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黄檗幼苗抗氧化物质及生物碱含量对UV-B辐射增强响应

张玉红 程楷珊 高欣 张锡国 刘彤

张玉红, 程楷珊, 高欣, 张锡国, 刘彤. 黄檗幼苗抗氧化物质及生物碱含量对UV-B辐射增强响应[J]. 北京林业大学学报, 2018, 40(1): 27-36. doi: 10.13332/j.1000-1522.20170201
引用本文: 张玉红, 程楷珊, 高欣, 张锡国, 刘彤. 黄檗幼苗抗氧化物质及生物碱含量对UV-B辐射增强响应[J]. 北京林业大学学报, 2018, 40(1): 27-36. doi: 10.13332/j.1000-1522.20170201
Zhang Yu-hong, Cheng Kai-shan, Gao Xin, Zhang Xi-guo, Liu Tong. Influence on antioxidants and alkaloid content of Phellodendron amurense seedlings grown under supplementary UV-B radiation[J]. Journal of Beijing Forestry University, 2018, 40(1): 27-36. doi: 10.13332/j.1000-1522.20170201
Citation: Zhang Yu-hong, Cheng Kai-shan, Gao Xin, Zhang Xi-guo, Liu Tong. Influence on antioxidants and alkaloid content of Phellodendron amurense seedlings grown under supplementary UV-B radiation[J]. Journal of Beijing Forestry University, 2018, 40(1): 27-36. doi: 10.13332/j.1000-1522.20170201

黄檗幼苗抗氧化物质及生物碱含量对UV-B辐射增强响应

doi: 10.13332/j.1000-1522.20170201
基金项目: 

林业公益性行业科研专项 201104066

国家自然科学基金项目 31370630

详细信息
    作者简介:

    张玉红,博士,副教授。主要研究方向:药用植物生理生态学。Email:pzhangyh@126.com 地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学332信箱

  • 中图分类号: S718.43;S792.31

Influence on antioxidants and alkaloid content of Phellodendron amurense seedlings grown under supplementary UV-B radiation

  • 摘要: 目的本文以东北重要药用植物黄檗幼苗为对象, 研究黄檗幼苗中抗氧化物质以及主要药用活性成分含量变化对增补不同的UV-B辐射强度的响应。方法通过在温室内控制实验, 设置了3个UV-B辐射强度梯度的处理, 分别是对照组(CK, 自然光照)、低辐射组(T1, UV-B辐射强度为3.26 μW/cm2)和高辐射组(T2, UV-B辐射强度为9.78 μW/cm2), 研究黄檗幼苗中的抗氧化物质及主要有效活性成分, 即3种生物碱(小檗碱、药根碱和掌叶防已碱)含量对不同UV-B辐射强度的响应。结果增补UV-B辐射会使黄檗幼苗中的羟基自由基、丙二醛、单宁、可溶性蛋白含量明显增加并随辐射强度升高而增加; 除第40天处理外, 不同处理间黄檗幼苗根中总黄酮含量随紫外辐射强度呈下降趋势, 而茎中总黄酮含量随辐射强度增强而升高, 叶中总黄酮含量随辐射强度升高和处理时间延长而升高; 在辐射处理40 d时, 黄檗幼苗茎中3种生物碱含量都高于对照, 低强度辐射的T1组含量高于高强度辐射的T2组且差异显著(P < 0.05)。根和叶中3种生物碱在辐射处理40 d时虽随辐射时间增长含量有所升高但仍低于对照且差异显著(P < 0.05)。结论UV-B辐射增强会导致药用植物黄檗不同器官中的抗氧化物质和药用活性成分含量发生不同的变化, 这对以后黄檗不同利用模式的大规模种植有着重要意义, 同时也对今后的药用植物中生物碱的利用有着深远影响。

     

  • 图  1  不同UV-B处理下黄檗幼苗羟基自由基的含量变化

    不同小写字母表示差异显著,相同小写字母表示差异不显著(P < 0.05)。下同。

    Figure  1.  Variation in hydroxyl radical content of P. amurense seedlings under different UV-B treatments

    Different lowercase letters mean significant difference, same lowercase letters mean no significant difference (P < 0.05). The same below.

