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无髯鸢尾种间杂交障碍分析

李丛丛 高亦珂 刘蓉 曹颖 范诸平 郭翎 张启翔

李丛丛, 高亦珂, 刘蓉, 曹颖, 范诸平, 郭翎, 张启翔. 无髯鸢尾种间杂交障碍分析[J]. 北京林业大学学报, 2018, 40(4): 96-101. doi: 10.13332/j.1000-1522.20170351
引用本文: 李丛丛, 高亦珂, 刘蓉, 曹颖, 范诸平, 郭翎, 张启翔. 无髯鸢尾种间杂交障碍分析[J]. 北京林业大学学报, 2018, 40(4): 96-101. doi: 10.13332/j.1000-1522.20170351
Li Congcong, Gao Yike, Liu Rong, Cao Ying, Fan Zhuping, Guo Ling, Zhang Qixiang. Analysis on the barriers of interspecific hybridization in beardless irises[J]. Journal of Beijing Forestry University, 2018, 40(4): 96-101. doi: 10.13332/j.1000-1522.20170351
Citation: Li Congcong, Gao Yike, Liu Rong, Cao Ying, Fan Zhuping, Guo Ling, Zhang Qixiang. Analysis on the barriers of interspecific hybridization in beardless irises[J]. Journal of Beijing Forestry University, 2018, 40(4): 96-101. doi: 10.13332/j.1000-1522.20170351

无髯鸢尾种间杂交障碍分析

doi: 10.13332/j.1000-1522.20170351
基金项目: 

“863”国家高技术研究发展计划项目 2013AA102706

详细信息
    作者简介:

    李丛丛。主要研究方向:花卉种质创新与育种。Email: licongcong0616@126.com 地址:100083北京市海淀区清华东路35号北京林业大学园林学院

    责任作者:

    高亦珂,博士,教授。主要研究方向:花卉育种与花卉混播。Email: gaoyk@bjfu.edu.cn 地址:同上

  • 中图分类号: S682.1+9

Analysis on the barriers of interspecific hybridization in beardless irises

  • 摘要: 目的为探究无髯鸢尾种间杂交障碍原因及子房败育时间,以西伯利亚鸢尾5个品种为亲本,与黄菖蒲、喜盐鸢尾分别进行常规杂交。方法授粉后观测种间杂交后子房发育动态,并绘制发育动态折线图;在荧光显微镜下,观测不同授粉时间后的花粉管原位萌发动态,并统计每个杂交组合的杂交结实率和种子萌发率。结果无髯鸢尾种间杂交子房在授粉后9~16 d发生败育;授粉后杂交障碍原因主要是花粉附着困难、花粉萌发率低、花粉管伸长障碍和胚囊败育的受精后障碍;黄菖蒲与西伯利亚鸢尾杂交后受精前和受精后障碍并存,喜盐鸢尾与西伯利亚鸢尾杂交主要存在受精后障碍;喜盐鸢尾与西伯利亚鸢尾的亲和性高于其与黄菖蒲杂交的亲和性。结论花粉原位萌发可作为远缘杂交前障碍检测指标,观测花粉原位萌发可及时采用相应措施,增大杂种获得率;不同品种种间亲和性不同,二倍体和四倍体在染色体条数不同的种间远缘杂交中亲和性无显著区别;不同种间杂交,正反交杂交障碍类型不同,可以通过不同种系品种间正反交,增加无髯鸢尾种间杂交亲和性,获得变异丰富的种间杂交类型。

     

  • 图  1  子房生长趋势

    Figure  1.  Trend of ovary growing

    图  2  花粉管萌发

    A.I.pseudacorus בLichter feidills’授粉后2 h;B. I. pseudacorus בLichter feidills’授粉后6 h;C.I. pseudacorus בFourfold Lavendel’授粉后48 h;D. ‘Dancing Nanou’ × I. pseudacorus授粉后48 h;E. ‘Big’s child’ × I. pseudacorus授粉后48 h;F. ‘Fourfold Lavendel’ × I. pseudacorus授粉后48 h;G.‘Viel Creme’ ×I. pseudacorus授粉后48 h;H. ‘Lichter feidills’ × I.pseudacorus授粉后48 h;I. I. halophila var. sogdiana × ‘Lichter feidills’授粉后2 h;J. I. halophila var. sogdiana × ‘Viel Creme’授粉后2 h;K. I. halophila var. sogdiana × ‘Dancing Nanou’授粉后48 h;L. I. halophila var. sogdiana × ‘Lichter feidills’授粉后48 h;M. I. halophila var. sogdiana בFourfold Lavendel’授粉后48 h;N. ‘Dancing Nanou’ × I. halophila var. sogdiana 授粉后2 h;O. ‘Big’s child’ × I. halophila var.sogdiana授粉后48 h;P. ‘Lichter feidills’ × I. halophila var. sogdiana授粉后24 h。

