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高温诱导大孢子染色体加倍选育毛白杨杂种三倍体

耿喜宁 任勇谕 韩志强 杜康 康向阳

耿喜宁, 任勇谕, 韩志强, 杜康, 康向阳. 高温诱导大孢子染色体加倍选育毛白杨杂种三倍体[J]. 北京林业大学学报, 2018, 40(11): 12-18. doi: 10.13332/j.1000-1522.20180215
引用本文: 耿喜宁, 任勇谕, 韩志强, 杜康, 康向阳. 高温诱导大孢子染色体加倍选育毛白杨杂种三倍体[J]. 北京林业大学学报, 2018, 40(11): 12-18. doi: 10.13332/j.1000-1522.20180215
Geng Xining, Ren Yongyu, Han Zhiqiang, Du Kang, Kang Xiangyang. Production of hybrid triploids via inducing chromosome doubling of megaspore with high temperature treatment in Leuce poplar[J]. Journal of Beijing Forestry University, 2018, 40(11): 12-18. doi: 10.13332/j.1000-1522.20180215
Citation: Geng Xining, Ren Yongyu, Han Zhiqiang, Du Kang, Kang Xiangyang. Production of hybrid triploids via inducing chromosome doubling of megaspore with high temperature treatment in Leuce poplar[J]. Journal of Beijing Forestry University, 2018, 40(11): 12-18. doi: 10.13332/j.1000-1522.20180215

高温诱导大孢子染色体加倍选育毛白杨杂种三倍体

doi: 10.13332/j.1000-1522.20180215
基金项目: 

“十三五”国家重点研发计划项目 2016YFD0600403

详细信息
    作者简介:

    耿喜宁,博士生。主要研究方向:林木倍性育种。Email:gengxn@bjfu.edu.cn   地址:100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    康向阳,教授,博士生导师。主要研究方向:林木倍性育种与细胞遗传学。Email:kangxy@bjfu.edu.cn   地址:同上

  • 中图分类号: S792.117

Production of hybrid triploids via inducing chromosome doubling of megaspore with high temperature treatment in Leuce poplar

  • 摘要: 目的鉴于杨树三倍体具有突出的营养生长优势,针对毛白杨良种选育中母本效应难以充分利用问题,选择毛白杨育性好的雌株诱导大孢子染色体加倍选育白杨杂种三倍体,为毛白杨三倍体育种提供技术支撑。方法本研究以毛白杨无性系MC1和MC2为母本,银腺杨YX1为父本。根据白杨大小孢子发生发育存在相关性的原理,以雄株小孢子发育进程为即时判别大孢子发生进程的参照,当小孢子发育至单核早期、单核靠边期和双核期时,分别施加38、40和42 ℃高温持续处理雌花序4 h。结果两个杂交组合的处理组共得到189株毛白杨杂种三倍体,其中MC1×YX1组合中共获得70株杂种三倍体,平均诱导率为56.45%;MC2×YX1组合中共获得119株杂种三倍体,平均诱导率为47.04%,对照组未获得杂种三倍体。当小孢子发育至单核靠边期,对应大孢子母细胞减数分裂至粗线期至中期Ⅰ,此时施加38和40 ℃高温处理4 h是毛白杨大孢子染色体加倍的最佳处理时期。两个杂交组合的杂种三倍体在平均苗高和地径生长方面均优于二倍体,但组合间存在一定差异,表明三倍体育种同样需要重视亲本选配。结论研究进一步证明高温诱导大孢子染色体加倍是一种高效获得毛白杨杂种三倍体的技术途径,相关技术进步对于推进毛白杨良种选育进程具有重要的意义。

     

  • 图  1  毛白杨×银腺杨杂种三倍体倍性鉴定

    三倍体与二倍体植株叶片细胞混合液流式细胞仪检测结果,其中2X表示二倍体,3X表示三倍体。

    Figure  1.  Ploidy identification of P. tomentosa×(P. alba × P. glandulosa) hybrid triploids

    Flow cytometric detection results of mixed foliage cell liquor of diploid and triploid plants, in the picture, 2X represents diploid and 3X represents triploid.

    图  2  毛白杨参照雄株MX1、MX2小孢子发育阶段显微观察

    A.单核小孢子早期;B.单核靠边期;C.双核期。比例尺=5 μm。

    Figure  2.  Microscopical observation on the microspore development stage of reference male MX1 and MX2 of P. tomentosa

    A, early stage of single nucleus; B, late uninucleate stage; C, binucleate stage. Bar=5 μm.

