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NaOH-乙醇预处理提高甘蔗渣酶法制备低聚木糖效率

杨淑娟 游艳芝 张威伟 王堃 蒋建新

杨淑娟, 游艳芝, 张威伟, 王堃, 蒋建新. NaOH-乙醇预处理提高甘蔗渣酶法制备低聚木糖效率[J]. 北京林业大学学报, 2018, 40(2): 114-120. doi: 10.13332/j.1000-1522.20170366
引用本文: 杨淑娟, 游艳芝, 张威伟, 王堃, 蒋建新. NaOH-乙醇预处理提高甘蔗渣酶法制备低聚木糖效率[J]. 北京林业大学学报, 2018, 40(2): 114-120. doi: 10.13332/j.1000-1522.20170366
Yang Shu-juan, You Yan-zhi, Zhang Wei-wei, Wang Kun, Jiang Jian-xin. NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2018, 40(2): 114-120. doi: 10.13332/j.1000-1522.20170366
Citation: Yang Shu-juan, You Yan-zhi, Zhang Wei-wei, Wang Kun, Jiang Jian-xin. NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2018, 40(2): 114-120. doi: 10.13332/j.1000-1522.20170366

NaOH-乙醇预处理提高甘蔗渣酶法制备低聚木糖效率

doi: 10.13332/j.1000-1522.20170366
基金项目: 

国家重点研发计划课题 2016YFD0600803

详细信息
    作者简介:

    杨淑娟。主要研究方向:生物质能源及化学品。Email:yang2017bjfu@163.com 地址:100083北京市海淀区清华东路35号北京林业大学材料科学与技术学院

    责任作者:

    蒋建新,教授,博士生导师。主要研究方向:林产化工及生物质能源材料。Email: jiangjx@bjfu.edu.cn 地址:同上

  • 中图分类号: Q556

NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis

  • 摘要: 目的对甘蔗渣酶法制备低聚木糖(XOS)的工艺进行研究,并通过NaOH-乙醇预处理提高低聚木糖的生产效率。方法首先,对预处理前后甘蔗渣的化学组成进行表征,确定预处理对原料组分的影响。其次,利用接触角和X射线衍射分析技术,探讨预处理对底物湿部化学(润湿性)特性和物理结构的影响。最后,通过高效液相色谱(HPLC)分析检测木聚糖酶水解样品,比较不同预处理强度对酶水解生产低聚木糖质量浓度的影响。结果对于NaOH-乙醇预处理促进木聚糖酶水解的工艺而言,最佳的预处理条件为10 g/L NaOH-乙醇(乙醇的体积分数为50%)预处理。在该预处理强度下,大量的木质素被脱除,脱除率可以达到78.10%;而且,该预处理方式能够有效改善物料的亲水性能,使接触角从61.5°降低到55.4°,同时将纤维原料的结晶度从28.6%提高到32.3%。通过分析酶水解样品可知:当NaOH用量为10 g/L时,可以实现最高低聚木糖质量浓度(1.85 g/L),与未处理原料(0.83 g/L)相比,提高了122.89%。结论对于甘蔗渣制备低聚木糖的工艺而言,采用木聚糖酶水解的方式能够实现从半纤维素到低聚木糖的有效转化,并且采用NaOH-乙醇预处理可以有效提高甘蔗渣的酶解效率,促进低聚木糖的生产。

     

  • 图  1  低聚木聚糖标准品色谱图

    X1代表木糖;X2代表木二糖;X3代表木三糖;X4代表木四糖;X5代表木五糖。

    Figure  1.  Chromatogram of xylo-oligosaccharide (XOS) standard

    X1 represents xylose, X2 represents xylobiose, X3 represents xylotriose, X4 represents xylotetraose, and X5 represents xylopentaose.

    图  2  预处理前后甘蔗渣的接触角变化

    Figure  2.  Contact angles of untreated and pretreated sugarcane bagasse

    图  3  预处理前后甘蔗渣的X射线衍射谱图

    Figure  3.  X-ray diffraction spectra of untreated and pretreated sugarcane bagasse

    图  4  酶解时间对低聚木糖和木糖质量浓度的影响

    Figure  4.  Effects of time on the mass concentration of XOS and xylose

    图  5  预处理对低聚木糖初始生产速率的影响

    Figure  5.  Effects of pretreatment on the initial production rate of XOS

    图  6  预处理对低聚木糖生产的影响

    Figure  6.  Effects of pretreatments on XOS production

    表  1  NaOH-乙醇预处理甘蔗渣化学组成

    Table  1.   Chemical compositions of sugarcane bagasse pretreated by NaOH-ethanol

    NaOH-乙醇预处理
    NaOH-ethanol pretreatment
    葡聚糖质量分数
    Mass fraction of glucan
    木聚糖质量分数
    Mass fraction of xylan
    木质素质量分数
    Mass fraction of lignin
    灰分质量分数
    Mass fraction of ash
    固体得率
    Solid yield/%
    未处理Untreated (45.25±0.31)% (23.30±0.30)% (21.64±0.12)% (1.94±0.04)% 100.00
    5 g/L (52.68±0.33)% (26.31±0.24)% (16.30±0.08)% (1.20±0.06)% 84.30
    10 g/L (60.96±0.17)% (27.50±0.18)% (8.50±0.11)% (0.91±0.08)% 71.67
    15 g/L (60.84±0.15)% (28.62±0.09)% (6.69±0.31)% (1.09±0.03)% 70.85
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  • 收稿日期:  2017-10-12
  • 修回日期:  2017-11-30
  • 刊出日期:  2018-02-01

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