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落叶松毛虫对茉莉酸甲酯处理长白落叶松苗的行为反应

孟昭军 林健 王琪 鲁艺芳 王雷 严善春

孟昭军, 林健, 王琪, 鲁艺芳, 王雷, 严善春. 落叶松毛虫对茉莉酸甲酯处理长白落叶松苗的行为反应[J]. 北京林业大学学报, 2018, 40(12): 60-67. doi: 10.13332/j.1000-1522.20180036
引用本文: 孟昭军, 林健, 王琪, 鲁艺芳, 王雷, 严善春. 落叶松毛虫对茉莉酸甲酯处理长白落叶松苗的行为反应[J]. 北京林业大学学报, 2018, 40(12): 60-67. doi: 10.13332/j.1000-1522.20180036
Meng Zhaojun, Lin Jian, Wang Qi, Lu Yifang, Wang Lei, Yan Shanchun. Behavioral responses of Dendrolimus superans to Larix olgensis seedlings treated with methyl jasmonate[J]. Journal of Beijing Forestry University, 2018, 40(12): 60-67. doi: 10.13332/j.1000-1522.20180036
Citation: Meng Zhaojun, Lin Jian, Wang Qi, Lu Yifang, Wang Lei, Yan Shanchun. Behavioral responses of Dendrolimus superans to Larix olgensis seedlings treated with methyl jasmonate[J]. Journal of Beijing Forestry University, 2018, 40(12): 60-67. doi: 10.13332/j.1000-1522.20180036

落叶松毛虫对茉莉酸甲酯处理长白落叶松苗的行为反应

doi: 10.13332/j.1000-1522.20180036
基金项目: 

林业公益性行业科研专项 200904021

中央高校基本科研业务费专项 DL13BAX31

详细信息
    作者简介:

    孟昭军,副教授。主要研究方向:昆虫化学生态与害虫生物防治。Email: mzj-72@163.com   地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院

    责任作者:

    严善春,教授,博士生导师。主要研究方向:森林昆虫学、昆虫化学生态学、资源昆虫学。Email: yanshanchun@126.com   地址:同上

  • 中图分类号: S763.7

Behavioral responses of Dendrolimus superans to Larix olgensis seedlings treated with methyl jasmonate

  • 摘要: 目的通过外源茉莉酸甲酯(MeJA)处理长白落叶松对落叶松毛虫寄主选择行为影响的研究,明确外源茉莉酸甲酯的诱导落叶松抗虫作用,为生态防治落叶松食叶害虫提供理论依据。方法本实验利用昆虫行为测定法,研究落叶松毛虫对不同浓度茉莉酸甲酯处理2年生长白落叶松苗的行为反应。结果茉莉酸甲酯诱导处理的松苗对落叶松毛虫雌虫具有很强的驱避作用,迫使其将大量卵产在对照处理松苗上;落叶松毛虫在用1、5或10mmol/L MeJA处理松苗上产卵量与对照相比分别减少47.21%~77.03%、43.18%~55.46%或71.80%~80.08%;落叶松毛虫雌虫对1、5或10mmol/L MeJA处理松苗的嗅觉反应率分别比对照降低49.24%、59.71%或43.68%~61.24%;而高浓度茉莉酸甲酯处理对落叶松毛虫嗅觉及产卵选择的影响更强。结论茉莉酸甲酯处理的长白落叶松明显影响落叶松毛虫雌虫的产卵和嗅觉选择行为,且存在浓度依赖关系,诱导影响持续3~5d。

     

  • 图  1  组1罩笼试验松苗摆放及放虫示意图

    A.落叶松毛虫;B.长白落叶松苗。

    Figure  1.  Diagram of the seedling setting and the released Dendrolimus superans in group 1

    A, Dendrolimus superans; B, Larix olgensis seedlings.

