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LdNPV对CO2胁迫下舞毒蛾生长发育及生化酶活性影响

李欣悦 王振越 曹传旺

李欣悦, 王振越, 曹传旺. LdNPV对CO2胁迫下舞毒蛾生长发育及生化酶活性影响[J]. 北京林业大学学报, 2019, 41(9): 139-146. doi: 10.13332/j.1000-1522.20180199
引用本文: 李欣悦, 王振越, 曹传旺. LdNPV对CO2胁迫下舞毒蛾生长发育及生化酶活性影响[J]. 北京林业大学学报, 2019, 41(9): 139-146. doi: 10.13332/j.1000-1522.20180199
Li Xinyue, Wang Zhenyue, Cao Chuanwang. Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO2 concentration stress[J]. Journal of Beijing Forestry University, 2019, 41(9): 139-146. doi: 10.13332/j.1000-1522.20180199
Citation: Li Xinyue, Wang Zhenyue, Cao Chuanwang. Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO2 concentration stress[J]. Journal of Beijing Forestry University, 2019, 41(9): 139-146. doi: 10.13332/j.1000-1522.20180199

LdNPV对CO2胁迫下舞毒蛾生长发育及生化酶活性影响

doi: 10.13332/j.1000-1522.20180199
基金项目: 国家重点研发计划(2018YFC1200400),国家自然科学基金项目(31570642)
详细信息
    作者简介:

    李欣悦。主要研究方向:昆虫生理生化与分子毒理学。Email:lixinyue62@163.com 地址:150040黑龙江省哈尔滨市和兴路26号东北林业大学林学院

    责任作者:

    曹传旺,博士,教授。主要研究方向:昆虫生理生化与分子毒理学。Email:chuanwangcao@126.com 地址:同上

  • 中图分类号: S763.301

Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO2 concentration stress

  • 摘要: 目的以森林害虫舞毒蛾为对象,研究CO2介导下LdNPV病毒对舞毒蛾生长发育、解毒酶和保护酶的影响。方法利用密闭式CO2人工气候箱研究了不同CO2含量(397、550和750 μL/L)条件下饲养的舞毒蛾幼虫,LdNPV对其生长发育指标的影响,并采用分光光度计法测定体内保护酶和解毒酶活性。结果高CO2含量胁迫下舞毒蛾3龄幼虫体重累计增长率降低。LdNPV胁迫导致不同CO2含量(397、550和750 μL/L)条件下饲养的舞毒蛾3龄幼虫体重累计增长率分别比对照组增加81.27%、71.63%和68.41%;随着CO2含量升高幼虫感染LdNPV死亡率增加,750 μL/L高含量处理组死亡率为27.09%。高CO2含量胁迫下舞毒蛾3龄幼虫体内解毒酶CarE和AChE活性随着CO2含量升高而诱导增加,ALP活性随CO2含量增加抑制下降;保护酶CAT活性随着CO2含量升高诱导增加,而SOD活性随着CO2含量升高抑制减少。高CO2含量条件下生长的舞毒蛾3龄幼虫接种LdNPV后,随着CO2含量升高体内CarE、ALP、AChE和CAT活性被抑制,而SOD活性表现为诱导增加。结论不同CO2含量下舞毒蛾饲养至3龄幼虫接种LdNPV,随着CO2含量增加,幼虫体重累计增长率减少、死亡率增加。舞毒蛾幼虫CarE、AChE、ALP和CAT活性随着LdNPV处理时间和CO2含量增加而主要表现为抑制减少;SOD活性则主要表现为诱导增加。CO2含量升高可能通过影响生长发育和生理生化增加LdNPV对舞毒蛾幼虫的致病力。

     

  • 图  1  不同CO2含量下舞毒蛾3龄幼虫喂食LdNPV病毒后累计死亡率

    不同小写字母表示同一时间不同处理组的差异显著性(P < 0.05)。下同。Different lowercase letters indicate significant differences in same time among varied treatment groups (P < 0.05). The same below.

    Figure  1.  Cumulative mortality of 3rd instar L. dispar larvae after feeding LdNPV virus

    图  2  LdNPV对不同CO2含量胁迫下舞毒蛾3龄幼虫体内CarE、ALP和AChE活性影响

    Figure  2.  Effects of LdNPV on CarE, ALP and AChE activities in 3rd instar L. dispar larvae under different CO2 concentration stress

    图  3  LdNPV对不同含量CO2下舞毒蛾3龄幼虫体内CAT和SOD活性影响

    Figure  3.  Effects of LdNPV on CAT and SOD activities in 3rd instar L. dispar larvae under different CO2 concentration stresses

    表  1  LdNPV对舞毒蛾3龄幼虫毒力

    Table  1.   Toxicity of LdNPV to 3rd instar L. dispar larvae under CO2 concentration stresses

    LC50(95% 置信区间)
    LC50 (95% confidence interval)/(PIB·μL− 1)
    LC20(95%置信区间)
    LC20 (95% confidence interval)//(PIB·μL− 1)
    斜率
    Slope
    χ2 (df)
    754 (504 ~ 1 098)11 (0.79 ~ 55.00)2.20 ± 0.4523 (22)
    下载: 导出CSV

    表  2  LdNPV对CO2含量胁迫下舞毒蛾3龄幼虫发育历期影响

    Table  2.   Effects of LdNPV on the developmental period of 3rd instar L. dispar larvae under CO2 concentration stress d

