw_isotig05123 F:TGTGCGTATAGGAGGTGGAG
    R:ATGAAAGGTGACAAAGCGGT
    55 (GAG)6 180~186 0.184
    2 lw_isotig07383 F:CAAACAAAAAACAGTCTGCA R:ATCGTCATCATCATCGTCAC 55(GAT)8 198~2160.555
    3 lw_isotig26230 F:GGGCATTACATAAACACGGG R:TGCCCTTGAGCATTTGATTA 55 (TA)10 263~2910.393
    4 lw_isotig02138 F:ATGCATCTTGTCCTCTCT
    R:TTCCTGATTCACACTCCC
    42 (AG)6 135~143 0.200
    5 lw_isotig01420 F:TCCGTGACCCTATTACGT R:CGATTAGTTGCTTGCCTT 50 (CTG)5 184~196 0.520
    6 lw_isotig04306 F:GCCATTTTTTTCTTCTCTCCT R:GGTCGGTTTCTGAATTTCTAA 55 (TCC)7 197~215 0.555
    7 lw_isotig04204 F:CTCCGTTTGGGTTGTGTTTG R:ATCCTTGCCGCCAGATTTGT 55 (CGGCT)5 243~263 0.291
    8 lw_isotig11166 F:ACACACACTGAGCTCCAATTT R:AGTCCCACCTCTGCTGATACA 55 (TA)7 160~170 0.715
    9 lw_isotig10603 F:CAAAATCGTCTACTTCTCCCCC R:CAAAGCAAAAGAACTCCAACGA 55 (CAG)7 208~223 0.443
    10 lw_isotig21953 F:ATGGTGTGTTTGAAGCGGAA R:ATTGCAGCCACTGGTGTCTT 55 (ATGGG)7 217~309 0.831
    11 lw_isotig20215 F:AGAGGTGATCGCAGTCAAAGA
    CAAAAAGACCAAACCGTAG
    55 (TA)7 194~212 0.665
    下载: 导出CSV

    表  3  不同地区樟子松群体遗传多样性水平

    Table  3.   Genetic diversity level of Pinus sylvestris var. mongolica populations in different regions

    种子园
    Seed orchard
    群体
    Population
    观测等位基因
    Observe number of
    allele (Na)
    有效等位基因
    Effectivenumber
    of allele (Ne)
    Shannon多样性指数
    Shannon’s
    diversity
    index (I)
    观测杂合度
    Nei’s observed
    heterozygosity
    (Ho)
    期望杂合度
    Nei’sexpected
    heterozygosity
    (He)
    LZJD 4.909 2.519 0.995 0.453 0.528
    TOU 4.091 2.705 1.002 0.505 0.533
    1st BAO 3.636 2.549 0.890 0.417 0.470
    BA 3.636 2.376 0.910 0.455 0.498
    HONG 4.000 2.576 0.975 0.536 0.522
    1.5th YL1.5 4.818 2.600 1.000 0.488 0.511
    CH 4.636 2.446 0.963 0.425 0.499
    2nd TL 4.364 2.611 0.995 0.484 0.534
    YL2 3.818 2.312 0.879 0.388 0.467
    下载: 导出CSV

    表  4  不同世代樟子松种子园遗传多样性

    Table  4.   Genetic diversity of seed orchards in different generations of Pinus sylvestris var. mongolica

