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高产优质毛白杨良种施肥响应特性解析

宋跃朋 肖亮 卜琛皞 卢文杰 刘鹏 张德强

宋跃朋, 肖亮, 卜琛皞, 卢文杰, 刘鹏, 张德强. 高产优质毛白杨良种施肥响应特性解析[J]. 北京林业大学学报, 2019, 41(8): 19-27. doi: 10.13332/j.1000-1522.20180427
引用本文: 宋跃朋, 肖亮, 卜琛皞, 卢文杰, 刘鹏, 张德强. 高产优质毛白杨良种施肥响应特性解析[J]. 北京林业大学学报, 2019, 41(8): 19-27. doi: 10.13332/j.1000-1522.20180427
Song Yuepeng, Xiao Liang, Bu Chenhao, Lu Wenjie, Liu Peng, Zhang Deqiang. Analysis of the fertilization response characteristics of improved variety for high yield and high quality Populus tomentosa[J]. Journal of Beijing Forestry University, 2019, 41(8): 19-27. doi: 10.13332/j.1000-1522.20180427
Citation: Song Yuepeng, Xiao Liang, Bu Chenhao, Lu Wenjie, Liu Peng, Zhang Deqiang. Analysis of the fertilization response characteristics of improved variety for high yield and high quality Populus tomentosa[J]. Journal of Beijing Forestry University, 2019, 41(8): 19-27. doi: 10.13332/j.1000-1522.20180427

高产优质毛白杨良种施肥响应特性解析

doi: 10.13332/j.1000-1522.20180427
基金项目: 国家林业和草原局科技成果推广计划项目([2016]28),国家自然科学基金项目(31400553、31770707)
详细信息
    作者简介:

    宋跃朋,博士,副教授。主要研究方向:林木分子设计育种。Email:YuepengSong@bjfu.edu.cn 地址:100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    张德强,教授,博士生导师。主要研究方向:林木分子设计育种。Email:DeqiangZhang@bjfu.edu.cn 地址:同上

  • 中图分类号: S722.8;S792.117

Analysis of the fertilization response characteristics of improved variety for high yield and high quality Populus tomentosa

  • 摘要: 目的探寻林木最佳栽培方法是切实提高森林生产力的重要举措。由于毛白杨是杂种起源,其种内无性系间施肥响应特性差异显著。为保证人工林建设的速生丰产特性,同时减轻环境保护压力,应该大量推广毛白杨良种配制最佳施肥配比。方法以3个毛白杨良种为研究对象,利用不同施肥处理探明影响其重要生长性状的最佳施肥配比,并对高产优质毛白杨良种施肥响应特性相关的候选基因表达模式进行系统解析。结果‘毅杨1号’最佳施肥配比为N(3 g) + P2O5(2. 25 g) + K2O(1 g);‘毅杨2号’最佳施肥配比为N(3 g) + P2O5(2. 25 g) + K2O(0.5 g);‘毅杨3号’最佳施肥配比为N(7 g) + P2O5(2. 25 g) + K2O(0.5 g)。其中,氮元素是影响毛白杨毅杨良种的苗高、地径以及生物量最为显著的营养元素。为进一步解析其遗传调控基础,对与氮素运输、同化、代谢相关的16个重要候选基因在不同氮肥施肥水平下在叶片以及根系中的表达模式进行了解析。候选基因表达模式分析结果表明,毛白杨良种氮素相关候选基因在叶片以及根中聚类成不同的模块,并且无性系之间表达模式差异显著。结论本研究阐明了毅杨毛白杨良种的施肥响应特性,系统分析其转录调控特异性将有利于解析其氮肥响应特性形成的分子机制,并对进一步针对氮素吸收利用效率开展遗传改良的分子设计育种具有重要意义。

     

  • 图  1  高产优质毛白杨毅杨良种不同施肥水平的苗高生长特性

    Figure  1.  Seedling height growth characteristics of improved variety for high yield and high quality P. tomentosa

    图  2  高产优质毛白杨毅杨良种苗高生长影响显著元素水平的多重比较

    Figure  2.  Multiple comparisons of significant elemental levels in the height growth of improved variety for high yield and high quality P. tomentosa

    图  3  高产优质毛白杨良种地径生长影响显著元素水平的多重比较

    Figure  3.  Multiple comparisons of significant elemental levels in the diameter growth of improved variety for high yield and high quality P. tomentosa

    图  4  高产优质毛白杨良种生物量影响显著元素水平的多重比较

    Figure  4.  Multiple comparisons of significant elemental levels in the biomass of improved variety for high yield and high quality P. tomentosa

