高级检索

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

3种植物次生代谢物质对舞毒蛾的杀虫活性分析

王亚军 邹传山 王若茜 林连男 张国财

王亚军, 邹传山, 王若茜, 林连男, 张国财. 3种植物次生代谢物质对舞毒蛾的杀虫活性分析[J]. 北京林业大学学报, 2017, 39(11): 75-81. doi: 10.13332/j.1000-1522.20170214
引用本文: 王亚军, 邹传山, 王若茜, 林连男, 张国财. 3种植物次生代谢物质对舞毒蛾的杀虫活性分析[J]. 北京林业大学学报, 2017, 39(11): 75-81. doi: 10.13332/j.1000-1522.20170214
WANG Ya-jun, ZOU Chuan-shan, WANG Ruo-xi, LIN Lian-nan, ZHANG Guo-cai. Insecticidal activity analysis of three plant secondary metabolites on Lymantria dispar[J]. Journal of Beijing Forestry University, 2017, 39(11): 75-81. doi: 10.13332/j.1000-1522.20170214
Citation: WANG Ya-jun, ZOU Chuan-shan, WANG Ruo-xi, LIN Lian-nan, ZHANG Guo-cai. Insecticidal activity analysis of three plant secondary metabolites on Lymantria dispar[J]. Journal of Beijing Forestry University, 2017, 39(11): 75-81. doi: 10.13332/j.1000-1522.20170214

3种植物次生代谢物质对舞毒蛾的杀虫活性分析

doi: 10.13332/j.1000-1522.20170214
基金项目: 

黑龙江省森工总局科研项目 sgzjT2015005

中央高校基本科研业务费专项 2572014CA15

哈尔滨市应用技术研究与开发项目 2016RAXXJ035

详细信息
    作者简介:

    王亚军。主要研究方向:森林有害生物综合管理。Email:1404259265@qq.com  地址:150040  黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院

    责任作者:

    张国财,教授,博士生导师。主要研究方向:森林有害生物综合管理。Email:Zhang640308@126.com  地址:同上

  • 中图分类号: S763.42

Insecticidal activity analysis of three plant secondary metabolites on Lymantria dispar

  • 摘要: 为探究苦参碱、氧化苦参碱和山奈酚对舞毒蛾3龄幼虫的杀虫活性以及杀虫机理,本研究通过生物测定、离体和体内酶活性实验,分析了上述3种植物次生代谢物质对舞毒蛾的杀虫效果及对其谷胱甘肽S-转移酶(GST)、乙酰胆碱酯酶(AChE)和羧酸酯酶(CarE)活性的影响。实验结果表明:苦参碱、氧化苦参碱和山奈酚均对舞毒蛾3龄幼虫有良好的杀虫效果,其中苦参碱杀虫效果最强,致死中质量浓度(LC50)为0.420 mg/mL;离体酶活性实验发现,3种植物次生代谢物质对舞毒蛾离体GST、AChE和CarE活性均有抑制效果,其中氧化苦参碱对GST和AChE抑制作用最强,抑制中质量浓度(IC50)分别为0.928和0.717 mg/mL,而苦参碱对CarE抑制作用最强,IC50为0.436 mg/mL;而体内酶活性实验结果显示,苦参碱对GST抑制作用最强,在处理后48 h,GST活性达到最低,仅为同时期对照组的39%;而氧化苦参碱对AChE和CarE抑制作用最强,在处理后72 h,AChE和CarE活性最低,分别为对照组的44%和40%。以上结果表明苦参碱、氧化苦参碱和山奈酚对舞毒蛾幼虫GST、AChE和CarE活性的抑制作用是其具有杀虫活性的原因之一。

     

  • 图  1  3种植物次生代谢物质对舞毒蛾幼虫离体GST、AChE和CarE活性的影响

    Figure  1.  Effects of three plant secondary metabolites on in vitro GST, AChE and CarE activities of L. dispar

    图  2  3种植物次生代谢物质对舞毒蛾幼虫体内GST活性的影响

    柱形图上不同小写字母表示不同药剂处理之间差异显著(P<0.05)。下同。

    Figure  2.  Effects of three plant secondary metabolites on in vivo GST activity of L. dispar

    Different lowercase letters in the same time bar chart mean there is significant difference among varied treatments of plant secondary substances (P < 0.05). The same as below.

