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    姜贵全, 张卓睿, 张诗朦, 任宣百, 庞久寅. 落叶松树皮多聚原花青素的树脂催化降解及抗氧化活性[J]. 北京林业大学学报, 2018, 40(9): 118-126. DOI: 10.13332/j.1000-1522.20180124
    引用本文: 姜贵全, 张卓睿, 张诗朦, 任宣百, 庞久寅. 落叶松树皮多聚原花青素的树脂催化降解及抗氧化活性[J]. 北京林业大学学报, 2018, 40(9): 118-126. DOI: 10.13332/j.1000-1522.20180124
    Jiang Guiquan, Zhang Zhuorui, Zhang Shimeng, Ren Xuanbai, Pang Jiuyin. Degradation of polymeric proanthocyanidin from larch bark catalyzed by resin and antioxidant activity[J]. Journal of Beijing Forestry University, 2018, 40(9): 118-126. DOI: 10.13332/j.1000-1522.20180124
    Citation: Jiang Guiquan, Zhang Zhuorui, Zhang Shimeng, Ren Xuanbai, Pang Jiuyin. Degradation of polymeric proanthocyanidin from larch bark catalyzed by resin and antioxidant activity[J]. Journal of Beijing Forestry University, 2018, 40(9): 118-126. DOI: 10.13332/j.1000-1522.20180124

    落叶松树皮多聚原花青素的树脂催化降解及抗氧化活性

    Degradation of polymeric proanthocyanidin from larch bark catalyzed by resin and antioxidant activity

    • 摘要:
      目的研究落叶松树皮多聚原花青素的树脂催化降解工艺及降解产物的抗氧化活性,为落叶松树皮的开发利用提供科学依据。
      方法以落叶松树皮中多聚原花青素(LPPC)为原料,使用CR57树脂催化降解制备了落叶松树皮低聚原花青素(LOPC)。通过单因素试验和响应面试验分析,优化降解工艺条件,并对降解产物进行结构表征,考察其抗氧化能力。
      结果催化降解最佳工艺条件为每100mL多聚原花青素中加入18mL催化树脂,催化温度76℃,催化时间1.8h,此条件下降解产物平均聚合度为3.76。对比LPPC和降解产物LOPC的色谱图,发现降解产物中多聚组分含量明显降低,证实LPPC发生了降解反应;根据紫外光谱图和红外光谱图,确定降解产物具有原花青素的结构特征;由质谱图可知降解产物是从三聚体到十二聚体,并以四聚体为主分布的低聚物。分析降解产物的抗氧化活性,其1, 1-二苯基-2-三硝基苯肼(DPPH)自由基、羟自由基(·OH)和2, 2′-二氮-双(3-乙基苯并噻唑-6-磺酸)铵盐(ABTS)自由基的半数抑制质量浓度(IC50)分别为72.56、12.64和2.52mg/L。与各对照品相比较,降解产物清除DPPH自由基的能力最强,其还原能力、清除·OH自由基能力和清除ABTS自由基能力仅低于葡萄籽提取物(GS),而高于松树皮提取物(PB)、VC、特丁基对苯二酚(TBHQ)。
      结论催化树脂CR57可适用于催化LPPC的降解反应,降解后的LOPC具有较强的抗氧化性。

       

      Abstract:
      ObjectiveIn order to provide scientific basis for development and utilization of larch bark, we investigated the degradation conditions of polymeric proanthocyanidin from larch bark catalyzed by resin and antioxidant activity of degradation products.
      MethodLarch bark oligomeric proanthocyanidins (LOPC) was prepared from polymeric proanthocyanidins of larch bark (LPPC) by CR57 resin catalytic degradation. Based on single factor experiments and response surface design analysis, the degrading conditions were optimized, and the structure characterization and antioxidant activity of the degradation product were analyzed as well.
      ResultThe results showed that the optimum conditions of catalytic degradation were as follows: the catalytic resin dosage 18mL per 100mL LPPC, catalytic temperature 76 ℃, catalytic reaction time 1.8 hours. Under the above conditions, the average degree of polymerization of degradation products was 3.76. Through the comparison of the chromatogram of LPPC and LOPC, it was found that the content of polymeric proanthocyanidins in the degradation products was obviously reduced, thereby proving that LPPC was degraded. The degradation products were characterized by UV and FTIR, and confirmed to have procyanidins features. The molecular mass distribution of the degradation products was analyzed by the linear model of MALDI-TOF mass spectrometry. The results revealed that LOPC was an oligomer with tetramer mainly from trimer to twelve oligomer. The LOPC exhibited a strong antioxidant activity, and its IC50 on DPPH free radical scavenging, ·OH free radical scavenging and ABTS free radical scavenging were 72.56, 12.64 and 2.52mg/L, respectively. Compared with GS, the LOPC on DPPH· scavenging capacity was higher and the reducing capacity, ·OH scavenging capacity and ABTS+· scavenging capacity were lower. But the LOPC on these free radical scavenging capacity was higher than PB, VC, TBHQ.
      ConclusionIt was concluded that CR57 resin could be used to catalyze the degradation reaction of LPPC, and the degradation product LOPC had good antioxidant activity.

       

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