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    苗禹博, 方攀, 杨志恒, 朱晓梅, 高琼, 刘洋, 李伟. 不同地理环境下的樟子松遗传结构分析[J]. 北京林业大学学报, 2018, 40(10): 43-50. DOI: 10.13332/j.1000-1522.20170438
    引用本文: 苗禹博, 方攀, 杨志恒, 朱晓梅, 高琼, 刘洋, 李伟. 不同地理环境下的樟子松遗传结构分析[J]. 北京林业大学学报, 2018, 40(10): 43-50. DOI: 10.13332/j.1000-1522.20170438
    Miao Yubo, Fang Pan, Yang Zhiheng, Zhu Xiaomei, Gao Qiong, Liu Yang, Li Wei. Genetic structure analysis of Pinus sylvestris var. mongolica under different geographical environments[J]. Journal of Beijing Forestry University, 2018, 40(10): 43-50. DOI: 10.13332/j.1000-1522.20170438
    Citation: Miao Yubo, Fang Pan, Yang Zhiheng, Zhu Xiaomei, Gao Qiong, Liu Yang, Li Wei. Genetic structure analysis of Pinus sylvestris var. mongolica under different geographical environments[J]. Journal of Beijing Forestry University, 2018, 40(10): 43-50. DOI: 10.13332/j.1000-1522.20170438

    不同地理环境下的樟子松遗传结构分析

    Genetic structure analysis of Pinus sylvestris var. mongolica under different geographical environments

    • 摘要:
      目的本研究旨在探究樟子松在不同地理环境下的生态适应性问题, 了解遗传多样性与环境因子之间的关系, 在群体遗传结构层面揭示樟子松林分衰退现象。
      方法本文以红花尔基(HHEJ)种源天然林和章古台(ZGT)、围场(WC)、榆林(YL)3个人工引种林, 共计4个樟子松群体为研究对象, 利用SSR分子标记方法对樟子松不同群体的遗传多样性、遗传平衡、遗传结构稳定性、遗传距离以及影响遗传变异主要因素进行了系统分析。
      结果结果表明:4个群体的遗传多样性大小由低到高依次为HHEJ、ZGT、WC、YL; 只有YL群体符合哈迪-温伯格平衡, ZGT群体的连锁不平衡位点达到128对, 偏离哈迪-温伯格平衡的程度最大; HHEJ和ZGT群体之间的遗传距离最为接近, WC群体相距较远, YL群体的遗传距离最远; 4个樟子松群体的遗传分化相对稳定, 不易产生遗传分化; 多元回归分析显示樟子松期望杂合度(He)与地理纬度(La)具有显著的负相关关系(r=-0.957), 线性关系在α=0.05的水平上显著(P=0.012)。
      结论本研究发现樟子松的遗传多样性可能随着纬度的升高而降低, 阐明了不同地区樟子松群体的遗传差异情况, 及其林分衰退的可能因素, 为樟子松科学的遗传资源保护、引种造林提供了数据支撑和理论指导。

       

      Abstract:
      ObjectiveThis paper aims to study the ecological adaptability of Pinus sylvestris var. mongolica in different geographical environments, to explore the relationship between genetic diversity and environmental factors, and to reveal the forest deterioration of Pinus sylvestris var. mongolica at population level.
      MethodIn this paper, four Pinus sylvestris var. mongolica populations of Honghuaerji, Zhanggutai, Weichang and Yulin were selected as the study objects, the genetic diversity, genetic balance, genetic structure stability, genetic distance and the main factors affecting genetic variation in this four different populations were analyzed systematically and comprehensively by SSR molecular marker method.
      ResultThe results showed that the genetic diversity of the four populations ranked as Yulin, Weichang, Zhanggutai, Honghuaerji; only the Yulin population was in accordance with the Hardy-Weinberg equilibrium, and the linkage disequilibrium points of the Zhanggutai population reached 128 pairs, deviating from Hardy-Weinberg equilibrium at P < 0.05 level; the genetic distance between Honghuaerji and Zhanggutai populations was closer, followed by Weichang population and the furthest was Yulin population; the four populations had relatively stable genetic differentiation, and were not prone to genetic differentiation; multiple regression analysis showed that the expected heterozygosity (He) had a significant negative correlation with geographic latitude (La) (r=-0.957), and the linear relationship was significant at P < 0.05 level.
      ConclusionThe genetic diversity of Pinus sylvestris var. mongolica may decreased with the increase of latitude. This study improved the genetic diversity and genetic structure of Pinus sylvestris var. mongolica at the population level, clarified the genetic diversity inter or inner population, complemented the cause of forest deterioration, which provided a theoretical basis for the genetic resource protection and the introduction and popularization of Pinus sylvestris var. mongolica in large area.

       

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