    图  3  毛白杨MC2×YX1一年生苗木苗高地径对比

    Figure  3.  Comparison diagram of height and diameter of 1-year-old P. tomentosa×(P. alba × P. glandulosa) seedlings

    表  1  高温诱导大孢子染色体加倍获得毛白杨MC1×YX1三倍体

    Table  1.   Triploid P. tomentosa MC1× (P. alba×P. glandulosa) YX1 produced by inducing the chromosome doubling of megaspore with high temperature treatment

    小孢子发育状态
    Microspore development status
    处理温度Treatment temperature/℃处理时长Treatment duration/h种子粒数Seed amount成苗株数Seedling amount三倍体株数Triploid amount三倍体得率Triploid yieldrate/%
    小孢子单核早期
    Early stage of single nucleus
    384819778.78
    4038100
    42143181583.33
    单核靠边期
    Late uninucleate stage
    38465922100
    40758864552.33
    420000
    双核期
    Binucleate stage
    38496000
    40281400
    42934125.00
    对照Control8236700
    合计Total2 97219170
    下载: 导出CSV

    表  2  高温诱导大孢子染色体加倍获得MC2×YX1三倍体

    Table  2.   Triploid P. tomentosa MC2×(P. alba × P. glandulosa) YX1 produced by inducing the chromosome doubling of megaspore with high temperature treatment

    小孢子发育状态Microspore development status处理温度Treatment temperature/℃处理时长Treatment duration/h种子粒数Seed amount成苗株数Seedling amount三倍体株数Triploid amount三倍体得率Triploid yieldrate/%
    小孢子单核早期Early stage of single nucleus3845212866.67
    405833100
    42501111100
    单核靠边期Late uninucleate stage384573604473.33
    40571734054.79
    4257141178.57
    双核期Binucleate stage3844566123.28
    402031900
    42102000
    对照Control7441100
    合计Total2 866264119
    下载: 导出CSV

    表  3  毛白杨×银腺杨杂种二三倍体一年生苗木生长状况分析

    Table  3.   Analysis on growth status of 1-year P. tomentosa × (P. alba × P. glandulosa) diploid and triploid seedlings

    项目ItemMC1×YX1MC2×YX1
    苗高Seedling height/cm相对值Relative value/%地径Ground diameter/cm相对值Relative value/%苗高Seedling height/cm相对值Relative value/%地径Ground diameter/cm相对值Relative value/%
    三倍体平均值Triploid average69.17±23.43124.868.86±2.52143.1355.24±16.17115.786.99±1.71126.17
    二倍体平均值Diploid average55.40±17.73100.006.19±1.56100.0047.71±14.34100.005.54±1.60100.00
    三倍体变异系数Triploid coefficient of variation/%33.8728.4429.2724.46
    二倍体变异系数Diploid coefficient of variation/%32.0025.2030.0629.63
    t检验t testt=2.942**t=6.822**t=3.637**t=6.398**
    注:**表示差异极显著(P<0.01)。Note: ** means extremely significant difference (P<0.01).
    下载: 导出CSV
  • [1] 康向阳. 毛白杨细胞遗传与三倍体选育[D]. 北京: 北京林业大学, 1996.

    Kang X Y. Study on cytogenetic and allotriploid breeding of Populus tomentosa[D]. Beijing: Beijing Forestry University, 1996.
    [2] 叶培忠.白杨繁殖育种[J].林业科学, 1955, 1(1): 37-46. http://www.cnki.com.cn/Article/CJFDTOTAL-BJLY201607001.htm

    Ye P Z. Poplar breeding[J]. Scientia Silvae Sinicae, 1995, 1(1): 37-46. http://www.cnki.com.cn/Article/CJFDTOTAL-BJLY201607001.htm
    [3] 朱之悌.全国毛白杨优树资源收集、保存和利用的研究[J].北京林业大学学报, 1992, 14(增刊3): 1-25. http://bjly.chinajournal.net.cn/WKC/WebPublication/paperDigest.aspx?paperID=e121682d-3db3-4dc8-a997-bc9deb141e1c

    Zhu Z T. Collection, conservation and utilization of plus tree resources of Populus tomentosa in China[J]. Journal of Beijing Forestry University, 1992, 14(Suppl. 3): 1-25. http://bjly.chinajournal.net.cn/WKC/WebPublication/paperDigest.aspx?paperID=e121682d-3db3-4dc8-a997-bc9deb141e1c
    [4] 林惠斌, 朱之悌.毛白杨杂交育种战略的研究[J].北京林业大学学报, 1988, 10(3): 97-101. http://www.cnki.com.cn/Article/CJFDTOTAL-BJLY198803014.htm

