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高产优质毛白杨良种组培繁育体系构建

宋跃朋 陈盼飞 卜琛皞 轩安然 张德强

宋跃朋, 陈盼飞, 卜琛皞, 轩安然, 张德强. 高产优质毛白杨良种组培繁育体系构建[J]. 北京林业大学学报, 2019, 41(7): 121-127. doi: 10.13332/j.1000-1522.20180428
引用本文: 宋跃朋, 陈盼飞, 卜琛皞, 轩安然, 张德强. 高产优质毛白杨良种组培繁育体系构建[J]. 北京林业大学学报, 2019, 41(7): 121-127. doi: 10.13332/j.1000-1522.20180428
Song Yuepeng, Chen Panfei, Bu Chenhao, Xuan Anran, Zhang Deqiang. Construction of tissue culture breeding system of high yield and quality species in Populus tomentosa[J]. Journal of Beijing Forestry University, 2019, 41(7): 121-127. doi: 10.13332/j.1000-1522.20180428
Citation: Song Yuepeng, Chen Panfei, Bu Chenhao, Xuan Anran, Zhang Deqiang. Construction of tissue culture breeding system of high yield and quality species in Populus tomentosa[J]. Journal of Beijing Forestry University, 2019, 41(7): 121-127. doi: 10.13332/j.1000-1522.20180428

高产优质毛白杨良种组培繁育体系构建

doi: 10.13332/j.1000-1522.20180428
基金项目: 国家林业和草原局科技成果推广计划项目([2016]28),国家自然科学基金项目(31400553、31770707)
详细信息
    作者简介:

    宋跃朋,副教授。主要研究方向:林木分子设计育种。Email:YuepengSong@bjfu.edu.cn 地址:100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    张德强,教授,博士生导师。主要研究方向:林木分子设计育种。Email:DeqiangZhang@bjfu.edu.cn 地址:同上

  • 中图分类号: S722.8;S792.117

Construction of tissue culture breeding system of high yield and quality species in Populus tomentosa

  • 摘要: 目的毛白杨是我国北方人工林营造、景观绿化及木材加工等产业应用的主要树种。近年来,由于林业产业升级,原有的毛白杨多圃配套繁育体系不能满足高质量毛白杨良种的快速繁育需求,因此亟需构建高效的毛白杨良种繁育技术体系以保障毛白杨良种在木材生产以及城市绿化中的广泛应用。方法以高产优质毛白杨良种‘毅杨1号’‘毅杨2号’和‘毅杨3号’为研究对象,以其茎段和叶片为试验材料,利用组织培养技术构建毛白杨良种组培繁育体系。本研究系统地分析了‘毅杨 1 号’ ‘毅杨2号’和‘毅杨3号’外植体的消毒方法及植物激素对其不定芽、生根及叶片增殖诱导过程中的作用。结果(1)75%乙醇处理30 s联合2%次氯酸钠处理5 min是3个毛白杨良种外植体的最佳消毒方法。(2)不定芽诱导中0.3 mg/L 6-BA和0.1 mg/L的NAA适用于‘毅杨 1 号’和‘毅杨2号’ ‘毅杨3号’则在0.5 mg/L 6-BA和0.1 mg/L NAA的条件下增殖系数达到最大。(3)生根诱导中‘毅杨 1 号’和‘毅杨3号’的IBA最适质量浓度为0.5 mg/L,‘毅杨2号’则为0.3 mg/L。(4)叶片增殖结果显示,‘毅杨 1 号’和‘毅杨3号’的植物激素最适质量浓度为1.0 mg/L 6-BA和0.1 mg/L NAA,‘毅杨2号’为0.7 mg/L 6-BA和0.1 mg/L NAA。结论本研究构建了‘毅杨 1 号’ ‘毅杨2号’ ‘毅杨3号’的快速繁育体系,可用于毛白杨良种的快速繁殖以及遗传转化研究,并将为毛白杨良种推广应用以及进一步利用基因工程技术在分子水平对现有良种开展精准改良奠定了基础。

     

  • 图  1  高产优质毛白杨良种茎段最适增殖培养基上的生长状况

    Figure  1.  Optimum growth conditions of stem segments of high yield and quality elite of P. tomentosa and their performance

    图  2  高产优质毛白杨树良种茎段在最适生根培养基上的生根状况

    Figure  2.  Optimum growth conditions of rooting medium for high yield and quality elite of P. tomentosa and their performance

