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复配芽孢杆菌对撑绿杂交竹枯萎病的生物防治效果研究

李凤 刘会涛 田莎 刘应高

李凤, 刘会涛, 田莎, 刘应高. 复配芽孢杆菌对撑绿杂交竹枯萎病的生物防治效果研究[J]. 北京林业大学学报, 2018, 40(12): 76-84. doi: 10.13332/j.1000-1522.20170320
引用本文: 李凤, 刘会涛, 田莎, 刘应高. 复配芽孢杆菌对撑绿杂交竹枯萎病的生物防治效果研究[J]. 北京林业大学学报, 2018, 40(12): 76-84. doi: 10.13332/j.1000-1522.20170320
Li Feng, Liu Huitao, Tian Sha, Liu Yinggao. Biological control effect of compounded bacillus on wilt disease of Bambusa pervariabilis × Dendrocalamopsis daii[J]. Journal of Beijing Forestry University, 2018, 40(12): 76-84. doi: 10.13332/j.1000-1522.20170320
Citation: Li Feng, Liu Huitao, Tian Sha, Liu Yinggao. Biological control effect of compounded bacillus on wilt disease of Bambusa pervariabilis × Dendrocalamopsis daii[J]. Journal of Beijing Forestry University, 2018, 40(12): 76-84. doi: 10.13332/j.1000-1522.20170320

复配芽孢杆菌对撑绿杂交竹枯萎病的生物防治效果研究

doi: 10.13332/j.1000-1522.20170320
基金项目: 

四川省教育厅重点项目 09ZA068

详细信息
    作者简介:

    李凤。主要研究方向:森林病虫害防治研究。Email:li_feng2016@126.com  地址:611130四川省成都市温江区惠民路211号四川农业大学林学院

    责任作者:

    刘应高,教授,博士生导师。主要研究方向:森林病虫害防治研究。Email: 499130793@qq.com  地址:同上

  • 中图分类号: S763.1

Biological control effect of compounded bacillus on wilt disease of Bambusa pervariabilis × Dendrocalamopsis daii

  • 摘要: 目的通过对比单一芽孢杆菌和复配芽孢杆菌对撑绿杂交竹枯萎病的防治效果,为开发防治撑绿杂交竹枯萎病的稳定高效、低成本的复合菌剂提供理论基础。方法本文制备了解淀粉芽孢杆菌B01-2、枯草芽孢杆菌B23-1以及二者的复配菌株的发酵滤液,并采用菌丝生长速率法、孢子萌发法和盆栽试验法,研究了各菌株发酵产物对病原菌尖孢镰刀菌菌丝生长、孢子萌发以及对杂交竹抗性生理指标的影响。结果复配芽孢杆菌处理对病原菌抑制效果显著高于单一菌株。复配芽孢杆菌菌落直径和孢子萌发率分别为1.9 cm和8.8%;单一菌株B01-2和B23-1的菌落直径与孢子萌发率分别为3.4 cm、4.1 cm和17.8%、31.0%;盆栽防效试验中,芽孢杆菌处理组的枯萎病发病率比对照低。复配芽孢杆菌的发病率(50%)较单一菌株B01-2(60%)与B23-1(60%)更低;复配芽孢杆菌对撑绿杂交竹枯萎病的防治效果达到81.5%,高于单一菌株B01-2(70.4%)与B23-1(63.0%)。杂交竹叶片生理指标测定表明,3种处理的发酵液都能诱导激发杂交竹体内的过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)防御酶的活性,降低杂交竹叶片叶绿素的分解和减少丙二醛(MDA)的生成,触发植物的系统抗病性,增强植株免疫能力。此外,复配菌株较单一菌株对杂交竹叶片的生理指标的影响更明显。结论复配芽孢杆菌具有强大的拮抗活性,在室内盆栽苗条件下能够有效防治撑绿杂交竹枯萎病,具有潜在的应用开发前景。

     