    非LdNPV侵染处理 Non-LdNPV infection treatmentLdNPV侵染处理 LdNPV infection treatment
    397 CK550 CK750 CK397 NPV550 NPV750 NPV
    5.10 ± 1.41a5.50 ± 1.32a5.7 ± 0.83a6.35 ± 1.43a6.13 ± 0.80a6.50 ± 1.20a
    注:397 CK、550 CK和750 CK为蒸馏水浸湿的人工饲料饲喂397、550和750 μL/L CO2含量处理后舞毒蛾幼虫;397 NPV、550 NPV和750 NPV为LdNPV浸湿的人工饲料饲喂397、550和750 μL/L CO2含量处理后舞毒蛾幼虫。数据为平均值 ± 标准误,相同小写字母表示差异性不显著(P > 0.05),下同。Notes: 397 CK, 550 CK and 750 CK are L. dispar larvae fed on artificial diets soaked by distilled water after 397, 550 and 750 μL/L CO2 treatments, respectively. 397 NPV, 550 NPV and 750 NPV are L. dispar larvae fed on artificial diets soaked by LdNPV after 397, 550 and 750 μL/L CO2 treatments, respectively. The data in table is mean ± standard error. The same lowercase letters in a column indicate no significant difference at 0.05 level. The same below.
    下载: 导出CSV

    表  3  感染LdNPV舞毒蛾幼虫体重累计增长率

    Table  3.   Cumulative growth rate of L. dispar larvae after infection by LdNPV

    处理
    Treatment
    指标
    Index
    饲喂天数 Feeding days
    1234567
    397 CK 幼虫鲜质量 Larva fresh mass/mg 4.56 ± 0.25Be 7.34 ± 0.71Bd 8.08 ± 1.48Bd 8.57 ± 2.54Bd 9.18 ± 2.91Cc 11.64 ± 1.97Ab 13.80 ± 2.40Aa
    累计增长率 Cumulative increase rate/% 60.83 ± 9.63Be 76.62 ± 7.72Bd 86.72 ± 13.87Acd 99.55 ± 6.22Ac 152.75 ± 3.25Ab 200.24 ± 5.07Aa
    397 NPV 幼虫鲜质量 Larva fresh mass/mg 4.58 ± 0.36Bd 5.70 ± 0.42Cc 6.75 ± 0.55Bb 7.33 ± 0.89BCab 7.66 ± 0.73Dab 7.72 ± 1.27Cab 8.22 ± 0.51Da
    累计增长率 Cumulative increase rate/% 25.61 ± 15.73Dd 49.15 ± 4.84Cc 62.50 ± 4.64Bb 69.70 ± 7.73Cb 71.87 ± 7.43Cb 81.27 ± 13.01Ca
    550 CK 幼虫鲜质量 Larva fresh mass/mg 5.59 ± 0.29Ad 9.84 ± 0.75Ac 10.64 ± 0.85Abc 10.82 ± 1.08Ab 11.12 ± 0.79Ab 11.20 ± 1.68Ab 12.44 ± 2.36Ba
    累计增长率 Cumulative increase rate/% 75.93 ± 9.47Ac 90.02 ± 5.15Abc 93.04 ± 2.57Abc 98.68 ± 16.27Ab 99.35 ± 9.48Bb 124.25 ± 31.26Ba
    550 NPV 幼虫鲜质量 Larva fresh mass/mg 5.68 ± 0.28Ab 9.61 ± 0.62Aa 9.99 ± 0.83Aa 10.15 ± 0.74Aa 10.14 ± 0.79Ba 9.83 ± 0.39Ba 9.75 ± 0.56Ca
    累计增长率 Cumulative increase rate/% 69.23 ± 8.10ABc 75.91 ± 2.39Bab 78.70 ± 1.89Ba 78.55 ± 2.78Ba 73.30 ± 3.64Cbc 71.63 ± 2.34Cbc
    750 CK 幼虫鲜质量 Larva fresh mass/mg 4.51 ± 0.20Bc 6.21 ± 0.13BCb 6.60 ± 0.63Bab 6.27 ± 0.41Cb 6.75 ± 0.90Dab 7.46 ± 0.40Ca 7.70 ± 1.63Da
    累计增长率 Cumulative increase rate/% 37.82 ± 4.83Cc 46.43 ± 7. 89Cb 39.08 ± 5.04Cc 49.50 ± 8.0Db 65.83 ± 11.58CDa 70.50 ± 16.48Ca
    750 NPV 幼虫鲜质量 Larva fresh mass/mg 4.96 ± 0.80ABb 7.19 ± 1.27Ba 7.32 ± 1.41Ba 7.16 ± 1.04BCa 6.95 ± 0.60Da 7.73 ± 1.05Ca 8.14 ± 0.43Da
    累计增长率 Cumulative increase rate/% 44.85 ± 5.33Cc 47.06 ± 2.31Cc 45.17 ± 5.94Cc 41.84 ± 7.86Dc 56.50 ± 5.27Db 68.41 ± 14.42Da
    注:表中数据为平均值 ± 标准误,不同小写字母表示同一组不同天数差异显著性,不同大写字母表示同一天不同组差异显著性(P < 0.05)。Notes: data in table is mean ± standard error. Different lowercase letters indicate significant difference in the same group of different days. Different capital letters indicate significant differences of different groups on the same day (P < 0.05).
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-06-20
  • 修回日期:  2018-09-21
  • 网络出版日期:  2019-07-13
  • 刊出日期:  2019-09-01

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