    图  2  不同UV-B处理下黄檗叶片中丙二醛的含量变化

    Figure  2.  Variation of MDA content in leaves of P. amurense seedlings under different UV-B treatments

    图  3  不同UV-B处理下黄檗叶片中单宁含量变化

    Figure  3.  Variation of tannin content in leaves of P. amurense seedlings under different UV-B treatments

    图  4  不同UV-B处理黄檗叶片可溶性蛋白含量变化

    Figure  4.  Variation of soluble protein content in leaves of P. amurense seedlings under different UV-B treatments

    图  5  不同UV-B处理黄檗幼苗不同器官总黄酮含量变化

    Figure  5.  Variation of general flavone content in different organs of P. amurense seedlings under different UV-B treatments

    图  6  不同UV-B处理黄檗幼苗中小檗碱含量变化

    Figure  6.  Variation of berberine content in seedlings of P. amurense under different UV-B treatments

    图  7  不同UV-B处理黄檗幼苗中药根碱含量变化

    Figure  7.  Variation of jatrorrhizine content in seedlings of P. amurense under different UV-B treatments

    图  8  不同UV-B处理黄檗幼苗中掌叶防已碱含量变化

    Figure  8.  Variation of palmatine content in seedlings of P. amurense under different UV-B treatments

  • [1] Krupa S V, Kichert R N. The greenhouse effect: impacts of ultraviolet-B (UV-B) radiation, carbon dioxide (CO2), and ozone (O3) on vegetation[J]. Environmental Pollution, 1989, 61(4): 263-393. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1177/097152319400100208
    [2] Callow J A. Advances in botanical research:volume 22 [M]. San Diego: Academic Press, 1996, 22: 97-162.
    [3] Teramura A H. UV-B Effects on terrestrial plants [J]. Photochemistry and Photobiology, 1989, 50(4): 479-487. doi: 10.1111/php.1989.50.issue-4
    [4] 晏斌, 戴秋杰.紫外线B对水稻叶组织中活性氧代谢及膜系统的影响[J].植物生理学报, 1996, 22(4): 373-378. doi: 10.3321/j.issn:1671-3877.1996.04.001

    Yan B, Dai Q J. Effects of ultraviolet-B radiation on active oxygen metabolism and membrane system of rice leaves [J]. Acta Phytophysiologica Sinica, 1996, 22(4): 373-378. doi: 10.3321/j.issn:1671-3877.1996.04.001
    [5] Krause G H. Photoinhibition of photosynthesis: an evaluation of damaging and protective mechanisms [J]. Physiologia Plantarum, 1988, 74(3): 566-574. doi: 10.1111/ppl.1988.74.issue-3
    [6] 薛慧君, 岳明.UV-B辐射增强对陆地植物次生代谢的影响[J].西北植物学报, 2004, 24(6): 1131-1137. doi: 10.3321/j.issn:1000-4025.2004.06.033

    Xue H J, Yue M. Effects of enhanced UV-B radiation on terrestrial plant secondary metabolite [J]. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(6): 1131-1137. doi: 10.3321/j.issn:1000-4025.2004.06.033
    [7] 李方民, 陈怡平, 王勋陵, 等. UV-B辐射增强和CO2浓度倍增的复合作用对番茄生长和果实品质的影响[J].应用生态学报, 2006, 17(1): 71-74. doi: 10.3321/j.issn:1001-9332.2006.01.015

    Li F M, Chen Y P, Wang X L, et al. Combined effects of enhanced UV-B radiation and doubled CO2 on tomato growth and its fruit quality [J]. Chinese Journal of Applied Ecology, 2006, 17(1): 71-74. doi: 10.3321/j.issn:1001-9332.2006.01.015
    [8] 傅立国.中国植物红皮书:稀有濒危植物[M].北京:科学出版社, 1991.

    Fu L G. China plant redbook: rare and endangered plant [M]. Beijing: Science Press, 1991.
    [9] 国家林业局.中国重点保护野生植物资源调查[M].北京:中国林业出版社, 2009: 156-157.

    State Forestry Administration. Investigation on the key protection of wild plant resources in China [M]. Beijing: China Forestry Publishing House, 2009: 156-157.
    [10] 国家药典委员会.中华人民共和国药典:1部[M].北京:中国医药科技出版社, 2010: 137.

    Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic of China: Volume 1[M]. Beijing: China Medicine Press, 2010: 137.
    [11] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社, 2006.

