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白杨派无性系苗期耐盐性综合评价及筛选

杨传宝 孙超 李善文 姚俊修 刘敬国 矫兴杰

杨传宝, 孙超, 李善文, 姚俊修, 刘敬国, 矫兴杰. 白杨派无性系苗期耐盐性综合评价及筛选[J]. 北京林业大学学报, 2017, 39(10): 24-32. doi: 10.13332/j.1000-1522.20170323
引用本文: 杨传宝, 孙超, 李善文, 姚俊修, 刘敬国, 矫兴杰. 白杨派无性系苗期耐盐性综合评价及筛选[J]. 北京林业大学学报, 2017, 39(10): 24-32. doi: 10.13332/j.1000-1522.20170323
YANG Chuan-bao, SUN Chao, LI Shan-wen, YAO Jun-xiu, LIU Jing-guo, JIAO Xing-jie. Comprehensive evaluation and screening of salt tolerance for Leuce clones at nursery stage[J]. Journal of Beijing Forestry University, 2017, 39(10): 24-32. doi: 10.13332/j.1000-1522.20170323
Citation: YANG Chuan-bao, SUN Chao, LI Shan-wen, YAO Jun-xiu, LIU Jing-guo, JIAO Xing-jie. Comprehensive evaluation and screening of salt tolerance for Leuce clones at nursery stage[J]. Journal of Beijing Forestry University, 2017, 39(10): 24-32. doi: 10.13332/j.1000-1522.20170323

白杨派无性系苗期耐盐性综合评价及筛选

doi: 10.13332/j.1000-1522.20170323
基金项目: 

山东省农业良种工程项目 2012213

山东省重点研发计划项目 2017GNC11115

详细信息
    作者简介:

    杨传宝,博士生。主要研究方向:森林培育。Email:chuanbaoy@163.com  地址:310012 浙江省杭州市西湖区文一路310号国家林业局竹子研究开发中心

    责任作者:

    李善文,博士,研究员。主要研究方向:林木遗传育种。Email: lishanwen66@163.com   地址:250014 山东省济南市文化东路42号山东省林业科学研究院

  • 中图分类号: S722.3+3; S792.117

Comprehensive evaluation and screening of salt tolerance for Leuce clones at nursery stage

  • 摘要: 以10个白杨派无性系为试材进行盆栽试验,通过对比各无性系的盆栽苗在不同质量分数(0、0.1%、0.3%、0.5%和0.7%)NaCl单盐胁迫条件下生长和生理代谢等参数的变化,对白杨派无性系进行了耐盐性能分析与评价。结果表明:高盐胁迫明显抑制了10个无性系苗高和地径的增长率(P < 0.05),无性系0057表现出较强的速生性和高存活率;低盐胁迫对无性系生长和细胞膜系统的影响不明显,但能诱导细胞内抗氧化酶(SOD和POD)活性和可溶性蛋白含量的上升;随着土壤盐浓度的增加以及盐处理时间的延长,保护酶活性、可溶性蛋白含量、细胞膜透性和MDA含量与对照相比均有显著提高。无性系的SOD与POD、可溶性蛋白之间的耐盐系数呈显著正相关关系,POD与MDA之间的耐盐系数呈显著负相关,表明无性系可以通过积累大量可溶性蛋白,增强抗氧化酶活性减少MDA的积累,提高自身耐盐能力。利用主成分分析和隶属函数法对10个无性系的耐盐性进行综合评价,初步认为无性系0057、422和XJY具有显著耐盐性和速生性, 可作为盐碱地造林或抗逆育种的重要试验材料应用。

     

  • 图  1  NaCl胁迫对苗高增长率(A、B)和地径增长率(C、D)的影响

    Figure  1.  Effects of NaCl stress on height growth rate (A, B) and basal diameter growth rate (C, D)

    图  2  NaCl胁迫对细胞膜透性(A、B)、SOD活性(C、D)和POD活性(E、F)的影响

    Figure  2.  Effects of NaCl stress on membrane permeability (A, B), SOD activity (C, D) and POD activity (E, F)

    图  3  NaCl胁迫对MDA含量(A、B)和可溶性蛋白含量(C、D)的影响

    Figure  3.  Effects of NaCl stress on MDA content (A, B) and soluble protein content (C, D)