    位点
    编号
    Locus
    No.
    观测等位基因
    Observe number of
    allele (Na)
    有效等位基因
    Effective number
    of allele (Ne)
    基因型数
    Number of genotype
    观测杂合度
    Nei’s observed
    heterozygosity (Ho)
    期望杂合度
    Nei’s expected
    heterozygosity (He)
    Shannon多样性指数
    Shannon’s
    diversity
    index (I)
    多态信息含量
    Polymorphism
    information content(PIC)
    近交系数
    Inbreeding
    coefficient (F)
    1 3/3/3 1.182/1.241/1.342 4/3/4 0.130/0.214/0.273 0.154/0.195/0.255 0.316/0.389/0.459 0.145/0.181/0.228 0.159/-0.102/-0.064
    2 5/5/7 2.218/2.387/2.531 11/5/13 0.463/0.321/0.409 0.549/0.581/0.605 1.072/1.123/1.286 0.509/0.530/0.576 0.157/0.447/0.329
    3 3/3/3 1.991/2.013/1.923 5/5/4 0.287/0.357/0.125 0.498/0.503/0.480 0.771/0.815/0.715 0.399/0.420/0.375 0.423/0.290/0.742
    4 4/2/2 1.379/1.074/1.174 7/2/2 0.292/0.071/0.161 0.275/0.069/0.148 0.511/0.154/0.280 0.274/0.067/0.137 -0.064/-0.037/-0.082
    5 5/5/4 2.337/2.644/2.245 9/7/7 0.639/0.643/0.625 0.572/0.622/0.555 1.032/1.144/0.992 0.513/0.555/0.500 -0.117/-0.034/-0.121
    6 6/5/4 2.477/2.469/2.849 10/7/9 0.611/0.821/0.659 0.596/0.595/0.649 1.093/1.052/1.183 0.528/0.511/0.591 -0.025/-0.380/-0.01
    7 2/2/2 1.693/1.280/1.453 2/2/2 0.574/0.250/0.386 0.409/0.219/0.312 0.599/0.377/0.491 0.326/0.195/0.263 -0.403/-0.143/-0.234
    8 6/6/6 3.807/4.000/4.260 17/12/16 0.519/0.679/0.648 0.737/0.750/0.765 1.498/1.522/1.564 0.696/0.712/0.727 0.297/0.095/0.159
    9 5/4/4 2.192/2.253/2.088 7/6/7 0.593/0.464/0.523 0.544/0.556/0.521 0.900/0.939/0.858 0.446/0.465/0.430 -0.090/0.165/0.002
    10 11/10/14 6.487/5.809/6.156 37/17/33 0.704/0.778/0.779 0.846/0.828/0.838 2.043/1.959/2.071 0.828/0.808/0.819 0.168/0.060/0.076
    11 10/8/8 3.113/3.431/2.967 24/11/16 0.340/0.333/0.232 0.679/0.709/0.663 1.536/1.439/1.346 0.690/0.664/0.610 0.499/0.530/0.654
    总计
    Total
    5.455/4.818/5.182 2.625/2.600/2.635 12.091/7.000/10.273 0.468/0.448/0.438 0.533/0.511/0.526 1.034/1.000/1.022 0.487/0.464/0.478 0.091/0.081/0.173
    注:“/”左边、中间、右边分别代表:1、1.5、2代园的遗传参数。Notes:the genetic parameters of the1st, 1.5th and 2nd seed orchards were on the left, middle and right.
    下载: 导出CSV

    表  5  樟子松分子方差分析与固定指数

    Table  5.   Analysis of molecular variance and fixation index of Pinus sylvestris var. mongolica

    种子园Seed orchard 变异来源Source of variation 自由度Degree of freedom 方差Variance 均方差Mean variance 估计方差Est. Var. 变异系数Coefficient of variation/% 固定指数Fixation index(Fst)
    1st 群体间Among populations 4 51.633 3.908 0.015 0
    群体内个体间Among individuals within population 103 347.849 3.377 0.408 14 0.041
    群体间个体间Among individuals between populations 108 276.500 2.560 2.560 86
    总计Total 215 639.981 2.983 100
    2nd 群体间Among populations 2 22.060 11.030 0.134 4
    群体内个体间Among individuals within population 85 282.713 3.326 0.467 16 0.045
    群体间个体间Among individuals between populations 88 210.500 2.392 2.392 80
    总计Total 175 515.273 2.993 100
    下载: 导出CSV
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  • 收稿日期:  2017-06-21
  • 修回日期:  2017-10-19
  • 刊出日期:  2017-12-01

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