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传粉综合征预测传粉者准确性检验——以邱北冬蕙兰为例

胡世保 辛荣仕 郭红艳 王晓洁 张自斌 程瑾

胡世保, 辛荣仕, 郭红艳, 王晓洁, 张自斌, 程瑾. 传粉综合征预测传粉者准确性检验——以邱北冬蕙兰为例[J]. 北京林业大学学报, 2018, 40(6): 101-110. doi: 10.13332/j.1000-1522.20180055
引用本文: 胡世保, 辛荣仕, 郭红艳, 王晓洁, 张自斌, 程瑾. 传粉综合征预测传粉者准确性检验——以邱北冬蕙兰为例[J]. 北京林业大学学报, 2018, 40(6): 101-110. doi: 10.13332/j.1000-1522.20180055
Hu Shibao, Xin Rongshi, Guo Hongyan, Wang Xiaojie, Zhang Zibin, Cheng Jin. Accuracy detection of predicting pollinator from pollination syndromes: taking Cymbidium qiubeiense as an example[J]. Journal of Beijing Forestry University, 2018, 40(6): 101-110. doi: 10.13332/j.1000-1522.20180055
Citation: Hu Shibao, Xin Rongshi, Guo Hongyan, Wang Xiaojie, Zhang Zibin, Cheng Jin. Accuracy detection of predicting pollinator from pollination syndromes: taking Cymbidium qiubeiense as an example[J]. Journal of Beijing Forestry University, 2018, 40(6): 101-110. doi: 10.13332/j.1000-1522.20180055

传粉综合征预测传粉者准确性检验——以邱北冬蕙兰为例

doi: 10.13332/j.1000-1522.20180055
基金项目: 

国家林业局野生植物保护管理项目 20160401

国家自然科学基金项目 31000202

广西创新驱动发展专项资金项目 桂科AA17204045-6

详细信息
    作者简介:

    胡世保。主要研究方向:兰科植物传粉生物学。Email:2446406009@qq.com 地址:100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    程瑾,博士,副教授。主要研究方向:兰科植物繁殖生态学。Email:chengjin@bjfu.edu.cn 地址:同上

  • 中图分类号: S682.31; Q944.43

Accuracy detection of predicting pollinator from pollination syndromes: taking Cymbidium qiubeiense as an example

  • 摘要: 目的传粉综合征理论为植物与传粉者相互关系的进化提供了经典的假说, 然而, 随着越来越多泛化传粉系统被报道, 基于传粉综合征预测传粉者的准确性受到质疑。邱北冬蕙兰隶属于兰属建兰亚属, 花具香味, 唇瓣具紫色斑点。这些形态特征与已报道的由中华蜜蜂传粉的多种建兰亚属植物, 如兔耳兰、春兰等相似。我们根据传粉综合征理论预测邱北冬蕙兰传粉者同样为中华蜜蜂。本研究将验证利用传粉综合征预测传粉者这一方法的准确性, 探讨其适用范围。方法以邱北冬蕙兰为实验材料, 于2010—2012年在广西雅长兰科植物国家级自然保护区开展系统的传粉生物学研究, 包括开花物候观测、花形态测量、昆虫传粉行为观察、花颜色测量、花气味分析及人工繁育系统实验。结果中华蜜蜂的工蜂是邱北冬蕙兰唯一的传粉者, 花内不存在任何报酬物质, 推测具食源性欺骗传粉机制。唇瓣紫色斑点作为假蜜导欺骗中华蜜蜂访花, 花气味中含有酚类、酯类、醛类等物质, 其中醛类物质可能在吸引中华蜜蜂传粉过程中起着重要的作用, 利用传粉综合征预测传粉者的准确性得到成功检验。结论本研究为传粉综合征能够准确地预测传粉者的科学假设提供研究案例。但由于传粉综合征是由一组动态的、进化的花部性状组成, 通过传粉综合征预测传粉者时, 在已知亲缘关系相近植物传粉者前提下还需注意到生境变化、次级传粉者、传粉者功能群、历史演化、基因等因素的影响。

     

  • 图  1  邱北冬蕙兰的传粉者和花部结构

    A.邱北冬蕙兰传粉者中华蜜蜂;Po.花粉块;B.传粉者进入花内;C.邱北冬蕙兰的花部结构

    Figure  1.  Pollinator and flower structure of C. qiubeiense

    A, pollinator A.cerana cerana of C.qiubeiense, taking the pollinia on its mid-thorax; Po, pollinia; B, entering flower process of A.cerana cerana; C, flower structure of C.qiubeiense

    图  2  邱北冬蕙兰和兔耳兰唇瓣和叶片颜色光谱反射率曲线

    C.qiubeiense [P]为邱北冬蕙兰唇瓣斑点;C.qiubeiense [W]为邱北冬蕙兰唇瓣非斑点;C.qiubeiense [L]为邱北冬蕙兰叶片;C.lancifolium [P]为兔耳兰唇瓣斑点;C.lancifolium [W]为兔耳兰唇瓣非斑点;C.lancifolium [L]为兔耳兰叶片。下同。