    Figure  2.  Germination of pollen

    A, 2 hours after pollination for I. pseudacorus × 'Lichter feidills'; B, 6 hours after pollination for I. pseudacorus × 'Lichter feidills'; C, 48 hours after pollination for I. pseudacorus × 'Fourfold Lavendel'; D, 48 hours after pollination for 'Dancing Nanou' × I. pseudacorus; E, 48 hours after pollination for 'Big's child' × I. pseudacorus; F, 48 hours after pollination for 'Fourfold Lavendel' × I. pseudacorus; G, 48 hours after pollination for 'Viel Creme' × I. pseudacorus; H, 48 hours after pollination for 'Lichter feidills' × I. pseudacorus; I, 2 hours after pollination for I. halophila var. Sogdiana ×'Lichter feidills'; J, 2 hours after pollination for I. halophila var. sogdiana × 'Viel Creme'; K, 48 hours after pollination for I. halophila var. sogdiana × 'Dancing Nanou'; L, 48 hours after pollination for I. halophila var. sogdiana × 'Lichter feidills'; M, 48 hours after pollination for I. halophila var. sogdiana ×'Fourfold Lavendel'; N, 2 hours after pollination for 'Dancing Nanou' × I. halophila var. sogdiana; O, 48 hours after pollination for 'Big's child' × I. halophila var. sogdiana; P, 24 hours after pollination for 'Lichter feidills' × I. halophila var. sogdiana.

    表  1  杂交结实萌发情况

    Table  1.   Germination condition of hybrid seed setting

    杂交组合
    Cross combination
    杂交数量
    Pollination quantity
    结实率
    Fruit setting rate/%
    种子数量
    Seed quantity
    萌发率
    Germination rate/%
    I.pseudacorus × ‘Dancing Nanou’ 50 8.00 83 43.37
    I.pseudacorus × ‘Lichter feidills’ 53 3.77 5 0.00
    I.pseudacorus × ‘Big’s child’ 33 0.00 0 0.00
    I.pseudacorus × ‘Fourfold Lavendel’ 51 3.92 12 25.00
    I.pseudacorus × ‘Viel Creme’ 43 2.32 2 0.00
    ‘Dancing Nanou’ × I. pseudacorus 65 26.15 487 0.41
    ‘Lichter feidills’ × I. pseudacorus 137 0.00 0 0.00
    ‘Big’s child’ × I. pseudacorus 13 92.31 250 0.00
    ‘Fourfold Lavendel’ × I. pseudacorus 90 10.00 169 11.83
    ‘Viel Creme’ × I. pseudacorus 47 31.91 357 0.00
    I.halophila var . sogdiana× ‘Dancing Nanou’ 104 95.96 518 55.21
    I.halophila var . sogdiana× ‘Lichter feidills’ 34 26.47 34 14.71
    I.halophila var . sogdiana× ‘Big’s child’ 16 87.50 14 92.86
    I.halophila var . sogdiana× ‘Fourfold Lavendel’ 56 3.57 8 0.00
    I.halophila var . sogdiana× ‘Viel Creme’ 50 0.00 0 0.00
    ‘Dancing Nanou’ × I. halophila var. sogdiana 66 0.00 0 0.00
    ‘Lichter feidills’ × I. halophila var. sogdiana 116 0.00 0 0.00
    ‘Big’s child’ × I. halophila var. sogdiana 22 0.00 0 0.00
    ‘Fourfold Lavendel’ × I. halophila var. sogdiana 117 3.42 80 27.50
    ‘Viel Creme’ × I. halophila var. sogdiana 97 12.37 168 0.00
    下载: 导出CSV
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  • 收稿日期:  2017-09-26
  • 修回日期:  2018-01-08
  • 刊出日期:  2018-04-01

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