    Gao J F. Experimental guidance for plant physiology [M]. Beijing: Higher Education Press, 2006.
    [12] 李兰芳, 张魁, 吴树勋, 等.不同生长期白羊草中总黄酮的含量测定[J].中国野生植物资源, 1996, 23(4): 37-39. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199600890363

    Li L F, Zhang K, Wu S X, et al. Determination of total flavonoids in different grown period of Bothriochloa ischaemum [J]. Chinese Wild Plant Resources, 1996, 23(4): 37-39. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199600890363
    [13] 彭英丽.磷钾营养对黄檗幼苗生长及主要生物碱含量的影响[D].哈尔滨: 东北林业大学, 2015: 8.

    Peng Y L. Influence of phosphorus and potassium nutrition on growth and alkaloid content of Phellodendren amurense seedlings[D]. Harbin: Northeast Forestry University, 2015: 8.
    [14] Hilal M, Parrado M F, Rosa M, et al. Epidermal lignin deposition in quinoa cotyledons in response to UV-B radiation[J]. Photochemistry and Photobiology, 2004, 79(2): 205-210. doi: 10.1562/0031-8655(2004)079<0205:ELDIQC>2.0.CO;2
    [15] 许大全, 张玉忠, 张荣铣.植物光合作用的光抑制[J].植物生理学通报, 1992, 28(4): 237-243. http://d.old.wanfangdata.com.cn/Periodical/zwxtb200003004

    Xu D Q, Zhang Y Z, Zhang R X. Photoinhibition of photosynthesis in plants [J]. Plant Physiology Communications, 1992, 28(4): 237-243. http://d.old.wanfangdata.com.cn/Periodical/zwxtb200003004
    [16] 张书娜, 王庆成, 郝龙飞, 等.光照和施肥对白桦林冠下水曲柳、胡桃楸苗木生长的影响[J].森林工程, 2015, 31(2): 51-56. doi: 10.3969/j.issn.1001-005X.2015.02.011

    Zhang S N, Wang Q C, Hao L F, et al. Effects of light and fertilization on the growth of Fraxinus mandshurica and Juglans mandshurica seedlings under the canopy of Betula platyphylla secondary forest [J]. Forest Engineering, 2015, 31(2): 51-56. doi: 10.3969/j.issn.1001-005X.2015.02.011
    [17] He J, Huang L K, Chow W S, et al. Effects of supplementary untraviolet-B radiation on rice and pea plants [J]. Australian Journal of Plant Physiology, 1993, 20(2): 129-142.
    [18] 阚光锋, 史翠娟, 缪锦来, 等.南极冰藻Chlamydomonas sp. L4的UV-B辐射效应研究[J].高技术通讯, 2008, 18(9): 966-970. doi: 10.3772/j.issn.1002-0470.2008.09.016

    Kan G F, Shi C J, Miu J L, et al. Effect of UV-B radiation on Antarctic ice microalga Chlamydomonas sp. L4 [J]. Chinese High Technology Letters, 2008, 18(9): 966-970. doi: 10.3772/j.issn.1002-0470.2008.09.016
    [19] 吴杏春, 林文雄, 黄忠良. UV-B辐射增强对两种不同抗性水稻叶片光合生理及超显微结构的影响[J].生态学报, 2007, 27(2): 554-564. doi: 10.3321/j.issn:1000-0933.2007.02.017

    Wu X C, Lin W X, Huang Z L. Influence of enhanced ultraviolet-B radiation on photosynthetic physiologies and ultrastructure of leaves in two different resistivity rice cultivars [J]. Acta Ecologica Sinica, 2007, 27(2): 554-564. doi: 10.3321/j.issn:1000-0933.2007.02.017
    [20] 雷晓强, 王竞红, 杨成武, 等.干旱胁迫下三种护坡植物种子萌发特性研究[J].森林工程, 2015, 31(3): 7-11. doi: 10.3969/j.issn.1001-005X.2015.03.002

    Lei X Q, Wang J H, Yang C W, et al. Study on drought resistance of three woody plants during the seed germination [J]. Forest Engineering, 2015, 31(3): 7-11. doi: 10.3969/j.issn.1001-005X.2015.03.002
    [21] 雷珍, 戴海英, 叶和军, 等.轮叶蒲桃对干旱胁迫的生理响应影响研究[J].森林工程, 2015, 31(5): 4-6, 34. doi: 10.3969/j.issn.1001-005X.2015.05.002