    Figure  2.  Reflectance of labellum and leaf in C.qiubeiense and C.lancifolium

    C.qiubeiense [P], the purplish spots on the labellum of C.qiubeiense; C.qiubeiense [W], the white area on the labellum of C.qiubeiense; C.qiubeiense [L], the leaf of C.qiubeiense; C.lancifolium [P], the purplish spots on the labellum of C.lancifolium; C.lancifolium [W], the white area on the labellum of C.lancifolium; C.lancifolium [L], the leaf of C.lancifolium. The same below.

    图  3  邱北冬蕙兰和兔耳兰唇瓣在蜂类视觉颜色六边形的颜色位点

    Figure  3.  Color loci on the labellum of C. qiubeiense and C. lancifolium in the bee color hexagon

    表  1  邱北冬蕙兰花气味化学成分GC-MS鉴定结果及兔耳兰中对中华蜜蜂起作用的化学成分

    Table  1.   Compounds in floral odor of C. qiubeiense by GC-MS and the compounds acting on A. cerana cerana in floral odor of C. lancifolium

    化合物
    Compounds
    邱北冬蕙兰花气味中化学成分
    Compounds in floral odor of C. qiubeiense
    兔耳兰花气味中对中华蜜蜂起作用的化学成分
    Compounds acting on A. cerana cerana in floral odor of C. lancifolium
    相对含量
    Relative content/%
    保留指数
    Retention index
    相对含量
    Relative content/%
    6-甲基-5-庚烯-2-酮5-Hepten-2-one, 6-methyl- 3.80 988
    辛醛Octanal 2.31 1005
    环己酮Cyclohexanone 3.56
    2-乙基-1-己醇1-Hexanol, 2-ethyl- 2.27 1030
    壬醛Nonanal 13.16 1106 1.51
    乙酸苯甲酯Acetic acid, phenylmethyl ester 0.12 1168 17.66
    反式-2-十二烯-1-醇trans-2-Dodecen-1-ol 1.44 1172
    十二烷Dodecane 0.73 1 201
    癸醛Decanal 7.90 1 209
    十五烷Pentadecane 2.04 1 282
    (1, 1′-联环戊基)-2-酮[1, 1′-Bicyclopentyl]-2-one 2.15 1292
    十一醛Undecanal 0.84 1309
    十二醛Dodecanal 0.91 1411
    乙酸-2-十烯酯(E)-2-Decenyl acetate 1.39
    苯甲醇Benzyl Alcohol 0.46
    6, 10-二甲基-5, 9-十一烷二烯-2-酮5, 9-Undecadien-2-one, 6, 10-dimethyl 3.15 1457
    2, 6, 10-三甲基-十四烷Tetradecane, 2, 6, 10-trimethyl- 0.44 1464
    2, 6-二叔丁基-1, 4-苯二酚1, 4-Benzenediol, 2, 6-bis(1, 1-dimethylethyl)- 0.49 1476
    2, 4-二叔丁基-苯酚Phenol, 2, 4-bis(1, 1-dimethylethyl)- 30.67 1515
    6-莰烯醇6-Camphenol 2.91 1526
    2-甲基-二十烷Eicosane, 2-methyl- 0.30 1554
    十六烷Hexadecane 1.52 1601
    十八烷Octadecane 1.13 1800
    肉豆蔻酸异丙酯Isopropyl myristate 16.16 1830
    二十七烷Heptacosane 0.97 2 143
    3, 4, 5, 6′-四叔丁基-2, 3′-联苯二酚3, 4, 5, 6′-tetra-tert-butylbiphenyl-2, 3′-diol 4.59 2369
    总和Total 100.00 24.58
    下载: 导出CSV

    表  2  邱北冬蕙兰人工授粉实验结实情况

    Table  2.   Fruit sets of different hand-pollination treatments of C. qiubeiense

    实验处理
    Experimental treatment
    实验花数
    Flower number in the experiment
    结实数目
    Fruit number
    结实率
    Seed setting rate/%
    2010 2011 2010 2011 2010 2011
    自动自花授粉Spontaneous self-pollination 12 12 0 0 0 0
    人工自交Self-pollination 12 12 11 11 91.66 91.66
    同株异花授粉Geitonogamy (pollinia from the same inflorescence) 12 12 12 11 100.00 91.66
    同克隆授粉Geitonogamy (pollinia from the same clone) 12 12 10 9 83.33 75.00
    人工异交Cross-pollination 12 12 12 11 100.00 91.66
    无融合生殖Apomixis 12 12 0 0 0 0
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-03-05
  • 修回日期:  2018-05-09
  • 刊出日期:  2018-06-01

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