    图  3  3种植物次生代谢物质对舞毒蛾幼虫体内AChE活性的影响

    Figure  3.  Effects of three plant secondary metabolites on in vivo AChE activity of L. dispar

    图  4  3种植物次生代谢物质对舞毒蛾幼虫体内CarE活性的影响

    Figure  4.  Effects of three plant secondary metabolites on in vivo CarE activity of L. dispar

    表  1  3种植物次生代谢物质对舞毒蛾3龄幼虫的毒力

    Table  1.   Toxicity of three plant secondary metabolites on 3rd instar larvae of Lymantria dispar

    药剂
    Agent
    回归方程
    Regression equation
    R2 LC50/
    (mg·mL-1)
    95%置信区间
    95% confidence limit
    LC25/
    (mg·mL-1)
    95%置信区间
    95% confidence limit
    苦参碱Matrine y=0.342x+5.299 0.991 0.420 0.311~0.556 0.060 0.035~0.086
    氧化苦参碱Oxymatrine y=0.225x+5.072 0.952 0.730 0.617~0.964 0.040 0.023~0.071
    山奈酚Kaempferol y=0.377x+4.491 0.995 3.860 2.051~5.866 0.650 0.461~0.924
    注:LC50为致死中质量浓度,LC25为亚致死质量浓度。Notes: LC50 means median lethal mass concentration, LC25 means sublethal mass concentration.
    下载: 导出CSV

    表  2  3种植物次生代谢物质对舞毒蛾3龄幼虫离体解毒酶活性的抑制中质量浓度(IC50)

    Table  2.   IC50 values of three plant secondary metabolites to in vitro detoxifying enzymes of 3rd instar L. dispar larvae


    Enzyme
    药剂
    Agent
    IC50/
    (mg·mL-1)
    95%置信区间
    95% confidence limit
    R2
    GST 苦参碱Matrine 1.209±0.144 a 1.011~1.476 0.972
    氧化苦参碱Oxymatrine 1.199±0.153 a 1.039~1.401 0.977
    山奈酚Kaempferol 19.051±0.150 b - 0.932
    AChE 苦参碱Matrine 1.095±0.146 b 0.913~1.327 0.974
    氧化苦参碱Oxymatrine 1.002±0.148 a 0.826~1.217 0.960
    山奈酚Kaempferol 3.683±0.154 c 2.536~6.714 0.790
    CarE 苦参碱Matrine 0.664±0.148 a 0.446~0.872 0.967
    氧化苦参碱Oxymatrine 1.283±0.141 b 1.063~1.600 0.947
    山奈酚Kaempferol 1.311±0.139 c 1.068~1.681 0.966
    注:IC50为抑制50%酶活性所需要的药剂质量浓度。不同小写字母表示不同药剂对同种酶的IC50之间差异显著(P<0.05)。Notes: IC50 values represent concentration for 50% inhibition of detoxifying enzymes. Different lowercase letters mean there is significant difference among IC50 values of varied treatments of plant secondary substances (P<0.05).
    下载: 导出CSV
  • [1] 陈澄宇, 康志娇, 史雪岩, 等.昆虫对植物次生物质的代谢适应机制及其对昆虫抗药性的意义[J].昆虫学报, 2015, 58(10):1126-1139. http://d.old.wanfangdata.com.cn/Periodical/kcxb201510011

    CHEN C Y, KANG Z J, SHI X Y, et al. Metabolic adaptation mechanisms of insects to plant secondary metabolites and their implications for insecticide resistance of insects[J]. Acta Entomologica Sinica, 2015, 58(10):1126-1139. http://d.old.wanfangdata.com.cn/Periodical/kcxb201510011
    [2] 李明, 曾任森, 骆世明.次生代谢产物在植物抵抗病虫为害中的作用[J].中国生物防治, 2007, 23(3):269-273. http://d.old.wanfangdata.com.cn/Periodical/zgswfz200703015

    LI M, ZENG R S, LUO S M. Secondary metabolites related with plant resistance against pathogenic microorganisms and insect pests[J]. Chinese Journal of Biological Control, 2007, 23(3):269-273. http://d.old.wanfangdata.com.cn/Periodical/zgswfz200703015
    [3] 孔垂华.21世纪植物化学生态学前沿领域[J].应用生态学报, 2002, 13(3):349-353. doi: 10.3321/j.issn:1001-9332.2002.03.023