    表  1  试验材料

    Table  1.   Experimental materials

    无性系
    Clone
    简称
    Abbreviation
    毛白杨鲁毛50 Poplus tomentosa ‘LM50’ LM50
    毛白杨0057号P. tomentosa ‘0057’ 0057
    毛白杨1352号P. tomentosa ‘1352’ 1352
    毛白杨1418号P. tomentosa ‘1418’ 1418
    毛白杨0085号P. tomentosa ‘0085’ 0085
    毛白杨1008号P. tomentosa ‘1008’ 1008
    毛白杨3-5-3号P. tomentosa ‘3-5-3’ 3-5-3
    毛新杨×截叶毛白杨11号(P.tomentosa×P.bolleanaP.tomentosa ‘11’ 11
    新疆杨P.bolleana XJY
    毛新杨×银灰杨422号(P. tomentosa×P. bolleanaP. canescens ‘422’ 422
    下载: 导出CSV

    表  2  盐处理后10个白杨派无性系死亡率

    Table  2.   Mortality of 10 poplar clones in high concentration salt treatment

    %
    时间
    Time
    NaCl质量分数
    NaCl mass fraction
    LM50 0057 1352 1418 0085 1008 3-5-3 11 XJY 422
    第21天
    The 21st day
    0 0 0 0 0 0 0 0 0 0 0
    0.1% 0 0 0 0 0 0 0 0 0 0
    0.3% 0 0 0 0 0 0 0 0 0 0
    0.5% 0 0 0 0 0 0 0 0 0 0
    0.7% 0 0 0 100 0 100 0 100 0 0
    第28天
    The 28th day
    0 0 0 0 0 0 0 0 0 0 0
    0.1% 0 0 0 0 0 0 0 0 0 0
    0.3% 0 0 0 0 0 0 0 0 0 0
    0.5% 0 0 0 100 0 100 0 100 0 0
    0.7% 100 0 100 100 100 100 100 100 0 0
    下载: 导出CSV

    表  3  各项指标之间的皮尔逊相关分析

    Table  3.   Pearson correlation analysis of various indicators

    指标Index X1 X2 X3 X4 X5 X6
    苗高增长率Seedling height growth rate (X1) 1
    地径增长率Basal diameter growth rate (X2) 0.770**
    细胞膜透性Membrane permeability (X3) -0.790** -0.674*
    SOD活性SOD activity (X4) 0.421 0.123 -0.380
    可溶性蛋白含量Soluble protein content (X5) 0.457 0.341 -0.620 0.713*
    POD活性POD activity (X6) 0.591 0.398 -0.258 0.767** 0.528
    MDA含量MDA content (X7) -0.521 -0.556 0.356 -0.539 -0.257 -0.652*
    注:*表示相关显著(P < 0.05),**表示相关极显著(P<0.01)。Notes: * means significant correlation(P < 0.05), ** means very significant correlation (P < 0.01).
    下载: 导出CSV

    表  4  旋转后的主成分因子载荷矩阵及指标权重

    Table  4.   Factor loading matrix after rotation and index weights

    主成分
    Principal component
    X1 X2 X3 X4 X5 X6 X7 特征值
    Eigenvalue
    贡献率
    Contribution rate/%
    1 0.862 0.727 -0.768 0.734 0.730 0.786 -0.723 4.074 58.204
    2 -0.349 -0.580 0.430 0.624 0.255 0.433 -0.094 1.294 18.479
    3 -0.027 -0.190 -0.384 0.121 0.560 -0.276 0.574 0.919 13.122
    综合得分系数
    Comprehensive score coefficient (Vj)
    0.055 0.026 -0.060 0.097 0.097 0.076 -0.043
    权重Index weight (Wj) 0.122 0.058 0.132 0.214 0.213 0.168 0.094
    下载: 导出CSV

    表  5  耐盐性综合评价

    Table  5.   Comprehensive assessment of salt tolerance of ten clones

    指标 Index LM50 0057 1352 1418 0085 1008 3-5-3 11 XJY 422
    X1 0.477 0.498 0.412 0.467 0.514 0.396 0.371 0.476 0.375 0.453
    X2 0.493 0.465 0.432 0.445 0.367 0.482 0.382 0.331 0.522 0.471
    X3 0.516 0.623 0.524 0.407 0.422 0.384 0.512 0.407 0.559 0.587
    X4 0.431 0.588 0.457 0.394 0.415 0.544 0.486 0.548 0.599 0.588
    X5 0.552 0.596 0.511 0.494 0.476 0.462 0.508 0.487 0.586 0.578
    X6 0.533 0.572 0.481 0.402 0.433 0.378 0.459 0.355 0.506 0.481
    X7 0.579 0.613 0.521 0.466 0.544 0.485 0.544 0.409 0.558 0.593
    D 0.509 0.576 0.481 0.437 0.454 0.451 0.475 0.450 0.540 0.546
    排序Order 4 1 5 10 7 8 6 9 3 2
    下载: 导出CSV
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  • 收稿日期:  2017-09-08
  • 修回日期:  2017-09-28
  • 刊出日期:  2017-10-01

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