    图  3  高产优质毛白杨树良种叶片最适分化培养基上的生长状况

    Figure  3.  Optimum condition of leaf differentiation medium for high yield and quality elite of P. tomentosa and their performance

    表  1  外植体消毒处理

    Table  1.   Sterilization treatment of explants

    编号
    No.
    75%乙醇处理时间
    Treating time of 75% ethanol/s
    2%次氯酸钠处理时间
    Treating time of 2% sodium hypochlorite/min
    1 30 3
    2 30 5
    3 30 7
    4 60 3
    5 60 5
    6 60 7
    下载: 导出CSV

    表  2  不定芽诱导培养基中激素水平

    Table  2.   Hormone levels in adventitious bud induction medium mg·L− 1

    编号 No.6-BANAA
    1 0.1 0.1
    2 0.3 0.1
    3 0.5 0.1
    4 0.7 0.1
    下载: 导出CSV

    表  3  消毒处理时间对毛白杨良种外植体的影响

    Table  3.   Effects of sterilization time treatment on explants of poplar

    编号
    No.
    75%乙醇处理时间
    Treating time of 75%
    ethyl alcohol/s
    2%次氯酸钠处理时间
    Treating time of 2% sodium
    hypochlorite/min
    污染死亡率 Pollution mortality rate/%
    ‘毅杨1号’ ‘Yiyang 1’‘毅杨2号’ ‘Yiyang 2’‘毅杨3号’ ‘Yiyang 3’
    130351.7ab 43.3bc 34.5bc
    230520.7c 23.4c 18.8d
    330746.7bc 49.1abc32.6bc
    460358.3a 53.2ab 38.8b
    560550.4abc48.8abc22.6bc
    660753.3ab 60.1a 47.5a
    注:同一列中不同字母表示差异显著(P < 0. 05)。下同。Notes: different letters indicate significant difference at P < 0. 05 level. The same below.
    下载: 导出CSV

    表  4  激素质量浓度对毛白杨良种不定芽诱导的影响

    Table  4.   Effects of mass concentrations of hormones on adventitious bud induction

    编号
    No.
    6-BA/(mg·L− 1)NAA/(mg·L− 1)增殖系数 Multiplication coefficient/%
    ‘毅杨1号’ ‘Yiyang 1’‘毅杨2号’ ‘Yiyang 2’‘毅杨3号’ ‘Yiyang 3’
    10.10.162.0bc55.6bc31.5c
    20.30.183.3a 77.4a 40.2bc
    30.50.179.1b 60.2b 80.6a
    40.70.154.1c 44.8c 52.9b
    下载: 导出CSV

    表  5  激素质量浓度对毛白杨良种生根的影响

    Table  5.   Effects of hormone mass concentration on rooting condition of high yield and quality elite of P. tomentosa

    编号
    No.
    IBA/(mg·L− 1)生根率 Rooting percentage/%
    ‘毅杨1号’ ‘Yiyang 1’‘毅杨2号’ ‘Yiyang 2’‘毅杨3号’ ‘Yiyang 3’
    1 0.1 21.67 ± 3.33b 23.33 ± 4.41d 31.67 ± 3.56c
    2 0.3 36.67 ± 3.31b 98.33 ± 5.01a 91.67 ± 4.24a
    3 0.5 85.12 ± 5.77a 86.67 ± 4.41b 97.02 ± 1.51a
    4 0.7 68.33 ± 6.01a 55.00 ± 5.77c 66.67 ± 4.43b
    下载: 导出CSV

    表  6  激素质量浓度对毛白杨良种叶片增殖的影响

    Table  6.   Effects of hormone mass concentration on leaf proliferation of P. tomentosa

    编号
    No.
    6-BA/(mg·L− 1)NAA/(mg·L− 1)出芽率 Budding rate/%
    ‘毅杨1号’ ‘Yiyang 1’‘毅杨2号’ ‘Yiyang 2’‘毅杨3号’ ‘Yiyang 3’
    1 0.3 0.1 20.10 ± 3.11d 18.65 ± 2.22d 26.15 ± 3.89d
    2 0.5 0.1 55.45 ± 3.44c 60.23 ± 4.52bc 30.56 ± 6.41c
    3 0.7 0.1 72.3 ± 4.21b 92.10 ± 4.65a 70.39 ± 5.21b
    4 1.0 0.1 95.8 ± 1.02a 65.45 ± 3.78b 90.77 ± 6.12a
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-12-20
  • 修回日期:  2019-05-08
  • 网络出版日期:  2019-07-01
  • 刊出日期:  2019-07-01

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