    KONG C H. Frontier fields of plant chemical ecology in the 21st century[J]. The Journal of Applied Ecology, 2002, 13(3):349-353. doi: 10.3321/j.issn:1001-9332.2002.03.023
    [4] 陈晓亚, 刘培.植物次生代谢物的分子生物学及基因工程[J].生命科学, 1996, 8(2):8-11. http://www.cnki.com.cn/Article/CJFDTotal-SMKX602.003.htm

    CHEN X Y, LIU P. Molecular biology and genetic engineering of plant secondary metabolites[J]. Chinese Bulletin of Life Sciences, 1996, 8(2):8-11. http://www.cnki.com.cn/Article/CJFDTotal-SMKX602.003.htm
    [5] 杜近义, 胡国赋, 秦际威.植物次生代谢产物的生态学意义[J].生物学杂志, 1999, 16(5):9-10. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199900621496

    DU J Y, HU G F, QIN J W. The ecological significance of plant secondary metabolites[J]. Journal of Biology, 1999, 16(5):9-10. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199900621496
    [6] 高微微, 伶建明, 郭顺星.植物次生代谢产物的生态学功能研究进展[J].中国药学杂志, 2006, 41(13): 961-964. doi: 10.3321/j.issn:1001-2494.2006.13.001

    GAO W W, LING J M, GUO S X.Advances in ecological functions of plant secondary metabolites[J]. Chinese Pharmaceutical Journal, 2006, 41(13): 961-964. doi: 10.3321/j.issn:1001-2494.2006.13.001
    [7] 胡会平, 刘建慧, 孙鑫, 等.苦参碱及衍生物的生物活性和合成研究进展[J].鲁东大学学报(自然科学版), 2015, 31(4):339-345. doi: 10.3969/j.issn.1673-8020.2015.04.010

    HU H P, LIU J H, SUN X, et al. Research progress in biological activity and synthesis on matrine and its derivatives[J]. Journal of Ludong University (Natural Science Edition), 2015, 31(4):339-345. doi: 10.3969/j.issn.1673-8020.2015.04.010
    [8] 杨雪云, 赵博光, 巨云为.苦参碱和氧化苦参碱的抑菌活性及增效作用[J].南京林业大学学报(自然科学版), 2008, 32(2):79-82. doi: 10.3969/j.issn.1000-2006.2008.02.018

    YANG X Y, ZHAO B G, JU Y W. Antifungal activities and synergetic tests of matrine and oxymatrine to some tree pathogens[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2008, 32(2):79-82. doi: 10.3969/j.issn.1000-2006.2008.02.018
    [9] 赵妍.山奈酚对脂多糖诱导小鼠急性肺损伤的保护作用[D].哈尔滨: 东北农业大学, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10224-1013207331.htm

    ZHAO Y.Protective effect of kaempferol on acute lung injury induced by lipopolysaccharide in mice[D]. Harbin: Northeast Agricultural University, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10224-1013207331.htm
    [10] ZHANG J F, SUN J Z, WU Z B, et al. Identification of cotton varieties resistant to cannme spider mite and exploration of resistance mechanism[J]. Acta Phytophylacica Sinica, 1993, 20(2):155-161. http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZWBF199302012.htm
    [11] 彭露, 严盈, 刘万学, 等.植食性昆虫对植物的反防御机制[J].昆虫学报, 2010, 53(5):572-580. http://d.old.wanfangdata.com.cn/Periodical/kcxb201005012

    PENG L, YAN Y, LIU W X, et al. Counter-defense mechanisms of phytophagous insects towards plant defense[J]. Acta Entomologica Sinica, 2010, 53(5):572-580. http://d.old.wanfangdata.com.cn/Periodical/kcxb201005012
    [12] 姜礅, 孟昭军, 严善春.用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响[J].北京林业大学学报, 2017, 39(2):58-63. doi: 10.13332/j.1000-1522.20160291