    图  2  落叶松毛虫成虫产卵选择试验示意图

    松苗处理后 After the seedlings treated with MeJA; 放苗 Putting the seedlings into cages, 放成虫 Releasing the adult Dendrolimus superans ionto the cages, 产卵 Female Dendrolimus superans laying eggs on the seedlings; 调查 Checking the number of eggs on the seedlings, 取苗、取成虫 Moving away the seedlings, the adult Dendrolimus superans in the cages, 取卵 Moving away eggs on the seedlings and cages; 第1、2、3次 The first, second, third time; 苗虫 Seedlings and moths

    Figure  2.  Diagram of the oviposition choice test of the adult Dendrolimus superans

    图  3  落叶松毛虫嗅觉生测试验示意图

    吸附剂管 Adsorbent tube; 松苗 Larix olgensis seedling; 有机玻璃罩 Perspex hood; 塑料桶 Plastic can; 松毛虫引入口 Entrance of Dendrolimus superans; 气流方向 Airflow direction; 大气采样仪 Atmospheric sampling apparatus; Y形管嗅觉仪 Y-tube olfactometers; 盛水大盆 Big basin with water

    Figure  3.  Diagram of the olfactory choice test of the adult Dendrolimus superans

    图  4  落叶松毛虫对不同浓度茉莉酸甲酯处理长白落叶松的产卵选择(组1)

    CK.对照。同一天不同组合中,柱形图上不同小写字母表示在P<0.05水平上差异显著,不同大写字母表示在P<0.01水平上差异显著。下同。

    Figure  4.  Oviposition selection of D. superans adults to L. olgensis seedlings treated with different concentrations of MeJA (group 1)

    CK, control. Different lowercases on the bar charts mean significant difference (P < 0.05), and different upper cases on them indicate significant difference (P < 0.01) in the same time and different groups. The same below.

    图  5  落叶松毛虫对不同浓度茉莉酸甲酯处理长白落叶松的产卵选择(组2)

    Figure  5.  Oviposition selection of D. superans adults to L. olgensis seedlings treated with different concentrations of MeJA (group 2)

    图  6  落叶松毛虫对不同浓度茉莉酸甲酯处理长白落叶松的嗅觉反应(组1)

    Figure  6.  Olfactory responses of D. superans adults to L. olgensis seedlings treated with different concentrations of MeJA (group 1)

    图  7  落叶松毛虫对不同浓度茉莉酸甲酯处理长白落叶松的嗅觉反应(组2)

    Figure  7.  Olfactory responses of D. superans adults to L. olgensis seedlings treated with different concentrations of MeJA (group 2)

    表  1  不同浓度茉莉酸甲酯处理长白落叶松苗的方法

    Table  1.   Method of treating larch seedlings with different concentrations of methyl jasmonate

    组别
    Group
    处理
    Treatment
    浓度
    Concentration/
    (mmoL·L-1)
    用量/(mL·株-1)
    Dosage/
    (mL·plant-1)
    质量/(mg·株-1)
    Mass/
    (mg·plant-1)
    组1 Group 1对照Control050
    1 mmoL/L茉莉酸甲酯1 mmoL/L methyl jasmonate(MeJA1)151.12
    5 mmoL/L茉莉酸甲酯5 mmoL/L methyl jasmonate(MeJA5)555.61
    组2 Group 2对照Control050
    1 mmoL/L茉莉酸甲酯1 mmoL/L methyl jasmonate(MeJA1)151.12
    10 mmoL/L茉莉酸甲酯10 mmoL/L methyl jasmonate(MeJA10)10511.22
    注:各处理溶液用含5%乙醇和0.1% Tween20水溶液配制;对照为含5%乙醇和0.1% Tween20(V/V)的水溶液。Notes: different treatment solution was made up with the water solution including 5% ethanol and 0.1% Tween20. Control was the water solution including 5% ethanol and 0.1% Tween20.
    下载: 导出CSV
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  • 收稿日期:  2018-01-24
  • 修回日期:  2018-03-27
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