    Lin H B, Zhu Z T. Studies on breeding strategies of Populus tomentosa[J]. Journal of Beijing Forestry University, 1988, 10(3): 97-101. http://www.cnki.com.cn/Article/CJFDTOTAL-BJLY198803014.htm
    [5] 朱之悌, 林惠斌, 康向阳.毛白杨异源三倍体B301等无性系选育的研究[J].林业科学, 1995, 31(6): 499-505. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199500347545

    Zhu Z T, Lin H B, Kang X Y. Studies on allotriploid breeding of Populus tomentosa B301 clones[J]. Scientia Silvae Sinicae, 1995, 31(6): 499-505. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199500347545
    [6] 张志毅, 李凤兰.白杨染色体加倍技术研究及三倍体育种(Ⅰ):花粉染色体加倍技术[J].北京林业大学学报, 1992, 14(增刊3): 52-58. http://bjly.chinajournal.net.cn/WKC/WebPublication/paperDigest.aspx?paperID=ae4e0519-53d0-46c4-902c-618e5b3bd30a

    Zhang Z Y, Li F L. Studies on chromosome doubling and triploid breeding of white poplar(Ⅰ): the techniques of the pollen chromosome doubling[J]. Journal of Beijing Forestry University, 1992, 14(Suppl.3): 52-58. http://bjly.chinajournal.net.cn/WKC/WebPublication/paperDigest.aspx?paperID=ae4e0519-53d0-46c4-902c-618e5b3bd30a
    [7] 康向阳, 朱之悌, 林惠斌.杨树花粉染色体加倍有效处理时期的研究[J].林业科学, 1999, 35(4): 21-24. doi: 10.3321/j.issn:1001-7488.1999.04.004

    Kang X Y, Zhu Z T, Lin H B. Study on the effective treating period for pollen chromosome doubling of Populus tomentosa×P. bolleana[J]. Scientia Silvae Sinicae, 1999, 35(4): 21-24. doi: 10.3321/j.issn:1001-7488.1999.04.004
    [8] 康向阳, 朱之悌, 张志毅.高温诱导白杨2n花粉有效处理时期的研究[J].北京林业大学学报, 2000, 22(3): 1-4. doi: 10.3321/j.issn:1000-1522.2000.03.001

    Kang X Y, Zhu Z T, Zhang Z Y. Suitable period of high temperature treatment for 2n pollen of Populus tomentosa×P. bolleana[J]. Journal of Beijing Forestry University, 2000, 22(3): 1-4. doi: 10.3321/j.issn:1000-1522.2000.03.001
    [9] 李云, 朱之悌, 田砚亭, 等.极端温度处理白杨雌花芽培育三倍体植株的研究[J].北京林业大学学报, 2000, 22(5): 7-12. doi: 10.3321/j.issn:1000-1522.2000.05.002

    Li Y, Zhu Z T, Tian Y T, et al. Obtaining triploids by high and low temperature treating female flower buds of white poplar[J]. Journal of Beijing Forestry University, 2000, 22(5): 7-12. doi: 10.3321/j.issn:1000-1522.2000.05.002
    [10] 康向阳, 朱之梯, 张志毅.银腺杨与毛新杨正反交三倍体选育[J].北京林业大学学报, 2000, 22(6): 8-11. doi: 10.3321/j.issn:1000-1522.2000.06.003

    Kang X Y, Zhu Z T, Zhang Z Y. Breeding of triploids by the reciprocal crossing of Populus alba × P. glandulosa and P. tomentosa×P. bolleana[J]. Journal of Beijing Forestry University, 2000, 22(6): 8-11. doi: 10.3321/j.issn:1000-1522.2000.06.003
    [11] 康向阳, 张平冬, 高鹏, 等.秋水仙碱诱导白杨三倍体新途径的发现[J].北京林业大学学报, 2004, 26(1): 1-4. http://d.old.wanfangdata.com.cn/Periodical/bjlydxxb200401001

    Kang X Y, Zhang P D, Gao P, et al. Discovery of a new way of poplar triploids induced with colchicine after pollination[J]. Journal of Beijing Forestry University, 2004, 26(1) : 1-4. http://d.old.wanfangdata.com.cn/Periodical/bjlydxxb200401001
    [12] 李艳华, 康向阳.诱导大孢子染色体加倍选育白杨杂种三倍体[J].北京林业大学学报, 2007, 29(5): 22-25. doi: 10.3321/j.issn:1000-1522.2007.05.007