    图  5  氮运输、同化、代谢相关候选基因表达模式

    Figure  5.  Nitrogen transport, assimilation, metabolism related candidate gene expression patterns

    表  1  氮、磷、钾施肥水平及全年施肥量

    Table  1.   Fertilization levels and the fertilization amounts in the whole year g

    施肥水平 Total fertilization level 施肥总量 Fertilization amount
    N P2O5 K2O
    1 3 0 0
    2 5 2.25 0.5
    3 7 4.5 1
    下载: 导出CSV

    表  2  施肥处理

    Table  2.   The fertilizing treatment g

    处理 Treatment N P2O5 K2O
    1 3 0 0
    2 3 2.25 0.5
    3 3 4.5 1
    4 5 4.5 0.5
    5 5 0 1
    6 5 2.25 0
    7 7 2.25 1
    8 7 4.5 0
    9 7 0 0.5
    下载: 导出CSV

    表  3  氮运输、同化、代谢候选基因荧光定量引物序列列表

    Table  3.   Real time-PCR primer sequence list of nitrogen transport, assimilation, metabolic candidate gene

    基因模型  
    Gene model  
    基因名称
    Gene name
    引物序列
    Primer sequences
    Potri.009G045200 AMT1.6 F:5′-TTCAGGTGAGGCGGGAGT-3′ R: 5′-CCAAAGCGCCAATGATACAG-3′
    Potri.006G102800 AMT2.1 F:5′-GTTATAGGTTGGAACGTGGTGT-3′ R: 5′-TCAATATTACATGCGTCAAGATTC-3′
    Potri.003G111500 NRT1.1 F:5′-CTAAACCAAGGGAGGCTCCATGAT-3′ R: 5′-CCCAACACAAAAGTAGGCGAAAAG-3′
    Potri.012G070700 NRT1.2 F:5′-TCTTTGGTAGCAACTTGAACAA-3′ R: 5′-TCTCTCTCTCTCTCGTCTCCCT-3′
    Potri.009G008500 NRT2.4B F:5′-AATAGAGGAAGGGAATGGCTG-3′ R: 5′-TGAGGTTGTCCCGAATGATAG-3′
    Potri.009G008600 NRT2.4C F:5′-CAGTCCCGACAGATACAAC-3′ R: 5′-CTTCCCACTACAACGATTTC-3′
    Potri.015G085000 NRT3.1B F:5′-TCATAGCCTCTTCTTCTACCTTTCC-3′ R: 5′-CCACCTTTCAATACTTGTCCG-3′
    Potri.015G085100 NRT3.1C F:5′-AGAGGTCTCAGTGAAGCGAACAAG-3′ R: 5′-CGCAAATACAAACGCAATTATCAT-3′
    Potri.005G172400 NR F:5′-ATCATCGGATCGGAGAGTTGG-3′ R: 5′-GACGGT-GCTAGTTGGCGTATAG-3′
    Potri.004G140800 NiR F:5′-ACAAGTTGCCGATATTGGGTTCAT-3′ R: 5′-CCTCTATCACCCGTCGTAGTCCTG-3′
    Potri.017G131100 GS1.3 F: 5′-GGCGCAGACCAAGCTTCTC-3′ R: 5′-GCGAAGTGACAGATTTAGGATTGC-3′
    Potri.010G029100 GS2 F: 5′-ATCAGGTGGGTCCCAGTGTG-3′ R: 5′-GCGAAGTGACAGATTTAGGATTGC-3′
    Potri.016G036900 Fd-GOGAT F: 5′-AACCCAAAGGCATCAGACTCAG-3′ R: 5′-AGTAAAGCAGGTCCATCCCAAG-3′
    Potri.012G011700 NADH-GOGAT F: 5′-GGTGTTGTGGATATTCCTCCTG-3′ R: 5′-TCAGATGCGGCGACAACCC-3′
    Potri.013G058300 GDH F: 5′-ATTTATGCTAACTCAGGAGGCGTT-3′ R: 5′-GAGGAGGAAACTAGGGCAATACAT-3′
    Potri.018G006000 VHA1.1 F: 5′-CCGCTCAGACCAACACTATCTT-3′ R: 5′-TCCTCTGGGCTTGAATGGTAG-3′
    Potri.018G090300 VHA2.2 F: 5′-GGCTCCAACCACCTGAGACAG -3′ R: 5′-AGCACAGCCCTTTCTCTTCCA-3′
    内参基因 Normalized gene Actin 2/7 F: 5′-CCCATTGAGCACGGTATTGT-3′ R: 5′-TACGACCACTGGCATACAGG-3′
    下载: 导出CSV

