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旱柳野生种质资源收集及多样性分析

于振旭 秦光华 宋玉民 乔玉玲 姜岳忠 王华田

于振旭, 秦光华, 宋玉民, 乔玉玲, 姜岳忠, 王华田. 旱柳野生种质资源收集及多样性分析[J]. 北京林业大学学报, 2018, 40(10): 67-76. doi: 10.13332/j.1000-1522.20170330
引用本文: 于振旭, 秦光华, 宋玉民, 乔玉玲, 姜岳忠, 王华田. 旱柳野生种质资源收集及多样性分析[J]. 北京林业大学学报, 2018, 40(10): 67-76. doi: 10.13332/j.1000-1522.20170330
Yu Zhenxu, Qin Guanghua, Song Yumin, Qiao Yuling, Jiang Yuezhong, Wang Huatian. Collection and genetic diversity analysis of wild germplasm in Salix matsudana[J]. Journal of Beijing Forestry University, 2018, 40(10): 67-76. doi: 10.13332/j.1000-1522.20170330
Citation: Yu Zhenxu, Qin Guanghua, Song Yumin, Qiao Yuling, Jiang Yuezhong, Wang Huatian. Collection and genetic diversity analysis of wild germplasm in Salix matsudana[J]. Journal of Beijing Forestry University, 2018, 40(10): 67-76. doi: 10.13332/j.1000-1522.20170330

旱柳野生种质资源收集及多样性分析

doi: 10.13332/j.1000-1522.20170330
基金项目: 

林业公益性行业科研专项 201404107

详细信息
    作者简介:

    于振旭,博士。主要研究方向:森林培育。Email:taiyangxu2009@163.com 地址:250000 山东省济南市历下区文化东路42号

    责任作者:

    秦光华,研究员。主要研究方向:林木遗传育种。Email:guanghuaqin@163.com 地址:同上

  • 中图分类号: S792.12

Collection and genetic diversity analysis of wild germplasm in Salix matsudana

  • 摘要: 目的在山东境内调查收集旱柳优树种质,探索旱柳种内遗传多样性水平。方法在试验区建立旱柳无性系苗期对比试验林,利用2年生苗木,分别进行表型数据观测和分子水平遗传多样性标记,分析旱柳种内遗传多样性。结果旱柳种质资源群体内存在丰富的表型变异和分子遗传变异:不同无性系枝干颜色呈黄绿、灰绿、绿色、灰褐、红褐、褐色,且色差重叠少,差异显著;主干有直立、稍弯、中等弯曲、较强弯曲、扭曲,小枝有上弯、直立、下垂,且形态差异显著;不同无性系树高变异范围为1.2~5.5m,地径为0.6~4.6cm,叶长为8.5~18cm,叶宽为0.92~3.49cm,叶面积为6.258~33.584cm2,差异极显著(P < 0.001)。叶长和叶面积的极端差异来源主要是旱柳无性系内,树高、地径、叶宽的极端差异则主要来源于无性系间。表型变异主要来源于无性系间,无性系间的性状分化对旱柳种内性状分化的贡献率达80%以上,说明无性系间的性状分化是旱柳种内性状分化的主要原因。AFLP结果显示,旱柳观测位点的平均多态性比率高达99.5%,显示出极高的多态性;多样性指数结果显示,Nei’s遗传多样度(H)为0.2322,Shannon信息指数(I)为0.3746,表明55份种质在种水平上属于同一个种,与主成分分析及聚类结果一致。结论试验证明旱柳种内存在丰富的表型及分子遗传多样性,是优良的选择和杂交育种材料。

     

  • 图  1  旱柳种质资源收集图

    Figure  1.  Collection of S. matsudana germplasm resources

    图  2  旱柳种内质量性状对比图

    Figure  2.  Comparison of qualitative traits of S. matsudana

    图  3  旱柳无性系个体及群体苗高、地径

    Figure  3.  Seedling height and ground diameter of S. matsudana individuals and groups

    图  4  旱柳叶长、叶宽及叶面积分布图

    Figure  4.  Distribution of leaf length, leaf width and leaf area of S. matsudana

    图  5  旱柳无性系各性状相对极差及表型分化系数图

    Figure  5.  Relative range and phenotypic differentiation coefficient of each trait of S. matsudana clones

    图  6  旱柳55份种质DNA凝胶电泳(引物组合EAGG/MCTA)

    此图为部分试验结果展示,每个泳道代表一个样品,从左到右为样品S. matsudana 1到S. matsudana 30。

    Figure  6.  Gel electrophoretic results of 55 germplasm resources of S. matsudana germplasm (primer combination EAGG/MCTA)

    This picture shows part of the whole result, and each lane means one specimen; S. matsudana 1 to S. matsudana 30 is from left to right.