    Li Y H, Kang X Y. Triploid induction in white poplar by chromosome doubling of megaspore[J]. Journal of Beijing Forestry University, 2007, 29(5): 22-25. doi: 10.3321/j.issn:1000-1522.2007.05.007
    [13] 康宁, 白凤莹, 张平冬, 等.高温诱导胚囊染色体加倍获得毛白杨杂种三倍体[J].北京林业大学学报, 2015, 37(2): 79-86. doi: 10.13332/j.cnki.jbfu.2015.02.021

    Kang N, Bai F Y, Zhang P D, et al. Inducing chromosome doubling of embryo sac in Populus tomentosa with high temperature exposure for hybrid triploids[J]. Journal of Beijing Forestry University, 2015, 37(2): 79-86. doi: 10.13332/j.cnki.jbfu.2015.02.021
    [14] 康向阳, 王君.杨树多倍体诱导技术研究[M].北京:科学出版社, 2010.

    Kang X Y, Wang J. Poplar polyploid induction technology research[M]. Beijing: Science Press, 2010.
    [15] 张妍, 崔彬彬, 魏俊杰, 等.毛白杨大、小孢子母细胞减数分裂进程的相关性[J].西北农林科技大学学报(自然科学版), 2015, 43(11): 63-70. http://d.old.wanfangdata.com.cn/Periodical/xbnydxxb201511010

    Zhang Y, Cui B B, Wei J J, et al. Correlation between megesporecytes and microsporecytes meiosis stage of Populus tomentosa Carr.[J]. Journal of Northwest A & F University (Nat. Sci. Ed.), 2015, 43(11): 63-70. http://d.old.wanfangdata.com.cn/Periodical/xbnydxxb201511010
    [16] Bai F Y, Kang N, Zhang P D, et al. Selection of female parents with high fertility and high combining abilities for cross-breeding Populus tomentosa[J/OL]. Journal of Forest Research, 2018[2018-07-05]. https://link.springer.com/content/pdf/10.1007%2Fs11676-018-0684-8.pdf.
    [17] Galbraith D W, Harkins K R, Maddox J M, et al. Rapid flow cytometric analysis of the cell cycle in intact plant tissues[J]. Science, 1983, 220: 1049-1051. doi: 10.1126/science.220.4601.1049
    [18] Wang J, Li D L, Kang X Y. Induction of unreduced megaspores with high temperature during megasporogenesis in Populus[J]. Annals of Forest Science, 2011, 69(1): 59-67. http://d.old.wanfangdata.com.cn/Conference/8447539
    [19] Lu M, Zhang P D, Kang X Y. Induction of 2n female gametes in Populus adenopoda Maxim by high temperature exposure during female gametophyte development[J]. Breed Science, 2013, 63(1): 96-103. doi: 10.1270/jsbbs.63.96
    [20] 康向阳.关于杨树多倍体育种的几点认识[J].北京林业大学学报, 2010, 32(5): 149-153. http://d.old.wanfangdata.com.cn/Periodical/bjlydxxb201005026

    Kang X Y. Some understanding on polyploid breeding of poplars[J]. Journal of Beijing Forestry University, 2010, 32(5): 149-153. http://d.old.wanfangdata.com.cn/Periodical/bjlydxxb201005026
    [21] Ramanna M S, Jacobsen E. Relevance of sexual polyploidization for crop improvement: a review[J]. Euphytica, 2003, 133(1): 3-8. doi: 10.1023/A:1025600824483
    [22] 王君. 青杨派树种多倍体诱导技术研究[D]. 北京: 北京林业大学, 2009. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y1489837

    Wang J. Techniques of polyploid induction in Populus spp. (Section Tacamahaca)[D]. Beijing: Beijing Forestry University, 2009. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y1489837
    [23] Wahid A, Gelani S, Ashraf M, et al. Heat tolerance in plants: an overview[J]. Environmental & Experimental Botany, 2007, 61(3): 199-223. doi: 10.1016-j.envexpbot.2007.05.011/
    [24] Einspahr D W. Production and utilization of triploid hybrid aspen[J]. Iowa State J Res, 1984, 58(4): 401-409.
    [25] Weisgerber H, Rau H M, Gartner E J, et al. 25 years of forest tree breeding in Hesse[J]. Allge Forstze, 1980, 26: 665-712.
    [26] Liao T, Cheng S, Zhu X, et al. Effects of triploid status on growth, photosynthesis, and leaf area in Populus[J]. Trees, 2016, 30(4):1137-1147. doi: 10.1007/s00468-016-1352-2
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  • 收稿日期:  2018-07-02
  • 修回日期:  2018-08-19
  • 刊出日期:  2018-11-01

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