  • 图  1  菌株B01-2和B23-1在同一平板上的生长情况

    A. B01-2;B. B01-2+B23-1(箭头方向为菌株B23-1划线培养方向)(the arrow is the direction of B23-1);C. B23-1

    Figure  1.  Co-culture condition of B01-2 and B23-1 strains

    图  2  芽孢杆菌发酵产物对尖孢镰刀菌病原菌菌丝的影响

    A.正常的病原菌菌丝Normal fungal pathogen hyphae;B. B23-1处理的病原菌菌丝Fungal pathogen hyphae treated by B23-1;C. B01-2处理的病原菌菌丝Fungal pathogen hyphae treated by B01-2;D.复配芽孢杆菌处理病原菌菌丝(10×100)Fungal pathogen hyphae treated by compounded bacillus

    Figure  2.  Effects of compounded bacillus on the fungal pathogen hyphae of Fusarium oxysporum

    图  3  芽孢杆菌对杂交竹叶片中SPAD值的影响

    Figure  3.  Effects of bacillus on SPAD value of bamboo leaf (Bambusa pervariabilis × Dendrocalamopsis daii)

    图  4  芽孢杆菌对撑绿杂交竹叶片中多酚氧化酶(PPO)活性的影响

    Figure  4.  Effects of bacillus on the PPO activity of Bambusa pervariabilis × Dendrocalamopsis daii

    图  5  芽孢杆菌对撑绿杂交竹叶片中过氧化物酶(POD)活性的影响

    Figure  5.  Effects of bacillus on the POD activity of Bambusa pervariabilis × Dendrocalamopsis daii

    图  6  芽孢杆菌对撑绿杂交竹叶片中苯丙氨酸解氨酶(PAL)的影响

    Figure  6.  Effects of bacillus on the PAL activity of Bambusa pervariabilis × Dendrocalamopsis daii

    图  7  芽孢杆菌对撑绿杂交竹叶片中丙二醛(MDA)含量的影响

    Figure  7.  Effects of bacillus on the MDA content of Bambusa pervariabilis × Dendrocalamopsis daii

    表  1  芽孢杆菌菌株对尖孢镰刀菌病原菌拮抗效果

    Table  1.   Antagonistic effect of bacillus strains on fungal pathogen of Fusarium oxysporum

    处理Treatment 菌落直径Colony diameter/cm 抑菌率Bacteriostasis rate/% 孢子萌发率Spore germination rate/%
    空白Control 6.8±0.2D 98.8±0.8D
    B01-2 3.4±0.3B 54.4±0.2B 17.8±1.9B
    B23-1 4.1±0.4C 42.7±0.4A 31.0±1.6C
    CS 1.9±0.2A 78.3±0.3C 8.8±0.8A
    注:同列不同大写字母表示在0.05水平差异显著。CS.复配菌株。下同。Notes: different capital letters in the same row mean significantly different at P < 0.05 level. CS, compounded strain. The same below.
    下载: 导出CSV

    表  2  芽孢杆菌对撑绿杂交竹枯萎病的盆栽防治效果

    Table  2.   Biological control effect of bacillus on wilt disease of Bambusa pervariabilis × Dendrocalamopsis daii

    处理Treatment 发病率Disease incidence rate/% 病情指数Disease index 防治效果Control effect/%
    a 0 0 0
    b 100 67.5±4.5C
    c 60 20.4±1.7B 70.4±4.1A
    d 60 24.6±1.1B 63.0±3.8A
    e 50 12.5±0.9A 81.5±5.5B
    注:各组有10次重复。a为清水(CK);b为枯萎病菌;c为枯萎病菌+ B01-2;d为枯萎病菌+B23-1;e为枯萎病菌+ B01-2+B23-1。下同。Notes: every treatment has ten replicates. Treatment a, b, c, d and e represent the group treated by water, Fusarium oxysporum, Fusarium oxysporum + B01-2, Fusarium oxysporum + B23-1, and Fusarium oxysporum + B01-2 + B23-1, respectively. The same below.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2017-09-05
  • 修回日期:  2018-05-16
  • 刊出日期:  2018-12-01

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