    图  7  旱柳无性系聚类分析

    Figure  7.  Cluster analysis of S. matsudana clones

    图  8  旱柳无性系主成分分析

    Figure  8.  Principal component analysis of S. matsudana clones

    表  1  旱柳种质资源名录

    Table  1.   List of S. matsudana germplasm resources

    编号Code 收集地
    Collection place
    纬度
    Latitude
    经度
    Longitude
    地貌
    Landform
    S. matsudana 8 滨城区
    Bincheng District
    37°27′12″N 118°05′55″E 平原Plain
    S. matsudana 24 37°27′12″N 118°05′55″E 平原Plain
    S. matsudana 34 37°27′06″N 118°06′07″E 平原Plain
    S. matsudana 16 博兴县Boxing County 37°21′32″N 118°09′11″E 河岸River bank
    S. matsudana 11 37°13′32″N 118°15′56″E 平原Plain
    S. matsudana 41 单县Shanxian County 34°36′36″N 116°00′13″E 平原Plain
    S. matsudana 12 河口区Hekou District 37°57′28″N 118°19′49″E 平原Plain
    S. matsudana 40 37°57′13″N 118°20′17″E 平原Plain
    S. matsudana 31 37°57′13″N 118°20′17″E 平原Plain
    S. matsudana 32 37°51′06″N 118°21′02″E 平原Plain
    S. matsudana 4 惠民县Huimin County 37°14′23″N 117°31′21″E 平原Plain
    S. matsudana 3 37°19′45″N 117°27′55″E 平原Plain
    S. matsudana 36 37°27′11″N 117°31′16″E 平原Plain
    S. matsudana 45 历城区Licheng District 36°43′16″N 117°08′50″E 平原Plain
    S. matsudana 25 36°26′24″N 117°07′33″E 平原Plain
    S. matsudana 50 36°26′34″N 117°07′05″E 平原Plain
    S. matsudana 43 垦利县Kenli County 37°32′57″N 118°19′30″E 平原Plain
    S. matsudana 9 利津县Lijin County 37°27′59″N 118°11′35″E 平原Plain
    S. matsudana 39 37°31′03″N 118°15′58″E 平原Plain
    S. matsudana 35 37°35′47″N 118°20′12″E 平原Plain
    S. matsudana 28 蒙阴县Mengyin County 35°36′42″N 117°59′52″E 平原Plain
    S. matsudana 19 商河县Shanghe County 37°20′56″N 117°10′44″E 平原Plain
    S. matsudana 30 37°20′50″N 117°11′08″E 平原Plain
    S. matsudana 55 37°22′37″N 117°10′34″E 平原Plain
    S. matsudana 42 37°25′46″N 117°13′26″E 平原Plain
    S. matsudana 46 37°26′02″N 117°13′33″E 平原Plain
    S. matsudana 37 37°20′23″N 117°14′49″E 平原Plain
    S. matsudana 22 37°20′16″N 117°15′46″E 平原Plain
    S. matsudana 21 泗水县Sishui County 35°39′46″N 117°15′57″E 平原Plain
    S. matsudana 18 35°39′46″N 117°15′57″E 河岸River bank
    S. matsudana 48 35°36′01″N 117°02′49″E 平原Plain
    S. matsudana 13 35°42′20″N 117°18′14″E 平原Plain
    S. matsudana 20 无棣县Wudi County 37°45′12″N 117°37′28″E 平原Plain
    S. matsudana 5 新泰市Xintai City 35°54′32″N 117°46′28″E 河岸River bank
    S. matsudana 33 35°54′30″N 117°46′27″E 河岸River bank
    S. matsudana 44 35°54′49″N 117°46′32″E 河岸River bank
    S. matsudana 10 35°56′40″N 117°51′09″E 平原Plain
    S. matsudana 23 兖州市Yanzhou City 35°42′05″N 116°41′02″E 平原Plain
    S. matsudana 1 禹城市Yucheng City 36°58′55″N 116°40′03″E 河岸River bank
    S. matsudana 6 37°03′35″N 116°41′01″E 平原Plain
    S. matsudana 47 37°10′49″N 116°42′50″E 平原Plain
    S. matsudana 53 37°01′56″N 116°37′05″E 平原Plain
    S. matsudana 27 沾化县Zhanhua County 37°40′24″N 118°05′48″E 平原Plain
    S. matsudana 15 37°39′08″N 118°04′50″E 平原Plain
    S. matsudana 14 37°42′04″N 118°08′08″E 平原Plain
    S. matsudana 51 周村区Zhoucun District 36°47′54″N 117°54′42″E 平原Plain
    S. matsudana 49 36°47′57″N 117°55′06″E 平原Plain
    S. matsudana 52 36°47′59″N 117°55′23″E 平原Plain
    S. matsudana 26 张店区Zhangdian District 36°48′10″N 117°58′00″E 河岸River bank
    S. matsudana 17 36°48′07″N 117°58′25″E 平原Plain
    S. matsudana 29 大汶流域Dawen River Basin 37°45′18″N 119°06′56″E 平原Plain
    S. matsudana 7 37°45′18″N 119°06′56″E 平原Plain
    S. matsudana 2 37°44′51″N 119°09′59″E 平原Plain
    S. matsudana 54 37°44′49″N 119°08′24″E 平原Plain
    S. matsudana 38 37°45′08″N 119°07′23″E 平原Plain
    下载: 导出CSV

