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    杨淑娟, 游艳芝, 张威伟, 王堃, 蒋建新. NaOH-乙醇预处理提高甘蔗渣酶法制备低聚木糖效率[J]. 北京林业大学学报, 2018, 40(2): 114-120. DOI: 10.13332/j.1000-1522.20170366
    引用本文: 杨淑娟, 游艳芝, 张威伟, 王堃, 蒋建新. NaOH-乙醇预处理提高甘蔗渣酶法制备低聚木糖效率[J]. 北京林业大学学报, 2018, 40(2): 114-120. DOI: 10.13332/j.1000-1522.20170366
    Yang Shu-juan, You Yan-zhi, Zhang Wei-wei, Wang Kun, Jiang Jian-xin. NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2018, 40(2): 114-120. DOI: 10.13332/j.1000-1522.20170366
    Citation: Yang Shu-juan, You Yan-zhi, Zhang Wei-wei, Wang Kun, Jiang Jian-xin. NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2018, 40(2): 114-120. DOI: 10.13332/j.1000-1522.20170366

    NaOH-乙醇预处理提高甘蔗渣酶法制备低聚木糖效率

    NaOH-ethanol pretreatment increasing preparation efficiency of xylo-oligosaccharide from sugarcane bagasse with enzymatic hydrolysis

    • 摘要:
      目的对甘蔗渣酶法制备低聚木糖(XOS)的工艺进行研究,并通过NaOH-乙醇预处理提高低聚木糖的生产效率。
      方法首先,对预处理前后甘蔗渣的化学组成进行表征,确定预处理对原料组分的影响。其次,利用接触角和X射线衍射分析技术,探讨预处理对底物湿部化学(润湿性)特性和物理结构的影响。最后,通过高效液相色谱(HPLC)分析检测木聚糖酶水解样品,比较不同预处理强度对酶水解生产低聚木糖质量浓度的影响。
      结果对于NaOH-乙醇预处理促进木聚糖酶水解的工艺而言,最佳的预处理条件为10 g/L NaOH-乙醇(乙醇的体积分数为50%)预处理。在该预处理强度下,大量的木质素被脱除,脱除率可以达到78.10%;而且,该预处理方式能够有效改善物料的亲水性能,使接触角从61.5°降低到55.4°,同时将纤维原料的结晶度从28.6%提高到32.3%。通过分析酶水解样品可知:当NaOH用量为10 g/L时,可以实现最高低聚木糖质量浓度(1.85 g/L),与未处理原料(0.83 g/L)相比,提高了122.89%。
      结论对于甘蔗渣制备低聚木糖的工艺而言,采用木聚糖酶水解的方式能够实现从半纤维素到低聚木糖的有效转化,并且采用NaOH-乙醇预处理可以有效提高甘蔗渣的酶解效率,促进低聚木糖的生产。

       

      Abstract:
      ObjectiveThe condition of preparation of xylo-oligosaccharide (XOS) from sugarcane bagasse with enzymatic hydrolysis was investigated. In this study, the preparation efficiency of xylo-oligosaccharide was improved by NaOH-ethanol pretreatment.
      MethodFirstly, the chemical composition of the sugarcane bagasse before and after pretreatment was characterized, and the effect of pretreatment on the components of the raw material was determined. Secondly, the wettability and physical structure of substrates were characterized by contact angle analysis and X-ray diffraction (XRD). Finally, in order to compare the effects of different pretreatments on the mass concentration of XOS with enzymatic hydrolysis, the enzymatic hydrolysate was harvested and analyzed by high performance liquid chromatography (HPLC).
      ResultCharacterization results confirmed that optimal pretreatment condition was 10 g/L NaOH-ethanol (50% v/v ethanol) pretreatment. Under this pretreatment condition, a large amount of lignin was removed and the removal rate of lignin could reach 78.10%. Moreover, the pretreatment could effectively improve the hydrophilicity of raw materials, decreased the contact angle from 61.5° to 55.4°, and also increased the crystallinity of lignocellulose from 28.6% to 32.3%.The results showed that the highest XOS mass concentration was 1.85 g/L after 10 g/L NaOH-ethanol pretreatment, which was 122.89% higher than that of untreated sugarcane bagasse (0.83 g/L).
      ConclusionIn the process of XOS preparation from sugarcane bagasse, enzymatic hydrolysis was an efficient way to achieve the conversion from hemicellulose to XOS. NaOH-ethanol pretreatment can effectively increase the efficiency of enzymatic hydrolysis and promote the preparation of XOS.

       

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