    Lei Z, Dai H Y, Ye H J, et al. Study on the physiological response of Syzygium grijsii to drought stress [J]. Forest Engineering, 2015, 31(5): 4-6, 34. doi: 10.3969/j.issn.1001-005X.2015.05.002
    [22] 刘敏, 李荣贵, 范海, 等.UV-B辐射对烟草光合色素和几种酶的影响[J].西北植物学报, 2007, 27(2): 291-296. doi: 10.3321/j.issn:1000-4025.2007.02.014

    Liu M, Li R G, Fan H, et al. Effects of enhanced uv-b radiation on photosynthetic pigments and some enzymes in tobacco [J]. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(2): 291-296. doi: 10.3321/j.issn:1000-4025.2007.02.014
    [23] 牛传坡, 蒋静艳, 黄耀. UV-B辐射对土壤-冬小麦系统碳氮转化的影响[J].矿物岩石地球化学通报, 2007, 26(S14): 470-471. http://d.old.wanfangdata.com.cn/Periodical/kwysdqhxtb2007z1221

    Niu C P, Jiang J Y, Huang Y. Effects of UV-B radiation on the carbon nitrogen conversion of soil- winter wheat system [J]. Bulletin of Mineralogy, Petrology and geochemistry, 2007, 26(S14): 470-471. http://d.old.wanfangdata.com.cn/Periodical/kwysdqhxtb2007z1221
    [24] Strid A·, Porra R J. Alterations in pigment content in leaves of Pisum sativum after exposure to supplementary UV-B [J]. Plant and Cell Physiology, 1992, 33(7):1015-1023. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HighWire000002647016
    [25] Petropoulou Y, Kyparissis A, Nikolopoulos D, et al. Enhanced UV-B radiation alleviates the adverse effects of summer drought in two Mediterranean pines under field conditions[J]. Physiologia Plantarum, 1995, 94(1): 37-44. doi: 10.1111/ppl.1995.94.issue-1
    [26] Lud D, Moerdijk T C W, Van De Poll W H, et al. DNA damage and photosynthesis in Antarctic and Arctic Sanionia uncinata (Hedw.) Loeske under ambient and enhanced levels of UV-B radiation [J]. Plant Cell & Environment, 2002, 25(12): 1579-1589.
    [27] 梁滨, 周青. UV-B辐射对植物总黄酮影响的研究进展[J].作物学报, 2001, 27(5): 319-323.

    Liang B, Zhou Q. Research progress on the influence of UV-B radiation on total flavonoids in plants [J]. Acta Agronomica Sinica, 2001, 27(5): 319-323.
    [28] Warren J M, Bassman J H, Mattinson D S, et al. Alteration of foliar flavonoid chemistry induced by enhanced UV-B radiation in field-grown Pinus ponderosa, Quercus rubra and Pseudotsuga menziesii [J]. Journal of Photochemistry and Photobiology B: Biology, 2002, 66(2): 125-133. doi: 10.1016/S1011-1344(02)00230-0
    [29] 董新纯, 赵世杰, 郭珊珊, 等.增强UV-B条件下类黄酮与苦荞逆境伤害和抗氧化酶的关系[J].山东农业大学学报(自然科学版), 2006, 37(2): 157-162. doi: 10.3969/j.issn.1000-2324.2006.02.001

    Dong X C, Zhao S J, Guo S S, et al. Role of flavonoids on stress injury and antioxydative enzymes in Fagopyrum tataricum seedlings under enhanced UV-bradiation [J]. Journal of Shandong Agricultural University (Natural Science Edition), 2006, 37(2): 157-162. doi: 10.3969/j.issn.1000-2324.2006.02.001
    [30] 王海霞, 刘文哲.增强UV-B辐射对喜树幼苗生物量和两种生物碱含量的影响[J].植物科学学报, 2011, 29(6): 712-717. http://d.old.wanfangdata.com.cn/Periodical/whzwxyj201106009

    Wang H X, Liu W Z. Effects of enhanced UV-B radiation on biomass and contents of camptothecin and 10-hydroxy-camptothecin in Camptotheca acuminata [J]. Plant Science Journal, 2011, 29(6): 712-717. http://d.old.wanfangdata.com.cn/Periodical/whzwxyj201106009
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  • 收稿日期:  2017-06-12
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