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    张永卓, 刘雅琳, 陆海, 李慧. 转录组分析组蛋白去乙酰化酶抑制剂TSA对毛白杨悬浮细胞表达谱的影响[J]. 北京林业大学学报, 2018, 40(9): 1-14. DOI: 10.13332/j.1000-1522.20180158
    引用本文: 张永卓, 刘雅琳, 陆海, 李慧. 转录组分析组蛋白去乙酰化酶抑制剂TSA对毛白杨悬浮细胞表达谱的影响[J]. 北京林业大学学报, 2018, 40(9): 1-14. DOI: 10.13332/j.1000-1522.20180158
    Zhang Yongzhuo, Liu Yalin, Lu Hai, Li Hui. Transcriptome analysis of gene expression profiling in the suspension cells of Populus tomentosa treated by histone deacetylase inhibitor TSA[J]. Journal of Beijing Forestry University, 2018, 40(9): 1-14. DOI: 10.13332/j.1000-1522.20180158
    Citation: Zhang Yongzhuo, Liu Yalin, Lu Hai, Li Hui. Transcriptome analysis of gene expression profiling in the suspension cells of Populus tomentosa treated by histone deacetylase inhibitor TSA[J]. Journal of Beijing Forestry University, 2018, 40(9): 1-14. DOI: 10.13332/j.1000-1522.20180158

    转录组分析组蛋白去乙酰化酶抑制剂TSA对毛白杨悬浮细胞表达谱的影响

    Transcriptome analysis of gene expression profiling in the suspension cells of Populus tomentosa treated by histone deacetylase inhibitor TSA

    • 摘要:
      组蛋白去乙酰化酶HDAC具有调节细胞增殖、诱导细胞分化、凋亡的作用, 是近年生物学研究热点之一, 而在木本植物方面的作用却鲜有报道。
      目的本实验用组蛋白去乙酰化酶抑制剂曲古抑菌素A(Trichostatin A, TSA)对杨树悬浮细胞进行处理, 通过改变细胞的组蛋白乙酰化水平, 来探讨其对基因表达谱变化的影响。
      方法用0.5 μmol/L的TSA处理悬浮细胞, 10 d后收集材料用于RNA-Seq分析, 比较经过TSA处理与对照组的悬浮细胞之间转录组表达差异情况。
      结果通过显微镜观察发现, 对照组细胞呈均匀椭圆形, 而经过TSA处理后的细胞出现圆形及长条形细胞。通过转录组分析得到4 465个差异表达基因(DEGs), 其中2 363个基因上调, 2 102个基因下调。差异表达基因主要富集在细胞周期、苯丙素生物合成、细胞壁结构成分和乙酰化相关等途径。
      结论通过分析TSA处理和对照组的悬浮细胞基因表达差异, 发现TSA通过影响组蛋白乙酰化水平直接或间接影响细胞生长、细胞壁组分和细胞周期的相关基因, 为认识组蛋白乙酰化对植物生长发育的影响提供依据。

       

      Abstract:
      Histone deacetylase (HDAC), which regulates cell proliferation, cell diferentiation and apoptosis, is one of the hot topics in biology in recent years, but rarely reported in woody plants.
      ObjectiveIn this study, the change of gene expression profile was investigated by treating the poplar suspension cells with TSA to change the level of histone acetylation in cells.
      MethodThe suspension cells were treated with 0.5 μmol/L TSA for ten days and were collected for RNA-Seq analysis. Then the gene expression differences were compared between TSA-treated cells and control group suspension cells.
      ResultThe cells in the control group were homogeneous and oval, while the cells after TSA treatment were round and elongated by microscope observation. 4 465 differentially expressed genes (DEGs) were obtained by the transcriptome analysis, of which 2 363 genes were up-regulated and 2 102 genes were down-regulated. Differentially expressed genes were mainly enriched in cell cycle, phenylpropanoid biosynthesis, cell wall structure and acetylation.
      ConclusionBy analyzing the difference of gene expression between TSA-treated and control group suspension cells, our results suggest that TSA can directly or indirectly affect genes that regulate cell growth, cell wall structure and lignin synthesis pathway through affecting the level of histone acetylation, which provide evidence for understanding of histone acetylation on plant growth and development.

       

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