    表  4  不同施肥处理对高产优质毛白杨良种无性系苗高的影响

    Table  4.   Effects of different fertilization treatments on clone seedling height of improved variety for high yield and high quality P. tomentosa cm

    处理
    Treatment
    ‘毅杨1号’
    ‘Yiyang 1’
    ‘毅杨2号’
    ‘Yiyang 2’
    ‘毅杨3号’
    ‘Yiyang 3’
    1 176.85 ± 11.25B 192.35 ± 13.15AB 180.1 ± 5.12AB
    2 215.22 ± 8.13A 219.13 ± 10.19A 189.22 ± 5.09A
    3 211.55 ± 3.35A 209.17 ± 8.51AB 167.22 ± 4.71C
    4 215.12 ± 10.14A 165.14 ± 7.197C 175.18 ± 6.2BC
    5 185.71 ± 7.31B 173.87 ± 10.15C 150.91 ± 5.13CD
    6 212.25 ± 9.54A 229.45 ± 2.51A 164.13 ± 8.23CD
    7 174.55 ± 3.41B 166.25 ± 7.22C 194.12 ± 6.25A
    8 175.16 ± 11.25B 197.05 ± 7.15AB 184.21 ± 5.61AB
    9 129.65 ± 7.15C 183.71 ± 4.23C 163.75 ± 4.13C
    CK 163.2 ± 11.04B 131.82 ± 5.81D 95.3 ± 7.91E
    注:在P < 0. 01水平上差异显著。下同。Notes: significant difference at P < 0. 01 level. Same as below.
    下载: 导出CSV

    表  5  不同施肥处理对高产优质毛白杨毅杨良种无性系地径的影响

    Table  5.   Effects of different fertilization treatments on the clone diameter of improved variety for high yield and high quality P. tomentosa cm

    处理
    Treatment
    ‘毅杨1号’
    ‘Yiyang 1’
    ‘毅杨2号’
    ‘Yiyang 2’
    ‘毅杨3号’
    ‘Yiyang 3’
    1 1.14 ± 0.12C 1.58 ± 0.13A 1.39 ± 0.09B
    2 1.63 ± 0.06A 1.66 ± 0.12A 1.72 ± 0.08A
    3 1.62 ± 0.04A 1.62 ± 0.05A 1.55 ± 0.13A
    4 1.60 ± 0.05A 1.33 ± 0.12AB 1.69 ± 0.11A
    5 1.69 ± 0.07A 1.38 ± 0.14AB 1.24 ± 0.03C
    6 1.71 ± 0.12A 1.60 ± 0.13A 1.63 ± 0.07A
    7 1.47 ± 0.14AB 1.30 ± 0.12B 1.71 ± 0.07A
    8 1.52 ± 0.06AB 1.60 ± 0.04A 1.84 ± 0.13A
    9 1.12 ± 0.05C 1.40 ± 0.06B 1.67 ± 0.07A
    CK 1.13 ± 0.12C 0.99 ± 0.06C 0.97 ± 0.06D
    下载: 导出CSV

    表  6  不同施肥处理对高产优质毛白杨毅杨良种无性系生物量的影响

    Table  6.   Effects of different fertilization treatments on the clone biomass of improved variety for high yield and high quality P. tomentosa cm

    处理
    Treatment
    ‘毅杨1号’
    ‘Yiyang 1’
    ‘毅杨2号’
    ‘Yiyang 2’
    ‘毅杨3号’
    ‘Yiyang 3’
    1 98.16 ± 8.21BC 121.05 ± 11.58A 111.97 ± 3.21C
    2 112.36 ± 10.14AB 124.51 ± 12.06A 143.82 ± 6.92A
    3 134.24 ± 8.83A 113.61 ± 10.28A 95.73 ± 5.21D
    4 125.23 ± 11.65AB 123.24 ± 11.21A 138.8 ± 7.58A
    5 101.35 ± 12.81BC 95.31 ± 9.65BC 72.35 ± 4.95E
    6 134.18 ± 6.75A 125.56 ± 4.54A 78.46 ± 7.21E
    7 103.81 ± 12.24BC 61.49 ± 8.15D 143.25 ± 4.33A
    8 92.21 ± 8.11C 106.78 ± 4.16B 130.14 ± 9.52A
    9 59.18 ± 2.43D 82.65 ± 6.71BC 113.86 ± 8.12B
    CK 52.97 ± 4.87D 51.38 ± 5.16D 29.12 ± 3.41F
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-12-20
  • 修回日期:  2019-05-08
  • 网络出版日期:  2019-07-17
  • 刊出日期:  2019-08-01

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