    JIANG D, MENG Z J, YAN S C.Effects of partially spraying Larix olgensis seedlings with exogenous methyl jasmonate on the defensive enzyme activities of Dendrolimus superans larvae[J]. Journal of Beijing Forestry University, 2017, 39(2):58-63. doi: 10.13332/j.1000-1522.20160291
    [13] FELTON G W, TUMLINSON J H. Plant-insect dialogs: complex interactions at the plant-insect interface[J]. Current Opinion in Plant Biology, 2008, 11(4):457-463. doi: 10.1016/j.pbi.2008.07.001
    [14] YANG S, WU H, XIE J, et al. Depressed performance and detoxification enzyme activities of Helicoverpa armigera fed with conventional cotton foliage subjected to methyl jasmonate exposure[J]. Entomologia Experimentalis et Applicata, 2013, 147(2):186-195. doi: 10.1111/eea.12060
    [15] 张鑫乾, 严俊鑫, 杨杰莹, 等.重瓣玫瑰诱导抗性对双斑萤叶甲成虫解毒酶的影响[J].东北林业大学学报, 2014, 42(5):125-128. doi: 10.3969/j.issn.1000-5382.2014.05.030

    ZHANG X Q, YAN J X, YANG J Y, et al. Effect of induced resistance of Rosa rugosa 'plena' on the activities of detoxifying enzymes in Monolepta hieroglyhica (Mostschulsky)[J]. Journal of Northeast Forestry University, 2014, 42(5):125-128. doi: 10.3969/j.issn.1000-5382.2014.05.030
    [16] 李菁, 骆有庆, 石娟.利用植物源引诱剂监测与控制舞毒蛾[J].北京林业大学学报, 2011, 33(4):85-90. http://j.bjfu.edu.cn/article/id/9620

    LI J, LUO Y Q, SHI J.Use of phyto-attractant in monitoring and controlling gypsy moth[J]. Journal of Beijing Forestry University, 2011, 33(4):85-90. http://j.bjfu.edu.cn/article/id/9620
    [17] 胡春祥.舞毒蛾生物防治研究进展[J].东北林业大学学报, 2002, 30(4):40-43. doi: 10.3969/j.issn.1000-5382.2002.04.011

    HU C X. Research progress of the biological control for Lymantria dispar L.[J]. Journal of Northeast Forestry University, 2002, 30(4):40-43. doi: 10.3969/j.issn.1000-5382.2002.04.011
    [18] LAZAREVIC J, PERIC-MATARUGA V, IVANOVIC J, et al. Host plant effects on the genetic variation and correlations in the individual performance of the gypsy moth[J]. Functional Ecology, 1998, 12(1):141-148. doi: 10.1046/j.1365-2435.1998.00166.x
    [19] 丁吉同, 唐桦, 阿地力·沙塔尔, 等.4种植物源杀虫剂对亚洲型舞毒蛾幼虫的毒性与拒食作用[J].南京林业大学学报(自然科学版), 2013, 37(4):80-84. http://d.old.wanfangdata.com.cn/Periodical/njlydxxb201304015

    DING J T, TANG H, ADILI·SHATAER, et al. Comparison of the toxicities and antifeedant effects of four botanical insecticides against the larvae of Lymantria dispar asiatica Vnukovskij[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2013, 37(4):80-84. http://d.old.wanfangdata.com.cn/Periodical/njlydxxb201304015
    [20] 张凯, 周艳涛, 王皙玮, 等.铜、镉胁迫对舞毒蛾排毒代谢酶的影响[J].北京林业大学学报, 2016, 38(2):61-67. doi: 10.13332/j.1000-1522.20150293

    ZHANG K, ZHOU Y T, WANG X W, et al. Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar[J]. Journal of Beijing Forestry University, 2016, 38(2):61-67. doi: 10.13332/j.1000-1522.20150293
    [21] 邹传山, 曹传旺, 王志英.落叶松毛虫AChE最佳反应体系建立及其对3种杀虫剂敏感性比较[J].北京林业大学学报, 2013, 35(3):102-107. http://j.bjfu.edu.cn/article/id/9928

    ZOU C S, CAO C W, WANG Z Y. Determination of optimized reaction system for AChE in Dendrolimus superans and comparison of its sensitivity to three pesticides[J]. Journal of Beijing Forestry University, 2013, 35(3):102-107. http://j.bjfu.edu.cn/article/id/9928
    [22] VAN ASPEREN K. A study of housefly esterases by means of a sensitive colorimetric method[J]. Journal of Insect Physiology, 1962, 8(4):401-416. doi: 10.1016/0022-1910(62)90074-4
    [23] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1-2):248-254. doi: 10.1016/0003-2697(76)90527-3
    [24] 贾明慧, 张辉, 张爱华, 等.植物次生代谢产物在国内农药开发方面的研究进展[J].中国植保导刊, 2012, 32(3):15-18. doi: 10.3969/j.issn.1672-6820.2012.03.003