    表  2  旱柳质量性状赋值参照表

    Table  2.   Given value of qualitative characters of S. matsudana

    性状Trait 项目Item 描述分级及赋值Discrimination classification and valuation
    主干干型
    Stem form
    特征
    Trait
    直立
    Up-right
    稍弯
    Slightly curved
    中等弯曲
    Medium curved
    较强弯曲
    Highly curved
    扭曲
    Strongly curved
    赋值Value 1 2 3 4 5
    枝干颜色
    Stem color
    特征
    Trait
    黄绿
    Yellowish-green
    灰绿
    Grayish-green
    绿
    Green
    灰褐
    Grayish-brown
    红褐
    Reddish-brown
    褐色
    Brown
    赋值Value 1.3 1.6 1.9 2.3 2.6 2.9
    小枝赋值
    Value of branchlet
    1.3 1.6 1.9 2.3 2.6 2.9
    枝条形态 特征Trait 上弯Upswept 直伸Straight 下垂Droop
    Branchlet form 赋值Value 1 2 3
    叶着生方式 特征Trait 对生Opposite 互生Mutually
    Leaf growing style 赋值Value 1 2
    叶形Leaf shape 特征Trait 线形Linear 披针形Lanceolate 卵形Oval
    赋值Value 1 2 3
    叶缘Leaf margin 特征
    Trait
    全缘
    Entire
    细锯齿
    Serrulation
    宽锯齿
    Broadly blade
    赋值Value 1 2.1 2.2
    叶表/背被毛情况Pilose on/under leaf 特征
    Trait
    无或很少
    None or rarely

    Little

    Medium

    Much
    很多
    Dense
    上表面赋值Value (up) 1 2 3 4 5
    下表面赋值Value (below) 1 2 3 4 5
    下载: 导出CSV

    表  3  利用7对引物对55份旱柳种质的DNA扩增结果

    Table  3.   DNA amplification results of 55 germplasm resources of S. matsudana germplasm by 7 primer combinations

    引物组合
    Primer combination
    总位点数
    Total number of loci
    多态性位点数
    Number of polymorphic loci
    多态率
    Polymorphism/%
    EAAG/MCAG 172 172 100
    EAGC/MCAA 150 150 100
    EAGC/MCAG 144 142 98.61
    EAGG/MCAA 146 145 99.32
    EAGG/MCAC 212 212 100
    EAGG/MCAG 164 163 99.3
    EAGG/MCTA 134 133 99.25
    总计Total 1122 1117 99.5
    平均Mean 160.29 159.57 99.5
    下载: 导出CSV
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  • 收稿日期:  2017-09-12
  • 修回日期:  2018-01-26
  • 刊出日期:  2018-10-01

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