    JIA M H, ZHANG H, ZHANG A H, et al. Research progress on plant secondary metabolites in field of pesticide development in China[J]. China Plant Protection, 2012, 32(3):15-18. doi: 10.3969/j.issn.1672-6820.2012.03.003
    [25] PATCHARAPORN V. Insecticidal activity of five chinese medicinal plants against Plutella Xylostella L. (Lepidoptera: Plutellidae)[D]. Chongqing: Southwest University, 2010. https://www.sciencedirect.com/science/article/abs/pii/S1226861509001174
    [26] ISMAN M B, WAN A J, PASSREITER C M. Insecticidal activity of essential oils to the tobacco cutworm, Spodoptera litura[J]. Fitoterapia, 2001, 72(1):65-68. doi: 10.1016/S0367-326X(00)00253-7
    [27] 黄继光, 杨文杰, 桑晓清, 等.从羽裂蟹甲草分离的9种化合物的杀虫活性[J].华南农业大学学报, 2014, 35(1):64-68. http://d.old.wanfangdata.com.cn/Periodical/hnnydxxb201401012

    HUANG J G, YANG W J, SANG X Q, et al. Insecticidal activities of nine compounds extracted from Cacalia tangutica[J]. Journal of South China Agricultural University, 2014, 35(1):64-68. http://d.old.wanfangdata.com.cn/Periodical/hnnydxxb201401012
    [28] GADE S, RAJAMANIKYAM M, VADLAPUDI V, et al. Acetylcholinesterase inhibitory activity of stigmasterol & hexacosanol is responsible for larvicidal and repellent properties of Chromolaena odorata[J]. Biochimica et Biophysica Acta-General Subjects, 2017, 1861(3):541-550. doi: 10.1016/j.bbagen.2016.11.044
    [29] TONG F, GROSS A D, DOLAN M C, et al. The phenolic monoterpenoid carvacrol inhibits the binding of nicotine to the housefly nicotinic acetylcholine receptor[J]. Pest Management Science, 2013, 69(7):775-780. doi: 10.1002/ps.3443
    [30] 黄露.苦参碱对家蚕的毒害作用研究[D].泰安: 山东农业大学, 2014. http://cdmd.cnki.com.cn/Article/CDMD-10434-1014341692.htm

    HUANG L. Study on matrine toxicity to Bombyx mori L.[D]. Taian: Shandong Agricultural University, 2014. http://cdmd.cnki.com.cn/Article/CDMD-10434-1014341692.htm
    [31] 穆荣娟.7种苦豆子生物碱对豆蚜Aphis Craccinora Koch几种酶系的影响[D].杨凌: 西北农林科技大学, 2014. http://cdmd.cnki.com.cn/Article/CDMD-10712-1014430152.htm

    MU R J. Effect of seven alkaloids from Sophora alopecuroids on several enzymes of Aphis craccinora Koch[D]. Yangling: Northwest A&F University, 2014. http://cdmd.cnki.com.cn/Article/CDMD-10712-1014430152.htm
    [32] 罗万春, 慕立义, 李云寿.植物源生物碱的杀虫作用[J].农药, 1997, 11(7):8-12. http://d.old.wanfangdata.com.cn/Conference/352583

    LUO W C, MU L Y, LI Y S. Insecticidal effects of plant alkaloids[J]. Pesticides, 1997, 11(7):8-12. http://d.old.wanfangdata.com.cn/Conference/352583
    [33] 李春梅, 郁建平.血根碱对菜青虫的生物活性[J].安徽农业科学, 2012, 40(31):15245-15246. doi: 10.3969/j.issn.0517-6611.2012.31.057

    LI C M, YU J P. Bioactivity of sanguinarine against Pieris rapae L.[J]. Journal of Anhui Agricultural Sciences, 2012, 40(31):15245-15246. doi: 10.3969/j.issn.0517-6611.2012.31.057
  • 加载中
图(4) / 表(2)
计量
  • 文章访问数:  1681
  • HTML全文浏览量:  233
  • PDF下载量:  42
  • 被引次数: 0
出版历程
  • 收稿日期:  2017-06-16
  • 修回日期:  2017-07-14
  • 刊出日期:  2017-11-01

目录

    /

    返回文章
    返回